################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 104 GGCGCC G[GA]^CG[CT]C AflII 505 CTTAAG C^TTAAG AlwI 675 GGATC GGATC(4/5) BanI 104 GGCGCC G^G[CT][GA]CC BbeI 104 GGCGCC GGCGC^C BbsI 648 GTCTTC GAAGAC(2/6) BfrI 505 CTTAAG C^TTAAG BglI 369 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 648 GTCTTC GAAGAC(2/6) BsaHI 104 GGCGCC G[GA]^CG[CT]C BsaI 520 GAGACC GGTCTC(1/5) BsgI 129 GTGCAG GTGCAG(16/14) BsmFI 688 GTCCC GGGAC(10/14) Bsp1407I 146 TGTACA T^GTACA BsrGI 146 TGTACA T^GTACA BstAPI 345 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 675 GGATCT [GA]^GATC[CT] DdeI 496 CTGAG C^T[ACGT]AG DrdI 529 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 366 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 610 GACGC GACGC(5/10) Hin1I 104 GGCGCC G[GA]^CG[CT]C HindIII 286 AAGCTT A^AGCTT KasI 104 GGCGCC G^GCGCC KspI 135 CCGCGG CCGC^GG MflI 675 GGATCT [GA]^GATC[CT] MseI 506 TTAA T^TAA MslI 162 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 104 GGCGCC GG^CGCC PflMI 524 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 641 AGGACCC [GA]G^G[AT]CC[CT] PstI 262 CTGCAG CTGCA^G PvuII 265 CAGCTG CAG^CTG SacII 135 CCGCGG CCGC^GG SfcI 262 CTGCAG C^T[GA][CT]AG SfoI 104 GGCGCC GGC^GCC SmlI 505 CTTAAG C^T[CT][GA]AG SspBI 146 TGTACA T^GTACA Tru9I 506 TTAA T^TAA Van91I 524 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 668 CCAAGCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 675 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 61 CGCG CG^CG 136 CGCG AvaII 417 GGACC G^G[AT]CC 642 GGACC AviII 278 TGCGCA TGC^GCA 320 TGCGCA BalI 186 TGGCCA TGG^CCA 326 TGGCCA BcnI 117 CCCGG CC^[GC]GG 486 CCCGG BseRI 178 CTCCTC GAGGAG(10/8) 9 GAGGAG BsmAI 498 GAGAC GTCTC(1/5) 520 GAGAC BsmI 387 GAATGC GAATGC(1/-1) 441 GAATGC BsrFI 101 GCCGGC [GA]^CCGG[CT] 227 GCCGGC BstUI 61 CGCG CG^CG 136 CGCG Cfr10I 101 GCCGGC [GA]^CCGG[CT] 227 GCCGGC DpnII 34 GATC ^GATC 676 GATC EaeI 186 TGGCCA [CT]^GGCC[GA] 326 TGGCCA FauI 333 CCCGC CCCGC(4/6) 465 CCCGC FspI 278 TGCGCA TGC^GCA 320 TGCGCA HaeII 104 GGCGCC [GA]GCGC^[CT] 348 GGCGCT HinfI 171 GACTC G^A[ACGT]TC 625 GAGTC HphI 168 GGTGA GGTGA(8/7) 248 TCACC MboI 34 GATC ^GATC 676 GATC MboII 31 GAAGA GAAGA(8/7) 649 TCTTC MluNI 186 TGGCCA TGG^CCA 326 TGGCCA MscI 186 TGGCCA TGG^CCA 326 TGGCCA MvnI 61 CGCG CG^CG 136 CGCG NaeI 101 GCCGGC GCC^GGC 227 GCCGGC NciI 117 CCCGG CC^[GC]GG 486 CCCGG NcoI 356 CCATGG C^CATGG 515 CCATGG NdeII 34 GATC ^GATC 676 GATC NgoMIV 101 GCCGGC G^CCGGC 227 GCCGGC PleI 171 GACTC GAGTC(4/5) 625 GAGTC Sau3AI 34 GATC ^GATC 676 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI