(Circular) (Six-base) MAPSORT of: pFN21AE3923.seq Check: 5704 from: 1 to: 6046 pFN21AE3923 6046 bp With 182 enzymes: SgfI * March 28, 2012 11:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3163 3163 Size: 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2422 2521 3076 3389 1937 Size: 485 99 555 313 4594 Fragments arranged by size: 4594 555 485 313 99 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2684 4109 5493 5761 1632 Size: 1052 1425 1384 268 1917 Fragments arranged by size: 1917 1425 1384 1052 268 AfeI AGC'GCT Cuts at: 2345 2345 Size: 6046 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 2368 2773 4945 5922 1203 Size: 1165 405 2172 977 1327 Fragments arranged by size: 2172 1327 1165 977 405 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2363 1184 Size: 1179 4867 AlwNI CAG_nnn'CTG Cuts at: 3789 5361 3789 Size: 1572 4474 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 2506 2686 2927 3086 3331 3355 3581 3733 Size: 1013 180 241 159 245 24 226 152 Cuts at: 3733 5842 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1013 245 241 226 180 159 152 24 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 3371 1870 Size: 73 1428 4545 Fragments arranged by size: 4545 1428 73 AvrII C'CTAG_G Cuts at: 2312 2312 Size: 6046 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 2088 3367 4822 618 Size: 325 217 627 226 75 1279 1455 1842 Fragments arranged by size: 1842 1455 1279 627 325 226 217 75 BanII G_rGCy'C Cuts at: 1391 2312 3365 1391 Size: 921 1053 4072 Fragments arranged by size: 4072 1053 921 BbeI G_GCGC'C Cuts at: 2017 2092 2017 Size: 75 5971 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2218 2439 928 Size: 971 319 221 4535 Fragments arranged by size: 4535 971 319 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 3052 1548 Size: 1504 4542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 4107 5263 1790 Size: 2317 1156 2573 Fragments arranged by size: 2573 2317 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 4735 442 Size: 416 53 83 399 24 2360 958 1753 Fragments arranged by size: 2360 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 4706 1239 Size: 1280 2187 2579 Fragments arranged by size: 2579 2187 1280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 4709 882 Size: 617 909 2301 2219 Fragments arranged by size: 2301 2219 909 617 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 2089 3853 4235 Size: 53 83 186 936 481 75 1764 382 Cuts at: 4235 4870 275 Size: 635 1451 Fragments arranged by size: 1764 1451 936 635 481 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 2177 4479 4877 5151 5298 5709 2177 Size: 2302 398 274 147 411 2514 Fragments arranged by size: 2514 2302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 3266 1186 Size: 1330 750 3966 Fragments arranged by size: 3966 1330 750 BseYI C'CCAG_C Cuts at: 2066 5249 5745 2066 Size: 3183 496 2367 Fragments arranged by size: 3183 2367 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2840 4107 4192 5263 2840 Size: 1267 85 1071 3623 Fragments arranged by size: 3623 1267 1071 85 BsmI GAATG_Cn' Cuts at: 2632 2876 3557 3650 2632 Size: 244 681 93 5028 Fragments arranged by size: 5028 681 244 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2840 3365 4107 4192 5263 1391 Size: 399 522 528 525 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 525 522 399 85 BspEI T'CCGG_A Cuts at: 2177 5709 2177 Size: 3532 2514 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3163 3197 3401 844 Size: 2319 34 204 3489 Fragments arranged by size: 3489 2319 204 34 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 4690 4877 1700 Size: 110 200 2680 187 2869 Fragments arranged by size: 2869 2680 200 187 110 BsrGI T'GTAC_A Cuts at: 96 2886 96 Size: 2790 3256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 2758 4850 2758 Size: 2092 3954 BstEII G'GTnAC_C Cuts at: 1184 2295 1184 Size: 1111 4935 BstYI r'GATC_y Cuts at: 1972 2853 3376 3743 4128 4145 5597 5683 5695 Size: 881 523 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 881 523 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5494 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 2079 3562 4356 4376 2079 Size: 1483 794 20 3749 Fragments arranged by size: 3749 1483 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 3493 4382 Size: 54 1425 50 275 18 206 1457 889 Cuts at: 4382 6011 8 Size: 1629 43 Fragments arranged by size: 1629 1457 1425 889 275 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3976 1353 Size: 2623 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2684 4109 4706 5493 5761 1239 Size: 393 887 165 1425 597 787 268 1524 Fragments arranged by size: 1524 1425 887 787 597 393 268 165 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2498 Size: 6046 EcoO109I rG'GnC_Cy Cuts at: 3299 3299 Size: 6046 EcoRI G'AATT_C Cuts at: 3086 3355 3733 3086 Size: 269 378 5399 Fragments arranged by size: 5399 378 269 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 2017 2061 2092 2347 5193 1678 Size: 339 44 31 255 2846 2531 Fragments arranged by size: 2846 2531 339 255 44 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373 Size: 32 135 32 3115 32 42 32 2626 Fragments arranged by size: 3115 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3077 3390 3636 669 Size: 988 281 1139 313 246 3079 Fragments arranged by size: 3079 1139 988 313 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KasI G'GCGC_C Cuts at: 2013 2088 2013 Size: 75 5971 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5160 5344 1305 Size: 3855 184 2007 Fragments arranged by size: 3855 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4224 Fragments arranged by size: 4224 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4004 4363 4522 518 Size: 648 2838 359 159 2042 Fragments arranged by size: 2838 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2046 3462 4139 5287 5532 1606 Size: 419 21 1416 677 1148 245 2120 Fragments arranged by size: 2120 1416 1148 677 419 245 21 NaeI GCC'GGC Cuts at: 2012 2012 Size: 6046 NarI GG'CG_CC Cuts at: 2014 2089 2014 Size: 75 5971 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5494 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 2010 2010 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 2372 2777 3404 4949 1132 Size: 97 1143 405 627 1545 2229 Fragments arranged by size: 2229 1545 1143 627 405 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6046 PciI A'CATG_T Cuts at: 2368 2773 4945 2368 Size: 405 2172 3469 Fragments arranged by size: 3469 2172 405 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PfoI T'CCnGG_A Cuts at: 2496 2496 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3299 3299 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PstI C_TGCA'G Cuts at: 830 2024 2057 2700 3398 830 Size: 1194 33 643 698 3478 Fragments arranged by size: 3478 1194 698 643 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2025 2025 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3075 3388 1936 Size: 1139 313 4594 Fragments arranged by size: 4594 1139 313 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2020 2053 2696 3394 4529 5210 5401 Size: 219 975 33 643 698 1135 681 191 Cuts at: 5401 826 Size: 1471 Fragments arranged by size: 1471 1135 975 698 681 643 219 191 33 SfoI GGC'GCC Cuts at: 2015 2090 2015 Size: 75 5971 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 2215 152 Size: 2063 3983 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6046 StyI C'CwwG_G Cuts at: 513 1065 2312 3485 513 Size: 552 1247 1173 3074 Fragments arranged by size: 3074 1247 1173 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2390 2886 4292 Size: 274 80 33 51 1443 413 496 1406 Cuts at: 4292 6004 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1406 496 413 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 2754 4175 2754 Size: 1421 4625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI