(Circular) (Six-base) MAPSORT of: pFN21AE3931.seq Check: 518 from: 1 to: 6340 pFN21AE3931 6340 bp With 182 enzymes: SgfI * March 28, 2012 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3372 3372 Size: 6340 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2469 3683 1937 Size: 532 1214 4594 Fragments arranged by size: 4594 1214 532 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 2675 2745 4403 5787 6055 1632 Size: 839 204 70 1658 1384 268 1917 Fragments arranged by size: 1917 1658 1384 839 268 204 70 AfeI AGC'GCT Cuts at: 3152 3152 Size: 6340 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 5239 6216 1203 Size: 4036 977 1327 Fragments arranged by size: 4036 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3150 1184 Size: 1966 4374 AlwNI CAG_nnn'CTG Cuts at: 3379 3454 4083 5655 3379 Size: 75 629 1572 4064 Fragments arranged by size: 4064 1572 629 75 ApaI G_GGCC'C Cuts at: 3184 3293 3184 Size: 109 6231 ApaLI G'TGCA_C Cuts at: 3551 4397 5553 3551 Size: 846 1156 4338 Fragments arranged by size: 4338 1156 846 ApoI r'AATT_y Cuts at: 1493 3649 3875 4027 6136 1493 Size: 2156 226 152 2109 1697 Fragments arranged by size: 2156 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 3665 1870 Size: 73 1722 4545 Fragments arranged by size: 4545 1722 73 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2530 3407 3513 3617 3661 Size: 325 217 627 743 877 106 104 44 Cuts at: 3661 5116 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 877 743 627 325 217 106 104 44 BanII G_rGCy'C Cuts at: 1391 3184 3293 3440 3464 3659 1391 Size: 1793 109 147 24 195 4072 Fragments arranged by size: 4072 1793 195 147 109 24 BbeI G_GCGC'C Cuts at: 3517 3517 Size: 6340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 2148 928 Size: 971 123 126 5120 Fragments arranged by size: 5120 971 126 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4531 4565 1903 Size: 34 50 34 2510 34 3678 Fragments arranged by size: 3678 2510 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BfrBI ATG'CAT Cuts at: 2764 2764 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4951 136 Size: 107 122 71 631 605 3279 1525 Fragments arranged by size: 3279 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3458 3757 3458 Size: 299 6041 Bme1580I G_kGCm'C Cuts at: 1790 2580 3184 3293 3410 3555 3620 4401 5557 Size: 790 604 109 117 145 65 781 1156 Cuts at: 5557 1790 Size: 2573 Fragments arranged by size: 2573 1156 790 781 604 145 117 109 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3185 3220 3459 Size: 416 53 83 399 24 1768 35 239 Cuts at: 3459 4071 5029 442 Size: 612 958 1753 Fragments arranged by size: 1768 1753 958 612 416 399 239 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2337 2337 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2256 2624 2870 3368 5000 1239 Size: 1001 16 368 246 498 1632 2579 Fragments arranged by size: 2579 1632 1001 498 368 246 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5330 5628 4465 Size: 865 298 5177 Fragments arranged by size: 5177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 5003 882 Size: 617 1380 2124 2219 Fragments arranged by size: 2219 2124 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 2581 3550 4131 493 Size: 711 1377 969 581 2702 Fragments arranged by size: 2702 1377 969 711 581 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2749 3514 4147 4529 Size: 53 83 186 936 1216 765 633 382 Cuts at: 4529 5164 275 Size: 635 1451 Fragments arranged by size: 1451 1216 936 765 635 633 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6115 Fragments arranged by size: 6115 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2063 2138 2141 2150 2186 2189 Size: 865 3 9 75 3 9 36 3 Cuts at: 2189 2213 2222 2237 2555 2648 3319 1186 Size: 24 9 15 318 93 671 4207 Fragments arranged by size: 4207 865 671 318 93 75 36 24 15 9 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 2097 2772 2776 2823 3240 3369 3509 5543 6039 Size: 675 4 47 417 129 140 2034 496 Cuts at: 6039 2097 Size: 2398 Fragments arranged by size: 2398 2034 675 496 417 140 129 47 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 2633 1163 Size: 573 834 63 4870 Fragments arranged by size: 4870 834 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3162 3202 3790 4551 5579 1540 Size: 275 186 1161 40 588 761 1028 2301 Fragments arranged by size: 2301 1161 1028 761 588 275 186 40 BsiHKAI G_wGCw'C Cuts at: 3440 3555 3609 4401 4486 5557 3440 Size: 115 54 792 85 1071 4223 Fragments arranged by size: 4223 1071 792 115 85 54 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 BsmBI CGTCTCn'nnnn_ Cuts at: 2756 2756 Size: 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2580 3184 3293 3410 3440 3464 3555 Size: 399 790 604 109 117 30 24 91 Cuts at: 3555 3609 3620 3659 4401 4486 5557 1391 Size: 54 11 39 742 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 604 399 117 109 91 85 54 39 30 24 11 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2352 3372 3695 844 Size: 1235 273 1020 323 3489 Fragments arranged by size: 3489 1235 1020 323 273 BsrBI CCG'CTC Cuts at: 1394 1823 2857 3121 3195 4227 1394 Size: 429 1034 264 74 1032 3507 Fragments arranged by size: 3507 1034 1032 429 264 74 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3201 3354 4984 5171 1700 Size: 110 1391 153 1630 187 2869 Fragments arranged by size: 2869 1630 1391 187 153 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 3379 794 Size: 2585 3755 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6340 BstXI CCAn_nnnn'nTGG Cuts at: 2488 2946 2488 Size: 458 5882 BstYI r'GATC_y Cuts at: 1972 2239 3670 4037 4422 4439 5891 5977 5989 Size: 267 1431 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1431 385 367 346 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2183 2183 Size: 6340 BtgI C'CryG_G Cuts at: 513 1065 2793 3306 513 Size: 552 1728 513 3547 Fragments arranged by size: 3547 1728 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3329 487 Size: 683 56 2103 3498 Fragments arranged by size: 3498 2103 683 56 BtsI GCAGTG_nn' Cuts at: 2258 2616 3856 4650 4670 2258 Size: 358 1240 794 20 3928 Fragments arranged by size: 3928 1240 794 358 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6305 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DraIII CAC_nnn'GTG Cuts at: 2527 2527 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 3159 3199 Size: 54 1425 50 275 18 522 807 40 Cuts at: 3199 3787 4676 6305 8 Size: 588 889 1629 43 Fragments arranged by size: 1629 1425 889 807 588 522 275 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3159 3199 3787 1537 Size: 275 1347 40 588 4090 Fragments arranged by size: 4090 1347 588 275 40 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2229 2462 2469 2651 2721 2913 4270 Size: 699 177 233 7 182 70 192 1357 Cuts at: 4270 1353 Size: 3423 Fragments arranged by size: 3423 1357 699 233 192 182 177 70 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2324 2341 3098 4927 5301 5447 Size: 1064 138 735 17 757 1829 374 146 Cuts at: 5447 387 Size: 1280 Fragments arranged by size: 1829 1280 1064 757 735 374 146 138 17 EcoICRI GAG'CTC Cuts at: 3438 3438 Size: 6340 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2256 2471 2624 2675 2745 2870 Size: 393 608 16 215 153 51 70 125 Cuts at: 2870 3368 4403 5000 5787 6055 1239 Size: 498 1035 597 787 268 1524 Fragments arranged by size: 1524 1035 787 608 597 498 393 268 215 153 125 70 51 16 EcoNI CCTnn'n_nnAGG Cuts at: 2397 2397 Size: 6340 EcoO109I rG'GnC_Cy Cuts at: 2179 3180 3290 2179 Size: 1001 110 5229 Fragments arranged by size: 5229 1001 110 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 3154 3484 3517 5487 1678 Size: 1476 330 33 1970 2531 Fragments arranged by size: 2531 1970 1476 330 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2933 2965 3010 3042 3311 Size: 32 