################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 463 GTAGAC GT^[AC][GT]AC Alw44I 1546 GTGCAC G^TGCAC AlwI 234 GGATC GGATC(4/5) AocI 177 CCTGAGG CC^T[ACGT]AGG Aor51HI 1145 AGCGCT AGC^GCT ApaLI 1546 GTGCAC G^TGCAC Asp700I 447 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 347 TGGCCA TGG^CCA BbeI 1508 GGCGCC GGCGC^C BlpI 1452 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1452 GCTGAGC GC^T[ACGT]AGC BsaI 868 GGTCTC GGTCTC(1/5) BsmBI 745 CGTCTC CGTCTC(1/5) BstAPI 1368 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 234 GGATCT [GA]^GATC[CT] Bsu36I 177 CCTGAGG CC^T[ACGT]AGG CelII 1452 GCTGAGC GC^T[ACGT]AGC DraIII 517 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1145 AGCGCT AGC^GCT Eco81I 177 CCTGAGG CC^T[ACGT]AGG EcoNI 388 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 757 ATGCAT ATGCA^T KasI 1508 GGCGCC G^GCGCC KspI 788 CCGCGG CCGC^GG MflI 234 GGATCT [GA]^GATC[CT] MluNI 347 TGGCCA TGG^CCA MscI 347 TGGCCA TGG^CCA NarI 1508 GGCGCC GG^CGCC NsiI 757 ATGCAT ATGCA^T NspI 289 ACATGC [GA]CATG^[CT] PflFI 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 382 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1533 GACTC GAGTC(4/5) PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT] SacI 1431 GAGCTC GAGCT^C SacII 788 CCGCGG CCGC^GG SfoI 1508 GGCGCC GGC^GCC Tth111I 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 382 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 237 TCTAGA T^CTAGA XcmI 335 CCAACCTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 234 GGATCT [GA]^GATC[CT] XmnI 447 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 743 GGCGTC G[GA]^CG[CT]C 1508 GGCGCC AlwNI 1369 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1444 CAGACGCTG ApaI 1175 GGGCCC GGGCC^C 1284 GGGCCC BbrPI 574 CACGTG CAC^GTG 1543 CACGTG BbsI 10 GAAGAC GAAGAC(2/6) 136 GAAGAC BpuAI 10 GAAGAC GAAGAC(2/6) 136 GAAGAC BsaAI 574 CACGTG [CT]AC^GT[GA] 1543 CACGTG BsaHI 743 GGCGTC G[GA]^CG[CT]C 1508 GGCGCC BsgI 544 GTGCAG GTGCAG(16/14) 607 GTGCAG BsiEI 1154 CGGCCG CG[GA][CT]^CG 1194 CGGCCG BsrFI 1196 GCCGGC [GA]^CCGG[CT] 1349 GCCGGC BstXI 476 CCATCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 934 CCAGAGGTCTGG Cfr10I 1196 GCCGGC [GA]^CCGG[CT] 1349 GCCGGC DpnII 235 GATC ^GATC 412 GATC DsaI 788 CCGCGG C^C[GA][CT]GG 1301 CCGTGG EagI 1154 CGGCCG C^GGCCG 1194 CGGCCG EclXI 1154 CGGCCG C^GGCCG 1194 CGGCCG Eco52I 1154 CGGCCG C^GGCCG 1194 CGGCCG Hin1I 743 GGCGTC G[GA]^CG[CT]C 1508 GGCGCC MaeII 575 ACGT A^CGT 1544 ACGT MboI 235 GATC ^GATC 412 GATC MslI 477 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 967 CATAGCCATG NaeI 1196 GCCGGC GCC^GGC 1349 GCCGGC NdeII 235 GATC ^GATC 412 GATC NgoMIV 1196 GCCGGC G^CCGGC 1349 GCCGGC PmaCI 574 CACGTG CAC^GTG 1543 CACGTG PmlI 574 CACGTG CAC^GTG 1543 CACGTG Sau3AI 235 GATC ^GATC 412 GATC TaqI 41 TCGA T^CGA 393 TCGA TthHB8I 41 TCGA T^CGA 393 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI PvuII RsaI SapI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI