################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccI AccIII AclI AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI AviII AvrII BcgI BclI BglI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI TfiI XbaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1481 GGTACC G^GTACC AccII 1855 CGCG CG^CG AflII 379 CTTAAG C^TTAAG Alw44I 465 GTGCAC G^TGCAC ApaLI 465 GTGCAC G^TGCAC Asp718I 1481 GGTACC G^GTACC AspI 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1469 TGGCCA TGG^CCA BamHI 207 GGATCC G^GATCC BbsI 1630 GTCTTC GAAGAC(2/6) BbvCI 442 CCTCAGC CCTCAGC(-5/-2) BciVI 1775 GTATCC GTATCC(6/5) BfrI 379 CTTAAG C^TTAAG BpuAI 1630 GTCTTC GAAGAC(2/6) BsaWI 140 ACCGGA [AT]^CCGG[AT] BsiEI 582 CGGCCG CG[GA][CT]^CG BsmI 401 GCATTC GAATGC(1/-1) BspLU11I 1202 ACATGT A^CATGT BsrBI 99 GAGCGG CCGCTC(-3/-3) BssSI 1339 CTCGTG CACGAG(-5/-1) BstEII 795 GGTGACC G^GT[ACGT]ACC BstPI 795 GGTGACC G^GT[ACGT]ACC BstUI 1855 CGCG CG^CG DraIII 984 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 582 CGGCCG C^GGCCG EclXI 582 CGGCCG C^GGCCG Eco52I 582 CGGCCG C^GGCCG EcoO65I 795 GGTGACC G^GT[ACGT]ACC HgaI 2148 GACGC GACGC(5/10) KpnI 1481 GGTACC GGTAC^C KspI 1854 CCGCGG CCGC^GG MluNI 1469 TGGCCA TGG^CCA MscI 1469 TGGCCA TGG^CCA MvnI 1855 CGCG CG^CG NotI 581 GCGGCCGC GC^GGCCGC PaeR7I 2200 CTCGAG C^TCGAG PflFI 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 1795 GAGCTC GAGCT^C SacII 1854 CCGCGG CCGC^GG Tth111I 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1994 CCATCTCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2200 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AcsI 145 AAATTC [GA]^AATT[CT] 1804 AAATTC AcyI 1367 GGCGCC G[GA]^CG[CT]C 1682 GGCGCC Aor51HI 493 AGCGCT AGC^GCT 2099 AGCGCT ApaI 830 GGGCCC GGGCC^C 1760 GGGCCC ApoI 145 AAATTC [GA]^AATT[CT] 1804 AAATTC AspHI 465 GTGCAC G[AT]GC[AT]^C 1795 GAGCTC BbeI 1367 GGCGCC GGCGC^C 1682 GGCGCC BbrPI 1372 CACGTG CAC^GTG 1541 CACGTG BfaI 754 CTAG C^TAG 1321 CTAG BsaHI 1367 GGCGCC G[GA]^CG[CT]C 1682 GGCGCC BsaI 1939 GAGACC GGTCTC(1/5) 246 GGTCTC BsiHKAI 465 GTGCAC G[AT]GC[AT]^C 1795 GAGCTC BspHI 1574 TCATGA T^CATGA 1931 TCATGA BstAPI 933 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2145 GCAGACGCTGC BstXI 54 CCAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1811 CCATCGGCATGG Eco47III 493 AGCGCT AGC^GCT 2099 AGCGCT Hin1I 1367 GGCGCC G[GA]^CG[CT]C 1682 GGCGCC HincII 166 GTCAAC GT[CT]^[GA]AC 1945 GTCAAC HindII 166 GTCAAC GT[CT]^[GA]AC 1945 GTCAAC KasI 1367 GGCGCC G^GCGCC 1682 GGCGCC MaeI 754 CTAG C^TAG 1321 CTAG MaeIII 120 GTCAC ^GT[ACGT]AC 796 GTGAC MseI 380 TTAA T^TAA 1177 TTAA MslI 1440 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1812 CATCGGCATG NarI 1367 GGCGCC GG^CGCC 1682 GGCGCC NcoI 113 CCATGG C^CATGG 1730 CCATGG PflMI 656 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1472 CCATCGAGTGG PmaCI 1372 CACGTG CAC^GTG 1541 CACGTG PmlI 1372 CACGTG CAC^GTG 1541 CACGTG PpuMI 1598 GGGACCT [GA]G^G[AT]CC[CT] 2168 AGGACCC RcaI 1574 TCATGA T^CATGA 1931 TCATGA SapI 234 GCTCTTC GCTCTTC(1/4) 1797 GCTCTTC SbfI 711 CCTGCAGG CCTGCA^GG 873 CCTGCAGG SfoI 1367 GGCGCC GGC^GCC 1682 GGCGCC SmaI 251 CCCGGG CCC^GGG 572 CCCGGG SphI 567 GCATGC GCATG^C 747 GCATGC Sse8387I 711 CCTGCAGG CCTGCA^GG 873 CCTGCAGG Tru9I 380 TTAA T^TAA 1177 TTAA Tsp45I 120 GTCAC ^GT[GC]AC 796 GTGAC Tsp509I 146 AATT ^AATT 1805 AATT Van91I 656 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1472 CCATCGAGTGG XmaCI 251 CCCGGG C^CCGGG 572 CCCGGG XmaI 251 CCCGGG C^CCGGG 572 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI TspRI TthHB8I XhoII