(Circular) (Six-base) MAPSORT of: pFN21AE4577.seq Check: 4698 from: 1 to: 6997 pFN21AE4577 6997 bp With 182 enzymes: SgfI * December 3, 2014 10:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4046 4178 4046 Size: 132 6865 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4807 278 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4340 1937 Size: 2403 4594 Acc65I G'GTAC_C Cuts at: 3486 4318 3486 Size: 832 6165 AclI AA'CG_TT Cuts at: 1563 4296 5124 5497 1563 Size: 2733 828 373 3063 Fragments arranged by size: 3063 2733 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2918 3269 4099 5060 6444 6712 1632 Size: 1286 351 830 961 1384 268 1917 Fragments arranged by size: 1917 1384 1286 961 830 351 268 AfeI AGC'GCT Cuts at: 2500 4106 2500 Size: 1606 5391 AflII C'TTAA_G Cuts at: 820 1017 2384 820 Size: 197 1367 5433 Fragments arranged by size: 5433 1367 197 AflIII A'CryG_T Cuts at: 1203 3207 3378 3545 5896 6873 1203 Size: 2004 171 167 2351 977 1327 Fragments arranged by size: 2351 2004 1327 977 171 167 AgeI A'CCGG_T Cuts at: 5828 5828 Size: 6997 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 3449 1166 Size: 2283 4714 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3887 1184 Size: 2703 4294 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2114 Size: 32 6965 AlwNI CAG_nnn'CTG Cuts at: 2455 3439 3463 4156 4740 6312 2455 Size: 984 24 693 584 1572 3140 Fragments arranged by size: 3140 1572 984 693 584 24 ApaI G_GGCC'C Cuts at: 2839 3769 2839 Size: 930 6067 ApaLI G'TGCA_C Cuts at: 2470 5054 6210 2470 Size: 2584 1156 3257 Fragments arranged by size: 3257 2584 1156 ApoI r'AATT_y Cuts at: 1493 2150 3809 4306 4532 4684 6793 1493 Size: 657 1659 497 226 152 2109 1697 Fragments arranged by size: 2109 1697 1659 657 497 226 152 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 2256 2267 2577 4205 4322 1870 Size: 73 313 11 310 1628 117 4545 Fragments arranged by size: 4545 1628 313 310 117 73 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3763 3796 3763 Size: 33 6964 BamHI G'GATC_C Cuts at: 2212 4327 4694 2212 Size: 2115 367 4515 Fragments arranged by size: 4515 2115 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 3372 3486 3687 4250 Size: 325 217 627 411 1174 114 201 563 Cuts at: 4250 4318 5773 618 Size: 68 1455 1842 Fragments arranged by size: 1842 1455 1174 627 563 411 325 217 201 114 68 BanII G_rGCy'C Cuts at: 1391 2345 2823 2839 3098 3129 3769 3804 4316 Size: 954 478 16 259 31 640 35 512 Cuts at: 4316 1391 Size: 4072 Fragments arranged by size: 4072 954 640 512 478 259 35 31 16 BbeI G_GCGC'C Cuts at: 3376 3691 3376 Size: 315 6682 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3628 928 Size: 971 1729 4297 Fragments arranged by size: 4297 1729 971 BbvCI CC'TCA_GC Cuts at: 2448 2448 Size: 6997 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5188 5222 1903 Size: 34 3251 34 3678 Fragments arranged by size: 3678 3251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 4889 6099 3791 Size: 1098 1210 4689 Fragments arranged by size: 4689 1210 1098 BclI T'GATC_A Cuts at: 1548 1548 Size: 6997 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5608 136 Size: 107 122 71 631 605 3936 1525 Fragments arranged by size: 3936 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6992 6992 Size: 6997 BlpI GC'TnA_GC Cuts at: 4414 4414 Size: 6997 Bme1580I G_kGCm'C Cuts at: 1790 2474 2839 3769 4275 5058 6214 1790 Size: 684 365 930 506 783 1156 2573 Fragments arranged by size: 2573 1156 930 783 684 506 365 BmgBI CAC'GTC Cuts at: 1318 2358 1318 Size: 1040 5957 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 3763 4144 Size: 416 53 83 399 24 1044 1302 381 Cuts at: 4144 4728 5686 442 Size: 584 958 1753 Fragments arranged by size: 1753 1302 1044 958 584 416 399 381 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2192 2255 3076 2192 Size: 63 821 6113 Fragments arranged by size: 6113 821 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2002 