################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AccII 63 CGCG CG^CG AcsI 585 GAATTT [GA]^AATT[CT] ApaI 9 GGGCCC GGGCC^C ApoI 585 GAATTT [GA]^AATT[CT] BciVI 1405 GGATAC GTATCC(6/5) BglII 507 AGATCT A^GATCT Bsp1407I 1550 TGTACA T^GTACA BspHI 737 TCATGA T^CATGA BsrGI 1550 TGTACA T^GTACA BstUI 63 CGCG CG^CG BstYI 507 AGATCT [GA]^GATC[CT] HgaI 64 GCGTC GACGC(5/10) MflI 507 AGATCT [GA]^GATC[CT] MspA1I 151 CAGCTG C[AC]G^C[GT]G MvnI 63 CGCG CG^CG NdeI 1163 CATATG CA^TATG PvuII 151 CAGCTG CAG^CTG RcaI 737 TCATGA T^CATGA SmlI 836 CTCAAG C^T[CT][GA]AG SspBI 1550 TGTACA T^GTACA XbaI 255 TCTAGA T^CTAGA XhoII 507 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Asp700I 311 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1494 GAAGAAGTTC BbsI 1225 GAAGAC GAAGAC(2/6) 1486 GAAGAC BcnI 12 CCCGG CC^[GC]GG 107 CCGGG BfaI 256 CTAG C^TAG 1451 CTAG BpuAI 1225 GAAGAC GAAGAC(2/6) 1486 GAAGAC BsaI 694 GAGACC GGTCTC(1/5) 822 GAGACC BsgI 478 CTGCAC GTGCAG(16/14) 1193 GTGCAG BsrFI 1325 GCCGGT [GA]^CCGG[CT] 1457 ACCGGC CfoI 85 GCGC GCG^C 95 GCGC Cfr10I 1325 GCCGGT [GA]^CCGG[CT] 1457 ACCGGC DraI 1068 TTTAAA TTT^AAA 1480 TTTAAA DraII 1004 GGGACCT [GA]G^G[ACGT]CC[CT] 1311 AGGTCCC EcoO109I 1004 GGGACCT [GA]G^G[ACGT]CC[CT] 1311 AGGTCCC EcoT14I 1000 CCAAGG C^C[AT][AT]GG 1025 CCAAGG HaeII 84 GGCGCT [GA]GCGC^[CT] 94 GGCGCT HhaI 85 GCGC GCG^C 95 GCGC HinP1I 85 GCGC G^CGC 95 GCGC HincII 234 GTCAAC GT[CT]^[GA]AC 903 GTCAAC HindII 234 GTCAAC GT[CT]^[GA]AC 903 GTCAAC MaeI 256 CTAG C^TAG 1451 CTAG MslI 481 CACTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1275 CATTGTTGTG NciI 12 CCCGG CC^[GC]GG 107 CCGGG NspI 878 GCATGT [GA]CATG^[CT] 1319 GCATGT PpuMI 1004 GGGACCT [GA]G^G[AT]CC[CT] 1311 AGGTCCC StyI 1000 CCAAGG C^C[AT][AT]GG 1025 CCAAGG Tsp509I 586 AATT ^AATT 1089 AATT XmnI 311 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1494 GAAGAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I