(Linear) (Six-base) MAPSORT of: KIEE6421.seq Check: 4532 from: 1 to: 1674 KIEE6421.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 153 1674 Size: 153 1521 AccI GT'mk_AC Cuts at: 0 44 1195 1674 Size: 44 1151 479 Fragments arranged by size: 1151 479 44 Acc65I G'GTAC_C Cuts at: 0 1599 1674 Size: 1599 75 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 1674 Size: 351 1323 AfeI AGC'GCT Cuts at: 0 697 1292 1674 Size: 697 595 382 Fragments arranged by size: 697 595 382 AflIII A'CryG_T Cuts at: 0 1244 1246 1674 Size: 1244 2 428 Fragments arranged by size: 1244 428 2 AgeI A'CCGG_T Cuts at: 0 1596 1624 1674 Size: 1596 28 50 Fragments arranged by size: 1596 50 28 AhdI GACnn_n'nnGTC Cuts at: 0 426 642 912 1674 Size: 426 216 270 762 Fragments arranged by size: 762 426 270 216 AleI CACnn'nnGTG Cuts at: 0 850 1231 1674 Size: 850 381 443 Fragments arranged by size: 850 443 381 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1120 1674 Size: 1120 554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 196 228 1674 Size: 196 32 1446 Fragments arranged by size: 1446 196 32 AlwNI CAG_nnn'CTG Cuts at: 0 609 1475 1674 Size: 609 866 199 Fragments arranged by size: 866 609 199 ApaI G_GGCC'C Cuts at: 0 1538 1674 Size: 1538 136 ApaLI G'TGCA_C Cuts at: 0 121 1674 Size: 121 1553 ApoI r'AATT_y Cuts at: 0 544 1674 Size: 544 1130 AvaI C'yCGr_G Cuts at: 0 52 175 1342 1674 Size: 52 123 1167 332 Fragments arranged by size: 1167 332 123 52 BanI G'GyrC_C Cuts at: 0 165 316 390 852 1032 1395 1599 1674 Size: 165 151 74 462 180 363 204 75 Fragments arranged by size: 462 363 204 180 165 151 75 74 BanII G_rGCy'C Cuts at: 0 34 761 864 872 1487 1538 1658 1674 Size: 34 727 103 8 615 51 120 16 Fragments arranged by size: 727 615 120 103 51 34 16 8 BbeI G_GCGC'C Cuts at: 0 394 1036 1399 1674 Size: 394 642 363 275 Fragments arranged by size: 642 394 363 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1437 1471 1674 Size: 1437 34 203 Fragments arranged by size: 1437 203 34 BglI GCCn_nnn'nGGC Cuts at: 0 64 1674 Size: 64 1610 BlpI GC'TnA_GC Cuts at: 0 1378 1674 Size: 1378 296 Bme1580I G_kGCm'C Cuts at: 0 125 170 319 857 1538 1674 Size: 125 45 149 538 681 136 Fragments arranged by size: 681 538 149 136 125 45 BmgBI CAC'GTC Cuts at: 0 39 131 1674 Size: 39 92 1543 Fragments arranged by size: 1543 92 39 BmrI ACTGGGnnnn_n' Cuts at: 0 9 483 1096 1674 Size: 9 474 613 578 Fragments arranged by size: 613 578 474 9 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1215 1674 Size: 1215 459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 787 829 1674 Size: 631 156 42 845 Fragments arranged by size: 845 631 156 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 886 955 1528 1674 Size: 100 786 69 573 146 Fragments arranged by size: 786 573 146 100 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 839 1674 Size: 839 835 BsaAI yAC'GTr Cuts at: 0 1164 1247 1674 Size: 1164 83 427 Fragments arranged by size: 1164 427 83 BsaHI Gr'CG_yC Cuts at: 0 338 391 1033 1396 1674 Size: 338 53 642 363 278 Fragments arranged by size: 642 363 338 278 53 BsaWI w'CCGG_w Cuts at: 0 80 149 641 1596 1624 1674 Size: 80 69 492 955 28 50 Fragments arranged by size: 955 492 80 69 50 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 196 226 478 508 1674 Size: 196 30 252 30 1166 Fragments arranged by size: 1166 252 196 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1666 