2010 2711 3089 3994 4031 4271 5657 Size: 763 8 701 378 905 37 240 1386 Cuts at: 5657 1239 Size: 2579 Fragments arranged by size: 2579 1386 905 763 701 378 240 37 8 Bpu10I CC'TnA_GC Cuts at: 2297 2448 3671 2297 Size: 151 1223 5623 Fragments arranged by size: 5623 1223 151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2882 5122 5987 6285 2882 Size: 2240 865 298 3594 Fragments arranged by size: 3594 2240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2257 3938 5660 882 Size: 617 758 1681 1722 2219 Fragments arranged by size: 2219 1722 1681 758 617 BsaAI yAC'GTr Cuts at: 493 1204 3379 3548 3892 4788 493 Size: 711 2175 169 344 896 2702 Fragments arranged by size: 2702 2175 896 711 344 169 BsaBI GATnn'nnATC Cuts at: 6991 6991 Size: 6997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3373 3688 4804 5186 Size: 53 83 186 936 1840 315 1116 382 Cuts at: 5186 5821 275 Size: 635 1451 Fragments arranged by size: 1840 1451 1116 936 635 382 315 186 83 53 BsaWI w'CCGG_w Cuts at: 2145 5430 5828 6102 6249 6660 2145 Size: 3285 398 274 147 411 2482 Fragments arranged by size: 3285 2482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 6031 Fragments arranged by size: 6031 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2539 2813 3068 3332 4139 4274 1186 Size: 1353 274 255 264 807 135 3909 Fragments arranged by size: 3909 1353 807 274 264 255 135 BseYI C'CCAG_C Cuts at: 2831 3517 6200 6696 2831 Size: 686 2683 496 3132 Fragments arranged by size: 3132 2683 686 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 2876 3749 3836 4057 1163 Size: 573 753 387 873 87 221 4103 Fragments arranged by size: 4103 873 753 573 387 221 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 4447 5208 6236 1540 Size: 275 186 589 1857 761 1028 2301 Fragments arranged by size: 2301 1857 1028 761 589 275 186 BsiHKAI G_wGCw'C Cuts at: 2474 3804 5058 5143 6214 2474 Size: 1330 1254 85 1071 3257 Fragments arranged by size: 3257 1330 1254 1071 85 BsmI GAATG_Cn' Cuts at: 2406 4508 4601 2406 Size: 2102 93 4802 Fragments arranged by size: 4802 2102 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2345 2474 2823 2839 3098 3129 3769 Size: 399 555 129 349 16 259 31 640 Cuts at: 3769 3804 4275 4316 5058 5143 6214 1391 Size: 35 471 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 555 471 399 349 259 129 85 41 35 31 16 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 3579 3936 4886 6616 3579 Size: 357 950 1730 3960 Fragments arranged by size: 3960 1730 950 357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2886 3498 3540 3615 4046 4178 4352 844 Size: 2042 612 42 75 431 132 174 3489 Fragments arranged by size: 3489 2042 612 431 174 132 75 42 BsrBI CCG'CTC Cuts at: 1394 1823 2106 4884 1394 Size: 429 283 2778 3507 Fragments arranged by size: 3507 2778 429 283 BsrDI GCAATG_nn' Cuts at: 65 1248 2790 3075 3085 5492 5666 65 Size: 1183 1542 285 10 2407 174 1396 Fragments arranged by size: 2407 1542 1396 1183 285 174 10 BsrFI r'CCGG_y Cuts at: 1700 1810 5641 5828 1700 Size: 110 3831 187 2869 Fragments arranged by size: 3831 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6997 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6997 BssSI C'ACGA_G Cuts at: 3344 5057 6069 3344 Size: 1713 1012 4272 Fragments arranged by size: 4272 1713 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2944 4156 794 Size: 2150 1212 3635 Fragments arranged by size: 3635 2150 1212 BstBI TT'CG_AA Cuts at: 5801 5801 Size: 6997 BstEII G'GTnAC_C Cuts at: 1184 2800 1184 Size: 1616 5381 BstXI CCAn_nnnn'nTGG Cuts at: 2066 3823 2066 Size: 1757 5240 BstYI r'GATC_y Cuts at: 1972 2212 2272 2884 3157 3333 3390 4327 4694 Size: 240 60 612 273 176 57 937 367 Cuts at: 4694 5079 5096 6548 6634 6646 6992 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 937 612 385 367 346 273 240 176 86 60 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 3735 3859 513 Size: 552 1053 1617 124 3651 Fragments arranged by size: 3651 1617 1053 552 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2494 3913 487 Size: 683 56 1268 1419 3571 Fragments arranged by size: 3571 1419 1268 683 56 BtsI GCAGTG_nn' Cuts at: 2414 2540 2672 4513 5307 5327 2414 Size: 126 132 1841 794 20 4084 Fragments arranged by size: 4084 1841 794 132 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6997 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6962 DraI TTT'AAA Cuts at: 4301 4301 Size: 6997 DraIII CAC_nnn'GTG Cuts at: 2994 2994 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6004 809 Size: 507 4688 1802 Fragments arranged by size: 4688 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2587 2649 2904 Size: 54 1425 50 275 18 757 62 255 Cuts at: 2904 3474 4444 5333 6962 8 Size: 570 970 889 1629 43 Fragments arranged by size: 1629 1425 970 889 757 570 275 255 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2587 4444 1537 Size: 275 775 1857 4090 Fragments arranged by size: 4090 1857 775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 2516 3428 3809 4927 1353 Size: 893 270 912 381 1118 3423 Fragments arranged by size: 3423 1118 912 893 381 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 5584 5958 6104 387 Size: 1064 138 504 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 504 374 146 138 EcoICRI GAG'CTC Cuts at: 3802 3802 Size: 6997 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2010 2711 2918 3089 3269 3994 Size: 393 370 8 701 207 171 180 725 Cuts at: 3994 4031 4099 4271 5060 5657 6444 6712 1239 Size: 37 68 172 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 725 701 597 393 370 268 207 180 172 171 68 37 8 EcoNI CCTnn'n_nnAGG Cuts at: 2366 3431 3674 2366 Size: 1065 243 5689 Fragments arranged by size: 5689 1065 243 EcoO109I rG'GnC_Cy Cuts at: 2835 3436 3604 3707 3765 4174 2835 Size: 601 168 103 58 409 5658 Fragments arranged by size: 5658 601 409 168 103 58 EcoRI G'AATT_C Cuts at: 4306 4684 4306 Size: 378 6619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3196 3196 Size: 6997 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 2502 3376 3691 4108 6144 1678 Size: 824 874 315 417 2036 2531 Fragments arranged by size: 2531 2036 874 824 417 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 4163 4195 4238 Size: 32 135 32 848 32 1711 32 43 Cuts at: 4238 4270 5638 5670 5712 5744 1373 Size: 32 1368 32 42 32 2626 Fragments arranged by size: 2626 1711 1368 848 135 43 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 3952 4341 4587 669 Size: 988 281 235 1779 389 246 3079 Fragments arranged by size: 3079 1779 988 389 281 246 235 HindIII A'AGCT_T Cuts at: 748 748 Size: 6997 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KasI G'GCGC_C Cuts at: 3372 3687 3372 Size: 315 6682 KpnI G_GTAC'C Cuts at: 3490 4322 3490 Size: 832 6165 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 3265 4317 6111 6295 1305 Size: 714 1246 1052 1794 184 2007 Fragments arranged by size: 2007 1794 1246 1052 714 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3476 10 Size: 54 1425 343 1644 3531 Fragments arranged by size: 3531 1644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3449 3821 4955 5314 5473 518 Size: 648 2283 372 1134 359 159 2042 Fragments arranged by size: 2283 2042 1134 648 372 359 159 MspA1I CmG'CkG Cuts at: 1606 2783 2950 3048 3224 3355 3850 3861 3972 Size: 1177 167 98 176 131 495 11 111 Cuts at: 3972 4051 4413 5090 6238 6483 1606 Size: 79 362 677 1148 245 2120 Fragments arranged by size: 2120 1177 1148 677 495 362 245 176 167 131 111 98 79 11 NarI GG'CG_CC Cuts at: 3373 3688 3373 Size: 315 6682 NcoI C'CATG_G Cuts at: 513 1065 2118 3735 513 Size: 552 1053 1617 3775 Fragments arranged by size: 3775 1617 1053 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6997 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 2587 4444 2587 Size: 1857 5140 NspI r_CATG'y Cuts at: 1132 1229 2576 2756 2812 3211 3925 4355 5900 Size: 97 1347 180 56 399 714 430 1545 Cuts at: 5900 1132 Size: 2229 Fragments arranged by size: 2229 1545 1347 714 430 399 180 97 56 PasI CC'CwG_GG Cuts at: 1348 2734 1348 Size: 1386 5611 PciI A'CATG_T Cuts at: 3207 5896 3207 Size: 2689 4308 PflMI CCAn_nnn'nTGG Cuts at: 1108 2667 3483 1108 Size: 1559 816 4622 Fragments arranged by size: 4622 1559 816 PfoI T'CCnGG_A Cuts at: 2688 3783 3840 4008 2688 Size: 1095 57 168 5677 Fragments arranged by size: 5677 1095 168 57 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PmlI CAC'GTG Cuts at: 3379 3548 3379 Size: 169 6828 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2114 2146 5066 5098 6579 6611 2114 Size: 32 2920 32 1481 32 2500 Fragments arranged by size: 2920 2500 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3604 4174 3604 Size: 570 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PspOMI G'GGCC_C Cuts at: 2835 3765 2835 Size: 930 6067 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3521 3553 3521 Size: 32 6965 PstI C_TGCA'G Cuts at: 830 2424 2535 2721 2883 2949 3147 3993 4349 Size: 1594 111 186 162 66 198 846 356 Cuts at: 4349 830 Size: 3478 Fragments arranged by size: 3478 1594 846 356 198 186 162 111 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 PvuII CAG'CTG Cuts at: 2783 3048 3355 3850 3972 2783 Size: 265 307 495 122 5808 Fragments arranged by size: 5808 495 307 265 122 SacI G_AGCT'C Cuts at: 3804 3804 Size: 6997 SacII CC_GC'GG Cuts at: 3862 3862 Size: 6997 SalI G'TCGA_C Cuts at: 1936 4339 1936 Size: 2403 4594 SapI GCTCTTCn'nnn_ Cuts at: 2246 3809 2246 Size: 1563 5434 SbfI CC_TGCA'GG Cuts at: 2721 2883 4349 2721 Size: 162 1466 5369 Fragments arranged by size: 5369 1466 162 ScaI AGT'ACT Cuts at: 5245 6957 5245 Size: 1712 5285 SfcI C'TryA_G Cuts at: 826 1045 2420 2531 2717 2879 2945 3143 3571 Size: 219 1375 111 186 162 66 198 428 Cuts at: 3571 3654 3702 3989 4345 5480 6161 6352 826 Size: 83 48 287 356 1135 681 191 1471 Fragments arranged by size: 1471 1375 1135 681 428 356 287 219 198 191 186 162 111 83 66 48 SfoI GGC'GCC Cuts at: 3374 3689 3374 Size: 315 6682 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 2258 2579 4324 1872 Size: 386 321 1745 4545 Fragments arranged by size: 4545 1745 386 321 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 2861 4205 5101 6002 6264 Size: 197 926 441 477 1344 896 901 262 Cuts at: 6264 820 Size: 1553 Fragments arranged by size: 1553 1344 926 901 896 477 441 262 197 SnaBI TAC'GTA Cuts at: 493 4788 493 Size: 4295 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 2576 2756 4355 1132 Size: 1444 180 1599 3774 Fragments arranged by size: 3774 1599 1444 180 SrfI GCCC'GGGC Cuts at: 2579 2579 Size: 6997 SspI AAT'ATT Cuts at: 5 52 4921 5 Size: 47 4869 2081 Fragments arranged by size: 4869 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6997 StyI C'CwwG_G Cuts at: 513 1065 2118 2608 2910 3735 3795 4233 4436 Size: 552 1053 490 302 825 60 438 203 Cuts at: 4436 513 Size: 3074 Fragments arranged by size: 3074 1053 825 552 490 438 302 203 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2244 5024 5041 5194 5379 5824 325 Size: 1255 664 2780 17 153 185 445 1498 Fragments arranged by size: 2780 1498 1255 664 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3771 5243 6955 Size: 274 80 33 51 1443 1794 1472 1712 Cuts at: 6955 96 Size: 138 Fragments arranged by size: 1794 1712 1472 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3537 3569 6957 6989 3537 Size: 32 3388 32 3545 Fragments arranged by size: 3545 3388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2393 1316 Size: 1077 5920 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4006 4006 Size: 6997 XhoI C'TCGA_G Cuts at: 1943 4205 1943 Size: 2262 4735 XmaI C'CCGG_G Cuts at: 1870 2256 2577 4322 1870 Size: 386 321 1745 4545 Fragments arranged by size: 4545 1745 386 321 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4805 276 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SanDI SexAI SfiI SwaI