135 32 1361 32 45 32 269 Cuts at: 3311 3343 4981 5013 5055 5087 1373 Size: 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 1361 269 135 45 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KasI G'GCGC_C Cuts at: 3513 3513 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2354 10 Size: 54 1425 343 522 3996 Fragments arranged by size: 3996 1425 522 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 2976 4298 4657 4816 518 Size: 648 1320 490 1322 359 159 2042 Fragments arranged by size: 2042 1322 1320 648 490 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2350 2684 2776 2795 2929 3315 3388 Size: 491 253 334 92 19 134 386 73 Cuts at: 3388 3756 4433 5581 5826 1606 Size: 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 491 386 368 334 253 245 134 92 73 19 NaeI GCC'GGC Cuts at: 3203 3356 3203 Size: 153 6187 NarI GG'CG_CC Cuts at: 3514 3514 Size: 6340 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5788 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NgoMIV G'CCGG_C Cuts at: 3201 3354 3201 Size: 153 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NsiI A_TGCA'T Cuts at: 2766 2766 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2298 3698 5243 1132 Size: 97 1069 1400 1545 2229 Fragments arranged by size: 2229 1545 1400 1069 97 PasI CC'CwG_GG Cuts at: 1348 3184 3293 1348 Size: 1836 109 4395 Fragments arranged by size: 4395 1836 109 PciI A'CATG_T Cuts at: 5239 5239 Size: 6340 PflMI CCAn_nnn'nTGG Cuts at: 1108 2393 1108 Size: 1285 5055 PfoI T'CCnGG_A Cuts at: 2504 2886 2504 Size: 382 5958 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2581 3550 2581 Size: 969 5371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2051 2083 2736 2768 4409 4441 5922 5954 2051 Size: 32 653 32 1641 32 1481 32 2437 Fragments arranged by size: 2437 1641 1481 653 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2179 Size: 6340 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 3180 3289 3180 Size: 109 6231 PstI C_TGCA'G Cuts at: 830 2349 2574 2934 3692 830 Size: 1519 225 360 758 3478 Fragments arranged by size: 3478 1519 758 360 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2097 2350 2776 2929 2097 Size: 253 426 153 5508 Fragments arranged by size: 5508 426 253 153 SacI G_AGCT'C Cuts at: 3440 3440 Size: 6340 SacII CC_GC'GG Cuts at: 2796 2796 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SapI GCTCTTCn'nnn_ Cuts at: 2462 2651 2721 2462 Size: 189 70 6081 Fragments arranged by size: 6081 189 70 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2345 2570 2930 3688 4823 5504 5695 Size: 219 1300 225 360 758 1135 681 191 Cuts at: 5695 826 Size: 1471 Fragments arranged by size: 1471 1300 1135 758 681 360 225 219 191 SfoI GGC'GCC Cuts at: 3515 3515 Size: 6340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4444 5345 5607 820 Size: 197 926 2501 901 262 1553 Fragments arranged by size: 2501 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2628 3476 3612 3779 513 Size: 552 1563 848 136 167 3074 Fragments arranged by size: 3074 1563 848 552 167 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2400 2429 3239 4367 4384 4537 4722 Size: 1255 820 29 810 1128 17 153 185 Cuts at: 4722 5167 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1128 820 810 445 185 153 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 4586 6298 Size: 274 80 33 51 1443 579 2030 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 2030 1712 1443 579 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 3383 3415 6300 6332 2102 Size: 32 1249 32 2885 32 2110 Fragments arranged by size: 2885 2110 1249 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2249 1316 Size: 933 5407 XbaI T'CTAG_A Cuts at: 2242 3676 2242 Size: 1434 4906 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2347 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 2456 4469 2456 Size: 2013 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI