(Linear) (Six-base) MAPSORT of: KIEE1335.seq Check: 4205 from: 1 to: 696 KIEE1335.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 440 696 Size: 440 256 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 493 696 Size: 475 18 203 Fragments arranged by size: 475 203 18 AflII C'TTAA_G Cuts at: 0 505 696 Size: 505 191 AlwNI CAG_nnn'CTG Cuts at: 0 23 261 351 678 696 Size: 23 238 90 327 18 Fragments arranged by size: 327 238 90 23 18 ApaI G_GGCC'C Cuts at: 0 117 380 685 696 Size: 117 263 305 11 Fragments arranged by size: 305 263 117 11 BanI G'GyrC_C Cuts at: 0 104 696 Size: 104 592 BanII G_rGCy'C Cuts at: 0 117 144 366 380 685 696 Size: 117 27 222 14 305 11 Fragments arranged by size: 305 222 117 27 14 11 BbeI G_GCGC'C Cuts at: 0 108 696 Size: 108 588 BbsI GAAGACnn'nnnn_ Cuts at: 0 641 696 Size: 641 55 BglI GCCn_nnn'nGGC Cuts at: 0 375 696 Size: 375 321 Bme1580I G_kGCm'C Cuts at: 0 117 380 685 696 Size: 117 263 305 11 Fragments arranged by size: 305 263 117 11 BmrI ACTGGGnnnn_n' Cuts at: 0 679 696 Size: 679 17 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 421 658 696 Size: 289 132 237 38 Fragments arranged by size: 289 237 132 38 BsaI GGTCTCn'nnnn_ Cuts at: 0 514 696 Size: 514 182 BsaHI Gr'CG_yC Cuts at: 0 105 696 Size: 105 591 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 55 85 112 142 597 627 631 661 Size: 55 30 27 30 455 30 4 30 Cuts at: 661 696 Size: 35 Fragments arranged by size: 455 55 35 30 30 30 30 27 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 24 169 696 Size: 24 145 527 Fragments arranged by size: 527 145 24 BseYI C'CCAG_C Cuts at: 0 96 657 696 Size: 96 561 39 Fragments arranged by size: 561 96 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 696 Size: 150 546 BsmI GAATG_Cn' Cuts at: 0 393 447 696 Size: 393 54 249 Fragments arranged by size: 393 249 54 Bsp1286I G_dGCh'C Cuts at: 0 117 144 366 380 685 696 Size: 117 27 222 14 305 11 Fragments arranged by size: 305 222 117 27 14 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 269 284 440 696 Size: 269 15 156 256 Fragments arranged by size: 269 256 156 15 BsrFI r'CCGG_y Cuts at: 0 101 227 696 Size: 101 126 469 Fragments arranged by size: 469 126 101 BsrGI T'GTAC_A Cuts at: 0 146 696 Size: 146 550 BstAPI GCAn_nnn'nTGC Cuts at: 0 351 696 Size: 351 345 BstYI r'GATC_y Cuts at: 0 675 696 Size: 675 21 BtgI C'CryG_G Cuts at: 0 135 356 515 696 Size: 135 221 159 181 Fragments arranged by size: 221 181 159 135 DrdI GACnn_nn'nnGTC Cuts at: 0 535 696 Size: 535 161 EaeI y'GGCC_r Cuts at: 0 186 326 696 Size: 186 140 370 Fragments arranged by size: 370 186 140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 421 475 493 658 696 Size: 289 132 54 18 165 38 Fragments arranged by size: 289 165 132 54 38 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 370 696 Size: 370 326 EcoO109I rG'GnC_Cy Cuts at: 0 113 114 376 642 696 Size: 113 1 262 266 54 Fragments arranged by size: 266 262 113 54 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 498 696 Size: 498 198 FspI TGC'GCA Cuts at: 0 280 322 696 Size: 280 42 374 Fragments arranged by size: 374 280 42 FspAI rTGC'GCAy Cuts at: 0 322 696 Size: 322 374 HaeII r_GCGC'y Cuts at: 0 108 352 696 Size: 108 244 344 Fragments arranged by size: 344 244 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 631 663 696 Size: 55 32 544 32 33 Fragments arranged by size: 544 55 33 32 32 HindIII A'AGCT_T Cuts at: 0 286 696 Size: 286 410 KasI G'GCGC_C Cuts at: 0 104 696 Size: 104 592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 98 696 Size: 98 598 MscI TGG'CCA Cuts at: 0 188 328 696 Size: 188 140 368 Fragments arranged by size: 368 188 140 MslI CAynn'nnrTG Cuts at: 0 166 696 Size: 166 530 MspA1I CmG'CkG Cuts at: 0 137 267 336 696 Size: 137 130 69 360 Fragments arranged by size: 360 137 130 69 NaeI GCC'GGC Cuts at: 0 103 229 696 Size: 103 126 467 Fragments arranged by size: 467 126 103 NarI GG'CG_CC Cuts at: 0 105 696 Size: 105 591 NcoI C'CATG_G Cuts at: 0 356 515 696 Size: 356 159 181 Fragments arranged by size: 356 181 159 NgoMIV G'CCGG_C Cuts at: 0 101 227 696 Size: 101 126 469 Fragments arranged by size: 469 126 101 PasI CC'CwG_GG Cuts at: 0 109 372 696 Size: 109 263 324 Fragments arranged by size: 324 263 109 PflMI CCAn_nnn'nTGG Cuts at: 0 530 696 Size: 530 166 PfoI T'CCnGG_A Cuts at: 0 485 696 Size: 485 211 PpuMI rG'GwC_Cy Cuts at: 0 642 696 Size: 642 54 PspOMI G'GGCC_C Cuts at: 0 113 376 681 696 Size: 113 263 305 15 Fragments arranged by size: 305 263 113 15 PstI C_TGCA'G Cuts at: 0 266 696 Size: 266 430 PvuII CAG'CTG Cuts at: 0 267 696 Size: 267 429 SacII CC_GC'GG Cuts at: 0 138 696 Size: 138 558 SfcI C'TryA_G Cuts at: 0 262 696 Size: 262 434 SfoI GGC'GCC Cuts at: 0 106 696 Size: 106 590 SmlI C'TyrA_G Cuts at: 0 505 696 Size: 505 191 StyI C'CwwG_G Cuts at: 0 356 515 590 696 Size: 356 159 75 106 Fragments arranged by size: 356 159 106 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 649 696 Size: 649 47 TatI w'GTAC_w Cuts at: 0 146 696 Size: 146 550 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 112 144 597 629 696 Size: 112 32 453 32 67 Fragments arranged by size: 453 112 67 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 675 696 Size: 675 21 Enzymes that do cut: AarI AcuI AflII AlwNI ApaI BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI BstYI BtgI DrdI EaeI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SfcI SfoI SmlI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV FseI HincII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI