(Circular) (Six-base) MAPSORT of: pFN21AE1335.seq Check: 5418 from: 1 to: 5401 pFN21AE1335 5401 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2445 2445 Size: 5401 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3211 278 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 2498 3464 4848 5116 1632 Size: 848 18 966 1384 268 1917 Fragments arranged by size: 1917 1384 966 848 268 18 AflII C'TTAA_G Cuts at: 820 1017 2510 820 Size: 197 1493 3711 Fragments arranged by size: 3711 1493 197 AflIII A'CryG_T Cuts at: 1203 4300 5277 1203 Size: 3097 977 1327 Fragments arranged by size: 3097 1327 977 AgeI A'CCGG_T Cuts at: 4232 4232 Size: 5401 AhdI GACnn_n'nnGTC Cuts at: 4130 4130 Size: 5401 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5401 AlwNI CAG_nnn'CTG Cuts at: 2028 2266 2356 2683 3144 4716 2028 Size: 238 90 327 461 1572 2713 Fragments arranged by size: 2713 1572 461 327 238 90 ApaI G_GGCC'C Cuts at: 2122 2385 2690 2122 Size: 263 305 4833 Fragments arranged by size: 4833 305 263 ApaLI G'TGCA_C Cuts at: 3458 4614 3458 Size: 1156 4245 ApoI r'AATT_y Cuts at: 1493 2710 2936 3088 5197 1493 Size: 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2726 1870 Size: 73 783 4545 Fragments arranged by size: 4545 783 73 BamHI G'GATC_C Cuts at: 2731 3098 2731 Size: 367 5034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2722 4177 618 Size: 325 217 627 322 613 1455 1842 Fragments arranged by size: 1842 1455 627 613 325 322 217 BanII G_rGCy'C Cuts at: 1391 2122 2149 2371 2385 2690 2720 1391 Size: 731 27 222 14 305 30 4072 Fragments arranged by size: 4072 731 305 222 30 27 14 BbeI G_GCGC'C Cuts at: 2113 2113 Size: 5401 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2646 928 Size: 971 747 3683 Fragments arranged by size: 3683 971 747 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3592 3626 1903 Size: 34 50 34 1571 34 3678 Fragments arranged by size: 3678 1571 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2380 4012 136 Size: 107 122 71 631 605 708 1632 1525 Fragments arranged by size: 1632 1525 708 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2818 2818 Size: 5401 Bme1580I G_kGCm'C Cuts at: 1790 2122 2385 2690 3462 4618 1790 Size: 332 263 305 772 1156 2573 Fragments arranged by size: 2573 1156 772 332 305 263 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5401 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2684 3132 4090 Size: 416 53 83 399 24 1267 448 958 Cuts at: 4090 442 Size: 1753 Fragments arranged by size: 1753 1267 958 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2426 2663 4061 1239 Size: 1055 132 237 1398 2579 Fragments arranged by size: 2579 1398 1055 237 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3526 4391 4689 3526 Size: 865 298 4238 Fragments arranged by size: 4238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2519 4064 882 Size: 617 1020 1545 2219 Fragments arranged by size: 2219 1545 1020 617 BsaAI yAC'GTr Cuts at: 493 1204 3192 493 Size: 711 1988 2702 Fragments arranged by size: 2702 1988 711 BsaBI GATnn'nnATC Cuts at: 5395 5395 Size: 5401 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2110 3208 3590 4225 Size: 53 83 186 936 577 1098 382 635 Cuts at: 4225 275 Size: 1451 Fragments arranged by size: 1451 1098 936 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 4232 4506 4653 5064 3834 Size: 398 274 147 411 4171 Fragments arranged by size: 4171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2117 2147 2602 Size: 30 165 30 657 30 27 30 455 Cuts at: 2602 2632 2636 2666 1178 Size: 30 4 30 3913 Fragments arranged by size: 3913 657 455 165 30 30 30 30 30 30 27 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2174 1186 Size: 843 145 4413 Fragments arranged by size: 4413 843 145 BseYI C'CCAG_C Cuts at: 2101 2662 4604 5100 2101 Size: 561 1942 496 2402 Fragments arranged by size: 2402 1942 561 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2155 1163 Size: 573 419 4409 Fragments arranged by size: 4409 573 419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2851 3612 4640 1540 Size: 275 186 850 761 1028 2301 Fragments arranged by size: 2301 1028 850 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3462 3547 4618 3462 Size: 85 1071 4245 Fragments arranged by size: 4245 1071 85 BsmI GAATG_Cn' Cuts at: 2398 2452 2912 3005 2398 Size: 54 460 93 4794 Fragments arranged by size: 4794 460 93 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2149 2371 2385 2690 2720 3462 Size: 399 332 27 222 14 305 30 742 Cuts at: 3462 3547 4618 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 305 222 85 30 27 14 BspEI T'CCGG_A Cuts at: 5064 5064 Size: 5401 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 2289 2445 2756 844 Size: 1430 15 156 311 3489 Fragments arranged by size: 3489 1430 311 156 15 BsrBI CCG'CTC Cuts at: 1394 1823 3288 1394 Size: 429 1465 3507 Fragments arranged by size: 3507 1465 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 2232 4045 4232 1700 Size: 110 296 126 1813 187 2869 Fragments arranged by size: 2869 1813 296 187 126 110 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 3346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5401 BssSI C'ACGA_G Cuts at: 3461 4473 3461 Size: 1012 4389 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 794 Size: 1562 3839 BstBI TT'CG_AA Cuts at: 4205 4205 Size: 5401 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5401 BstYI r'GATC_y Cuts at: 1972 2680 2731 3098 3483 3500 4952 5038 5050 Size: 708 51 367 385 17 1452 86 12 Cuts at: 5050 5396 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 708 385 367 346 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2140 2361 2520 2697 513 Size: 552 1075 221 159 177 3217 Fragments arranged by size: 3217 1075 552 221 177 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5366 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 4408 809 Size: 507 1224 1868 1802 Fragments arranged by size: 1868 1802 1224 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 2331 2848 Size: 54 1425 50 275 18 361 140 517 Cuts at: 2848 3737 5366 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 517 361 275 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2848 1537 Size: 275 1036 4090 Fragments arranged by size: 4090 1036 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3331 1353 Size: 1978 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3988 4362 4508 387 Size: 1064 138 2399 374 146 1280 Fragments arranged by size: 2399 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2426 2480 2498 2663 3464 4061 Size: 393 662 132 54 18 165 801 597 Cuts at: 4061 4848 5116 1239 Size: 787 268 1524 Fragments arranged by size: 1524 801 787 662 597 393 268 165 132 54 18 EcoNI CCTnn'n_nnAGG Cuts at: 2375 2375 Size: 5401 EcoO109I rG'GnC_Cy Cuts at: 2118 2119 2381 2647 2118 Size: 1 262 266 4872 Fragments arranged by size: 4872 266 262 1 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2503 2503 Size: 5401 FspI TGC'GCA Cuts at: 2285 2327 3907 2285 Size: 42 1580 3779 Fragments arranged by size: 3779 1580 42 FspAI rTGC'GCAy Cuts at: 2327 2327 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 2113 2357 4548 1678 Size: 435 244 2191 2531 Fragments arranged by size: 2531 2191 435 244 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2636 2668 4042 Size: 32 135 32 488 32 544 32 1374 Cuts at: 4042 4074 4116 4148 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1374 544 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 2291 748 Size: 1543 3858 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KasI G'GCGC_C Cuts at: 2109 2109 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 3000 3000 Size: 5401 MluI A'CGCG_T Cuts at: 5277 5277 Size: 5401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 4515 4699 1305 Size: 798 2412 184 2007 Fragments arranged by size: 2412 2007 798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2193 2333 10 Size: 54 1425 343 361 140 3078 Fragments arranged by size: 3078 1425 361 343 140 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 3359 3718 3877 518 Size: 648 1005 1188 359 159 2042 Fragments arranged by size: 2042 1188 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2142 2272 2341 2817 3494 4642 4887 1606 Size: 536 130 69 476 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 476 245 130 69 NaeI GCC'GGC Cuts at: 2108 2234 2108 Size: 126 5275 NarI GG'CG_CC Cuts at: 2110 2110 Size: 5401 NcoI C'CATG_G Cuts at: 513 1065 2361 2520 2697 513 Size: 552 1296 159 177 3217 Fragments arranged by size: 3217 1296 552 177 159 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NgoMIV G'CCGG_C Cuts at: 2106 2232 2106 Size: 126 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2759 4304 1132 Size: 97 1530 1545 2229 Fragments arranged by size: 2229 1545 1530 97 PasI CC'CwG_GG Cuts at: 1348 2114 2377 1348 Size: 766 263 4372 Fragments arranged by size: 4372 766 263 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 2535 1108 Size: 1427 3974 PfoI T'CCnGG_A Cuts at: 2490 2490 Size: 5401 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2647 2647 Size: 5401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PspOMI G'GGCC_C Cuts at: 2118 2381 2686 2118 Size: 263 305 4833 Fragments arranged by size: 4833 305 263 PstI C_TGCA'G Cuts at: 830 2271 2753 830 Size: 1441 482 3478 Fragments arranged by size: 3478 1441 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 PvuII CAG'CTG Cuts at: 2272 2272 Size: 5401 SacII CC_GC'GG Cuts at: 2143 2143 Size: 5401 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2267 2749 3884 4565 4756 826 Size: 219 1222 482 1135 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 482 219 191 SfoI GGC'GCC Cuts at: 2111 2111 Size: 5401 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2728 1872 Size: 856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 3505 4406 4668 820 Size: 197 926 567 995 901 262 1553 Fragments arranged by size: 1553 995 926 901 567 262 197 SnaBI TAC'GTA Cuts at: 493 3192 493 Size: 2699 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2759 1132 Size: 1627 3774 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5401 StyI C'CwwG_G Cuts at: 513 1065 2361 2520 2595 2697 2840 513 Size: 552 1296 159 75 102 143 3074 Fragments arranged by size: 3074 1296 552 159 143 102 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2654 3428 3445 3598 3783 4228 325 Size: 1255 1074 774 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 3647 5359 Size: 274 80 33 51 1443 174 1496 1712 Cuts at: 5359 96 Size: 138 Fragments arranged by size: 1712 1496 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 2602 2634 5361 5393 2117 Size: 32 453 32 2727 32 2125 Fragments arranged by size: 2727 2125 453 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5401 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2680 2680 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5401 XmaI C'CCGG_G Cuts at: 1870 2726 1870 Size: 856 4545 XmnI GAAnn'nnTTC Cuts at: 3530 3530 Size: 5401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3209 276 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI