(Circular) (Six-base) MAPSORT of: pFN21AE1308.seq Check: 3858 from: 1 to: 6277 pFN21AE1308 6277 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 1937 Size: 1683 4594 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2381 2705 3422 3518 3587 4340 5724 Size: 456 293 324 717 96 69 753 1384 Cuts at: 5724 5992 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 753 717 456 324 293 268 96 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2553 1184 Size: 1369 4908 AlwNI CAG_nnn'CTG Cuts at: 2167 2338 3056 4020 5592 2167 Size: 171 718 964 1572 2852 Fragments arranged by size: 2852 1572 964 718 171 ApaI G_GGCC'C Cuts at: 2018 2018 Size: 6277 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2590 3586 3812 3964 6073 1493 Size: 1097 996 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 996 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2025 2231 3453 3596 1391 Size: 627 7 206 1222 143 4072 Fragments arranged by size: 4072 1222 627 206 143 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3237 3498 928 Size: 971 1338 261 3707 Fragments arranged by size: 3707 1338 971 261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4169 5379 3404 Size: 765 1210 4302 Fragments arranged by size: 4302 1210 765 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2512 6272 2512 Size: 3760 2517 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2018 4338 5494 1790 Size: 228 2320 1156 2573 Fragments arranged by size: 2573 2320 1156 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2021 2608 4008 Size: 416 53 83 399 24 604 587 1400 Cuts at: 4008 4966 442 Size: 958 1753 Fragments arranged by size: 1753 1400 958 604 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2722 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2483 2788 3072 4937 1239 Size: 974 270 305 284 1865 2579 Fragments arranged by size: 2579 1865 974 305 284 270 Bpu10I CC'TnA_GC Cuts at: 3387 3387 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2826 4402 5267 5565 2826 Size: 1576 865 298 3538 Fragments arranged by size: 3538 1576 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2693 2821 4940 882 Size: 617 1194 128 2119 2219 Fragments arranged by size: 2219 2119 1194 617 128 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4710 5108 5382 5529 5940 4710 Size: 398 274 147 411 5047 Fragments arranged by size: 5047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 2380 2708 2711 2724 2861 2887 1186 Size: 1035 159 328 3 13 137 26 4576 Fragments arranged by size: 4576 1035 328 159 137 26 13 3 BseYI C'CCAG_C Cuts at: 2542 3056 5480 5976 2542 Size: 514 2424 496 2843 Fragments arranged by size: 2843 2424 514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2468 3219 1163 Size: 573 732 751 4221 Fragments arranged by size: 4221 751 732 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4338 4423 5494 4338 Size: 85 1071 5121 Fragments arranged by size: 5121 1071 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2025 2231 3453 3596 4338 4423 Size: 399 228 7 206 1222 143 742 85 Cuts at: 4423 5494 1391 Size: 1071 2174 Fragments arranged by size: 2174 1222 1071 742 399 228 206 143 85 7 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 2742 4166 5896 2742 Size: 1424 1730 3123 Fragments arranged by size: 3123 1730 1424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3632 844 Size: 2788 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3330 3462 4921 5108 1700 Size: 110 1520 132 1459 187 2869 Fragments arranged by size: 2869 1520 1459 187 132 110 BsrGI T'GTAC_A Cuts at: 96 3555 96 Size: 3459 2818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 2472 2707 3476 4337 5349 2472 Size: 235 769 861 1012 3400 Fragments arranged by size: 3400 1012 861 769 235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2512 3607 3974 4359 4376 5828 5914 5926 Size: 540 1095 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1095 540 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4786 Fragments arranged by size: 4786 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2584 3207 3292 3793 4587 4607 2179 Size: 405 623 85 501 794 20 3849 Fragments arranged by size: 3849 794 623 501 405 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3075 3487 3581 3075 Size: 412 94 5771 Fragments arranged by size: 5771 412 94 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3724 4613 6242 Size: 54 1425 50 275 18 1894 889 1629 Cuts at: 6242 8 Size: 43 Fragments arranged by size: 1894 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2357 2522 2819 2873 2943 3216 3533 Size: 698 306 165 297 54 70 273 317 Cuts at: 3533 4207 1353 Size: 674 3423 Fragments arranged by size: 3423 698 674 317 306 297 273 165 70 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2213 2381 2483 2705 2788 3072 Size: 393 456 125 168 102 222 83 284 Cuts at: 3072 3422 3518 3587 4340 4937 5724 5992 1239 Size: 350 96 69 753 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 456 393 350 284 268 222 168 125 102 96 83 69 EcoO109I rG'GnC_Cy Cuts at: 3010 3317 3010 Size: 307 5970 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2312 3439 2312 Size: 1127 5150 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 2093 2103 5424 1678 Size: 415 10 3321 2531 Fragments arranged by size: 3321 2531 415 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2719 2751 2926 2958 3174 Size: 32 135 32 1147 32 175 32 216 Cuts at: 3174 3206 3544 3576 4918 4950 4992 5024 1373 Size: 32 338 32 1342 32 42 32 2626 Fragments arranged by size: 2626 1342 1147 338 216 175 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2241 2910 3621 3867 669 Size: 988 281 303 669 711 246 3079 Fragments arranged by size: 3079 988 711 669 303 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5391 5575 1305 Size: 4086 184 2007 Fragments arranged by size: 4086 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4455 Fragments arranged by size: 4455 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2490 3284 4235 4594 4753 518 Size: 648 1324 794 951 359 159 2042 Fragments arranged by size: 2042 1324 951 794 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 3693 4370 5518 5763 1606 Size: 552 1535 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 677 552 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4786 Fragments arranged by size: 4786 939 552 NdeI CA'TA_TG Cuts at: 387 3169 387 Size: 2782 3495 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2887 3328 3635 5180 1132 Size: 97 1658 441 307 1545 2229 Fragments arranged by size: 2229 1658 1545 441 307 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PfoI T'CCnGG_A Cuts at: 2499 2499 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3010 3317 3010 Size: 307 5970 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 2014 2014 Size: 6277 PstI C_TGCA'G Cuts at: 830 2057 2163 2220 3629 830 Size: 1227 106 57 1409 3478 Fragments arranged by size: 3478 1409 1227 106 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2158 2158 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SapI GCTCTTCn'nnn_ Cuts at: 2051 2873 3533 2051 Size: 822 660 4795 Fragments arranged by size: 4795 822 660 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2053 2159 2216 3625 4760 5441 5632 Size: 219 1008 106 57 1409 1135 681 191 Cuts at: 5632 826 Size: 1471 Fragments arranged by size: 1471 1409 1135 1008 681 219 191 106 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2841 4381 5282 5544 820 Size: 197 926 898 1540 901 262 1553 Fragments arranged by size: 1553 1540 926 901 898 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2004 3005 3030 3716 513 Size: 552 939 1001 25 686 3074 Fragments arranged by size: 3074 1001 939 686 552 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2802 4304 4321 4474 4659 5104 325 Size: 1255 1222 1502 17 153 185 445 1498 Fragments arranged by size: 1502 1498 1255 1222 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2864 3555 4523 Size: 274 80 33 51 1443 887 691 968 Cuts at: 4523 6235 96 Size: 1712 138 Fragments arranged by size: 1712 1443 968 887 691 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 2260 3613 2260 Size: 1353 4924 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 2320 3503 4406 2320 Size: 1183 903 4191 Fragments arranged by size: 4191 1183 903 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI