(Circular) (Six-base) MAPSORT of: pFN21AB5326.seq Check: 6653 from: 1 to: 6055 pFN21AB5326 6055 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 3398 1937 Size: 202 1259 4594 Fragments arranged by size: 4594 1259 202 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 4118 5502 5770 1632 Size: 986 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 986 268 AfeI AGC'GCT Cuts at: 2622 2622 Size: 6055 AflII C'TTAA_G Cuts at: 820 1017 2672 820 Size: 197 1655 4203 Fragments arranged by size: 4203 1655 197 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2682 1166 Size: 1516 4539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 2078 3798 5370 2078 Size: 1720 1572 2763 Fragments arranged by size: 2763 1720 1572 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2330 2408 3197 3312 3364 3590 3742 5851 Size: 837 78 789 115 52 226 152 2109 Cuts at: 5851 1493 Size: 1697 Fragments arranged by size: 2109 1697 837 789 226 152 115 78 52 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2027 3380 1870 Size: 73 84 1353 4545 Fragments arranged by size: 4545 1353 84 73 BamHI G'GATC_C Cuts at: 2443 3385 3752 2443 Size: 942 367 4746 Fragments arranged by size: 4746 942 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2047 3376 4831 618 Size: 325 217 627 227 33 1329 1455 1842 Fragments arranged by size: 1842 1455 1329 627 325 227 217 33 BanII G_rGCy'C Cuts at: 1391 2069 2132 3374 1391 Size: 678 63 1242 4072 Fragments arranged by size: 4072 1242 678 63 BbeI G_GCGC'C Cuts at: 2051 2051 Size: 6055 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4246 4280 1903 Size: 34 2309 34 3678 Fragments arranged by size: 3678 2309 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BfrBI ATG'CAT Cuts at: 2864 2864 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 4116 5272 1790 Size: 2326 1156 2573 Fragments arranged by size: 2573 2326 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2595 3054 3786 Size: 416 53 83 399 24 1178 459 732 Cuts at: 3786 4744 442 Size: 958 1753 Fragments arranged by size: 1753 1178 958 732 459 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2593 3090 4715 1239 Size: 785 569 497 1625 2579 Fragments arranged by size: 2579 1625 785 569 497 Bpu10I CC'TnA_GC Cuts at: 3000 3333 3000 Size: 333 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2512 4180 5045 5343 2512 Size: 1668 865 298 3224 Fragments arranged by size: 3224 1668 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4718 882 Size: 617 3219 2219 Fragments arranged by size: 3219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 2180 6049 2180 Size: 3869 2186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 3862 4244 4879 Size: 53 83 186 936 515 1814 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1814 1451 936 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2020 4488 4886 5160 5307 5718 2020 Size: 2468 398 274 147 411 2357 Fragments arranged by size: 2468 2357 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6055 BseYI C'CCAG_C Cuts at: 2062 2078 2083 5258 5754 2062 Size: 16 5 3175 496 2363 Fragments arranged by size: 3175 2363 496 16 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2977 1163 Size: 573 1241 4241 Fragments arranged by size: 4241 1241 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 3505 4266 5294 1540 Size: 275 186 96 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 96 BsiHKAI G_wGCw'C Cuts at: 2132 4116 4201 5272 2132 Size: 1984 85 1071 2915 Fragments arranged by size: 2915 1984 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3566 3659 3344 Size: 222 93 5740 Fragments arranged by size: 5740 222 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2132 3374 4116 4201 5272 1391 Size: 399 279 63 1242 742 85 1071 2174 Fragments arranged by size: 2174 1242 1071 742 399 279 85 63 BspEI T'CCGG_A Cuts at: 2020 5718 2020 Size: 3698 2357 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3410 844 Size: 2566 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2032 3942 1394 Size: 429 209 1910 3507 Fragments arranged by size: 3507 1910 429 209 BsrDI GCAATG_nn' Cuts at: 65 1248 2942 4550 4724 65 Size: 1183 1694 1608 174 1396 Fragments arranged by size: 1694 1608 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4699 4886 1700 Size: 110 234 2655 187 2869 Fragments arranged by size: 2869 2655 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstYI