################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AviII BalI BbeI BbrPI BbsI BcgI BciVI BfrI BglII BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CpoI DraI DraIII DrdI Eco81I EcoNI EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 392 GACGTC GACGT^C AcyI 392 GACGTC G[GA]^CG[CT]C AfaI 1494 GTAC GT^AC AhdI 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1061 AGCGCT AGC^GCT ApaI 848 GGGCCC GGGCC^C Asp700I 12 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 514 GAGCAC G[AT]GC[AT]^C AspI 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1264 CCTAGG C^CTAGG BamHI 795 GGATCC G^GATCC BanI 1582 GGCACC G^G[CT][GA]CC BclI 1127 TGATCA T^GATCA BlnI 1264 CCTAGG C^CTAGG BlpI 1677 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1677 GCTGAGC GC^T[ACGT]AGC BsaHI 392 GACGTC G[GA]^CG[CT]C BsgI 1357 GTGCAG GTGCAG(16/14) BsiEI 1533 CGGCCG CG[GA][CT]^CG BsiHKAI 514 GAGCAC G[AT]GC[AT]^C Bsp1407I 1493 TGTACA T^GTACA BspDI 236 ATCGAT AT^CGAT BsrDI 1033 GCAATG GCAATG(2/0) BsrFI 318 GCCGGT [GA]^CCGG[CT] BsrGI 1493 TGTACA T^GTACA BssSI 218 CACGAG CACGAG(-5/-1) BstAPI 1359 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1080 GGTGACC G^GT[ACGT]ACC BstPI 1080 GGTGACC G^GT[ACGT]ACC CelII 1677 GCTGAGC GC^T[ACGT]AGC Cfr10I 318 GCCGGT [GA]^CCGG[CT] ClaI 236 ATCGAT AT^CGAT DsaI 1607 CCACGG C^C[GA][CT]GG EagI 1533 CGGCCG C^GGCCG Eam1105I 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1533 CGGCCG C^GGCCG Eco47III 1061 AGCGCT AGC^GCT Eco52I 1533 CGGCCG C^GGCCG EcoO65I 1080 GGTGACC G^GT[ACGT]ACC EcoRI 1349 GAATTC G^AATTC EcoRV 586 GATATC GAT^ATC FbaI 1127 TGATCA T^GATCA HaeII 1061 AGCGCT [GA]GCGC^[CT] Hin1I 392 GACGTC G[GA]^CG[CT]C MseI 188 TTAA T^TAA PflFI 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 1494 GTAC GT^AC SapI 1672 GAAGAGC GCTCTTC(1/4) SfaNI 1717 GATGC GCATC(5/9) SmlI 909 CTTGAG C^T[CT][GA]AG SspBI 1493 TGTACA T^GTACA StuI 1239 AGGCCT AGG^CCT Tru9I 188 TTAA T^TAA Tth111I 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 12 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 606 GAATTT [GA]^AATT[CT] 1349 GAATTC ApoI 606 GAATTT [GA]^AATT[CT] 1349 GAATTC BanII 848 GGGCCC G[GA]GC[CT]^C 1315 GAGCCC BfaI 800 CTAG C^TAG 1265 CTAG BglI 1471 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1775 GCCTGGCTGGC BsaI 1603 GAGACC GGTCTC(1/5) 1747 GAGACC BsrBI 61 GAGCGG CCGCTC(-3/-3) 124 GAGCGG BstXI 887 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1607 CCACGGAGCTGG EaeI 1533 CGGCCG [CT]^GGCC[GA] 1782 TGGCCG MaeI 800 CTAG C^TAG 1265 CTAG MslI 888 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1323 CACTGCTGTG NlaIII 478 CATG CATG^ 530 CATG PpuMI 776 GGGACCC [GA]G^G[AT]CC[CT] 1394 AGGACCC PvuII 538 CAGCTG CAG^CTG 915 CAGCTG SfcI 313 CTACAG C^T[GA][CT]AG 1566 CTACAG SmaI 299 CCCGGG CCC^GGG 1173 CCCGGG TaqI 237 TCGA T^CGA 390 TCGA TfiI 474 GATTC G^A[AT]TC 1027 GATTC Tsp509I 607 AATT ^AATT 1350 AATT TthHB8I 237 TCGA T^CGA 390 TCGA XmaCI 299 CCCGGG C^CCGGG 1173 CCCGGG XmaI 299 CCCGGG C^CCGGG 1173 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaI AvaII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI XcmI XhoII