1674 Size: 1666 8 BseYI C'CCAG_C Cuts at: 0 194 416 1212 1537 1674 Size: 194 222 796 325 137 Fragments arranged by size: 796 325 222 194 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1222 1311 1605 1674 Size: 1111 111 89 294 69 Fragments arranged by size: 1111 294 111 89 69 BsiEI CG_ry'CG Cuts at: 0 276 1674 Size: 276 1398 BsiHKAI G_wGCw'C Cuts at: 0 125 761 864 872 1109 1674 Size: 125 636 103 8 237 565 Fragments arranged by size: 636 565 237 125 103 8 BsiWI C'GTAC_G Cuts at: 0 1160 1674 Size: 1160 514 Bsp1286I G_dGCh'C Cuts at: 0 34 125 170 319 761 857 864 872 Size: 34 91 45 149 442 96 7 8 Cuts at: 872 1109 1487 1538 1658 1674 Size: 237 378 51 120 16 Fragments arranged by size: 442 378 237 149 120 96 91 51 45 34 16 8 7 BspEI T'CCGG_A Cuts at: 0 149 1674 Size: 149 1525 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 153 749 1674 Size: 153 596 925 Fragments arranged by size: 925 596 153 BsrBI CCG'CTC Cuts at: 0 1049 1674 Size: 1049 625 BsrDI GCAATG_nn' Cuts at: 0 1036 1674 Size: 1036 638 BsrFI r'CCGG_y Cuts at: 0 1596 1624 1674 Size: 1596 28 50 Fragments arranged by size: 1596 50 28 BssHII G'CGCG_C Cuts at: 0 456 566 568 570 1179 1181 1674 Size: 456 110 2 2 609 2 493 Fragments arranged by size: 609 493 456 110 2 2 2 BssSI C'ACGA_G Cuts at: 0 403 1674 Size: 403 1271 BstXI CCAn_nnnn'nTGG Cuts at: 0 807 1230 1281 1674 Size: 807 423 51 393 Fragments arranged by size: 807 423 393 51 BstYI r'GATC_y Cuts at: 0 617 1674 Size: 617 1057 BstZ17I GTA'TAC Cuts at: 0 1196 1674 Size: 1196 478 Bsu36I CC'TnA_GG Cuts at: 0 1468 1674 Size: 1468 206 BtgI C'CryG_G Cuts at: 0 227 383 794 1559 1674 Size: 227 156 411 765 115 Fragments arranged by size: 765 411 227 156 115 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 463 534 1674 Size: 463 71 1140 Fragments arranged by size: 1140 463 71 BtsI GCAGTG_nn' Cuts at: 0 1134 1674 Size: 1134 540 DraIII CAC_nnn'GTG Cuts at: 0 1140 1365 1674 Size: 1140 225 309 Fragments arranged by size: 1140 309 225 EaeI y'GGCC_r Cuts at: 0 236 273 440 496 554 797 1265 1674 Size: 236 37 167 56 58 243 468 409 Fragments arranged by size: 468 409 243 236 167 58 56 37 EagI C'GGCC_G Cuts at: 0 273 1674 Size: 273 1401 EarI CTCTTCn'nnn_ Cuts at: 0 793 1118 1393 1674 Size: 793 325 275 281 Fragments arranged by size: 793 325 281 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 914 1232 1429 1674 Size: 914 318 197 245 Fragments arranged by size: 914 318 245 197 EcoICRI GAG'CTC Cuts at: 0 759 862 870 1674 Size: 759 103 8 804 Fragments arranged by size: 804 759 103 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 631 787 829 1674 Size: 351 280 156 42 845 Fragments arranged by size: 845 351 280 156 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 442 1390 1674 Size: 442 948 284 Fragments arranged by size: 948 442 284 EcoO109I rG'GnC_Cy Cuts at: 0 510 1472 1534 1674 Size: 510 962 62 140 Fragments arranged by size: 962 510 140 62 EcoRI G'AATT_C Cuts at: 0 544 1674 Size: 544 1130 FspI TGC'GCA Cuts at: 0 602 748 1674 Size: 602 146 926 Fragments arranged by size: 926 602 146 HaeII r_GCGC'y Cuts at: 0 394 699 1036 1294 1399 1530 1674 Size: 394 305 337 258 105 131 144 Fragments arranged by size: 394 337 305 258 144 131 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 628 660 898 930 1012 1044 Size: 412 32 184 32 238 32 82 32 Cuts at: 1044 1674 Size: 630 Fragments arranged by size: 630 412 238 184 82 32 32 32 32 KasI G'GCGC_C Cuts at: 0 390 1032 1395 1674 Size: 390 642 363 279 Fragments arranged by size: 642 390 363 279 KpnI G_GTAC'C Cuts at: 0 1603 1674 Size: 1603 71 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1533 1674 Size: 1533 141 MscI TGG'CCA Cuts at: 0 238 799 1674 Size: 238 561 875 Fragments arranged by size: 875 561 238 MslI CAynn'nnrTG Cuts at: 0 850 1231 1333 1674 Size: 850 381 102 341 Fragments arranged by size: 850 381 341 102 MspA1I CmG'CkG Cuts at: 0 229 272 609 1451 1606 1674 Size: 229 43 337 842 155 68 Fragments arranged by size: 842 337 229 155 68 43 NarI GG'CG_CC Cuts at: 0 391 1033 1396 1674 Size: 391 642 363 278 Fragments arranged by size: 642 391 363 278 NcoI C'CATG_G Cuts at: 0 383 1674 Size: 383 1291 NotI GC'GGCC_GC Cuts at: 0 273 1674 Size: 273 1401 NspI r_CATG'y Cuts at: 0 1506 1674 Size: 1506 168 PasI CC'CwG_GG Cuts at: 0 1531 1674 Size: 1531 143 PflMI CCAn_nnn'nTGG Cuts at: 0 1140 1674 Size: 1140 534 PfoI T'CCnGG_A Cuts at: 0 806 1508 1674 Size: 806 702 166 Fragments arranged by size: 806 702 166 PmlI CAC'GTG Cuts at: 0 1247 1674 Size: 1247 427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 196 228 1674 Size: 196 32 1446 Fragments arranged by size: 1446 196 32 PshAI GACnn'nnGTC Cuts at: 0 91 928 1674 Size: 91 837 746 Fragments arranged by size: 837 746 91 PspOMI G'GGCC_C Cuts at: 0 1534 1674 Size: 1534 140 PstI C_TGCA'G Cuts at: 0 485 1674 Size: 485 1189 PvuII CAG'CTG Cuts at: 0 609 1451 1674 Size: 609 842 223 Fragments arranged by size: 842 609 223 RsrII CG'GwC_CG Cuts at: 0 152 1674 Size: 152 1522 SacI G_AGCT'C Cuts at: 0 761 864 872 1674 Size: 761 103 8 802 Fragments arranged by size: 802 761 103 8 SacII CC_GC'GG Cuts at: 0 230 1674 Size: 230 1444 SapI GCTCTTCn'nnn_ Cuts at: 0 793 1118 1674 Size: 793 325 556 Fragments arranged by size: 793 556 325 SfcI C'TryA_G Cuts at: 0 481 1674 Size: 481 1193 SfoI GGC'GCC Cuts at: 0 392 1034 1397 1674 Size: 392 642 363 277 Fragments arranged by size: 642 392 363 277 SgrAI Cr'CCGG_yG Cuts at: 0 1624 1674 Size: 1624 50 SmlI C'TyrA_G Cuts at: 0 115 865 970 1543 1674 Size: 115 750 105 573 131 Fragments arranged by size: 750 573 131 115 105 SpeI A'CTAG_T Cuts at: 0 1401 1674 Size: 1401 273 StuI AGG'CCT Cuts at: 0 719 1674 Size: 719 955 StyI C'CwwG_G Cuts at: 0 383 1674 Size: 383 1291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 751 1156 1287 1315 1674 Size: 751 405 131 28 359 Fragments arranged by size: 751 405 359 131 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 115 147 478 510 1674 Size: 115 32 331 32 1164 Fragments arranged by size: 1164 331 115 32 32 Tth111I GACn'n_nGTC Cuts at: 0 40 664 978 1674 Size: 40 624 314 696 Fragments arranged by size: 696 624 314 40 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1071 1674 Size: 1071 603 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NotI NspI PasI PflMI PfoI PmlI PpiI PshAI PspOMI PstI PvuII RsrII SacI SacII SapI SfcI SfoI SgrAI SmlI SpeI StuI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AatII AclI AflII AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI Bpu10I BsaBI BsmI BsmBI BspHI BsrGI BstAPI BstBI BstEII ClaI CspCI DraI DrdI EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PpuMI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SphI SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI ZraI