r'GATC_y Cuts at: 1972 2443 3385 3752 4137 4154 5606 5692 5704 Size: 471 942 367 385 17 1452 86 12 Cuts at: 5704 6050 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 942 471 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 3032 3571 4365 4385 3032 Size: 539 794 20 4702 Fragments arranged by size: 4702 794 539 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3502 4391 6020 Size: 54 1425 50 275 18 1672 889 1629 Cuts at: 6020 8 Size: 43 Fragments arranged by size: 1672 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 3985 1353 Size: 765 1867 3423 Fragments arranged by size: 3423 1867 765 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2130 2130 Size: 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2593 2618 3090 4118 4715 5502 Size: 393 392 569 25 472 1028 597 787 Cuts at: 5502 5770 1239 Size: 268 1524 Fragments arranged by size: 1524 1028 787 597 569 472 393 392 268 25 EcoO109I rG'GnC_Cy Cuts at: 3265 3265 Size: 6055 EcoRI G'AATT_C Cuts at: 3312 3364 3742 3312 Size: 52 378 5625 Fragments arranged by size: 5625 378 52 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2942 3358 2942 Size: 416 5639 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2051 2624 5202 1678 Size: 373 573 2578 2531 Fragments arranged by size: 2578 2531 573 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2514 2546 2827 2859 2983 Size: 32 135 32 942 32 281 32 124 Cuts at: 2983 2996 3015 3028 4696 4728 4770 4802 1373 Size: 13 19 13 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 942 281 135 124 42 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2140 3399 3645 669 Size: 988 281 202 1259 246 3079 Fragments arranged by size: 3079 1259 988 281 246 202 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2047 2047 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4233 Fragments arranged by size: 4233 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2283 2682 2853 4013 4372 4531 Size: 648 1062 55 399 171 1160 359 159 Cuts at: 4531 518 Size: 2042 Fragments arranged by size: 2042 1160 1062 648 399 359 171 159 55 MspA1I CmG'CkG Cuts at: 1606 2062 2596 3471 4148 5296 5541 1606 Size: 456 534 875 677 1148 245 2120 Fragments arranged by size: 2120 1148 875 677 534 456 245 NaeI GCC'GGC Cuts at: 2046 2046 Size: 6055 NarI GG'CG_CC Cuts at: 2048 2048 Size: 6055 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 NdeI CA'TA_TG Cuts at: 387 2850 387 Size: 2463 3592 NgoMIV G'CCGG_C Cuts at: 2044 2044 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NsiI A_TGCA'T Cuts at: 2866 2866 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2233 3413 4958 1132 Size: 97 1004 1180 1545 2229 Fragments arranged by size: 2229 1545 1180 1004 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2078 1108 Size: 970 5085 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PstI C_TGCA'G Cuts at: 830 2697 2835 3407 830 Size: 1867 138 572 3478 Fragments arranged by size: 3478 1867 572 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2596 2596 Size: 6055 SacI G_AGCT'C Cuts at: 2132 2132 Size: 6055 SalI G'TCGA_C Cuts at: 1936 2138 3397 1936 Size: 202 1259 4594 Fragments arranged by size: 4594 1259 202 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2680 2693 2831 3403 4538 5219 5410 Size: 219 1635 13 138 572 1135 681 191 Cuts at: 5410 826 Size: 1471 Fragments arranged by size: 1635 1471 1135 681 572 219 191 138 13 SfoI GGC'GCC Cuts at: 2049 2049 Size: 6055 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2491 2672 4159 5060 5322 820 Size: 197 926 548 181 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 548 262 197 181 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3494 513 Size: 552 939 48 1442 3074 Fragments arranged by size: 3074 1442 939 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2810 4082 4099 4252 4437 4882 325 Size: 1255 1230 1272 17 153 185 445 1498 Fragments arranged by size: 1498 1272 1255 1230 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2764 4301 6013 Size: 274 80 33 51 1443 787 1537 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1537 1443 787 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 6015 6047 2496 Size: 32 3487 32 2504 Fragments arranged by size: 3487 2504 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 2484 4184 2484 Size: 1700 4355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI