Restriction map for pFN21AE5716
 (Linear) (Six-base) MAPSORT of: KIEE5716.seq  Check: 1862  from: 1  to: 1821



KIEE5716.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1382    1636    1821 
   Size:       1382     254     185

  Fragments arranged by size: 

                1382     254     185
 
AatII G_ACGT'C

Cuts at:      0     396    1821 
   Size:        396    1425
 
AfeI AGC'GCT

Cuts at:      0    1063    1821 
   Size:       1063     758
 
AhdI GACnn_n'nnGTC

Cuts at:      0     159    1821 
   Size:        159    1662
 
AleI CACnn'nnGTG

Cuts at:      0    1327    1821 
   Size:       1327     494
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469    1821 
   Size:        437      32    1352

  Fragments arranged by size: 

                1352     437      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     870    1281    1365    1390    1688    1821 
   Size:        210     660     411      84      25     298     133

  Fragments arranged by size: 

                 660     411     298     210     133      84      25
 
ApaI G_GGCC'C

Cuts at:      0     852    1821 
   Size:        852     969
 
ApoI r'AATT_y

Cuts at:      0     606    1349    1821 
   Size:        606     743     472

  Fragments arranged by size: 

                 743     606     472
 
AvaI C'yCGr_G

Cuts at:      0     299     773     845     935    1173    1821 
   Size:        299     474      72      90     238     648

  Fragments arranged by size: 

                 648     474     299     238      90      72
 
AvrII C'CTAG_G

Cuts at:      0    1264    1821 
   Size:       1264     557
 
BamHI G'GATC_C

Cuts at:      0     795    1821 
   Size:        795    1026
 
BanI G'GyrC_C

Cuts at:      0    1582    1821 
   Size:       1582     239
 
BanII G_rGCy'C

Cuts at:      0     852    1319    1821 
   Size:        852     467     502

  Fragments arranged by size: 

                 852     502     467
 
BbvCI CC'TCA_GC

Cuts at:      0    1391    1401    1540    1821 
   Size:       1391      10     139     281

  Fragments arranged by size: 

                1391     281     139      10
 
BclI T'GATC_A

Cuts at:      0    1127    1821 
   Size:       1127     694
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1477    1781    1821 
   Size:       1477     304      40

  Fragments arranged by size: 

                1477     304      40
 
BlpI GC'TnA_GC

Cuts at:      0    1678    1821 
   Size:       1678     143
 
Bme1580I G_kGCm'C

Cuts at:      0     852    1821 
   Size:        852     969
 
BmgBI CAC'GTC

Cuts at:      0     750    1821 
   Size:        750    1071
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1416    1821 
   Size:       1416     405
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     544     659    1149    1748    1821 
   Size:         78     466     115     490     599      73

  Fragments arranged by size: 

                 599     490     466     115      78      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    1391    1401    1540    1821 
   Size:       1391      10     139     281

  Fragments arranged by size: 

                1391     281     139      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     930    1821 
   Size:        930     891
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1597    1741    1821 
   Size:       1597     144      80

  Fragments arranged by size: 

                1597     144      80
 
BsaHI Gr'CG_yC

Cuts at:      0     393    1821 
   Size:        393    1428
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1327    1357    1746    1776    1821 
   Size:       1327      30     389      30      45

  Fragments arranged by size: 

                1327     389      45      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286     297     480     991     994    1003    1669    1672 
   Size:        286      11     183     511       3       9     666       3

Cuts at:   1672    1675    1708    1711    1720    1741    1759    1821 
   Size:          3      33       3       9      21      18      62

  Fragments arranged by size: 

                 666     511     286     183      62      33      21      18
                  11       9       9       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     210     740     946     999    1803    1821 
   Size:        210     530     206      53     804      18

  Fragments arranged by size: 

                 804     530     210     206      53      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1378    1821 
   Size:       1378     443
 
BsiEI CG_ry'CG

Cuts at:      0    1536    1821 
   Size:       1536     285
 
BsiHKAI G_wGCw'C

Cuts at:      0     518    1821 
   Size:        518    1303
 
Bsp1286I G_dGCh'C

Cuts at:      0     518     852    1319    1821 
   Size:        518     334     467     502

  Fragments arranged by size: 

                 518     502     467     334
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1266    1382    1636    1821 
   Size:       1266     116     254     185

  Fragments arranged by size: 

                1266     254     185     116
 
BsrBI CCG'CTC

Cuts at:      0      63     126    1821 
   Size:         63      63    1695

  Fragments arranged by size: 

                1695      63      63
 
BsrDI GCAATG_nn'

Cuts at:      0    1040    1821 
   Size:       1040     781
 
BsrFI r'CCGG_y

Cuts at:      0     318    1821 
   Size:        318    1503
 
BsrGI T'GTAC_A

Cuts at:      0    1493    1821 
   Size:       1493     328
 
BssSI C'ACGA_G

Cuts at:      0     218    1821 
   Size:        218    1603
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1365    1821 
   Size:       1365     456
 
BstEII G'GTnAC_C

Cuts at:      0    1080    1821 
   Size:       1080     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     894    1614    1821 
   Size:        894     720     207

  Fragments arranged by size: 

                 894     720     207
 
BstYI r'GATC_y

Cuts at:      0     681     795    1289    1307    1821 
   Size:        681     114     494      18     514

  Fragments arranged by size: 

                 681     514     494     114      18
 
BtgI C'CryG_G

Cuts at:      0    1607    1821 
   Size:       1607     214
 
BtsI GCAGTG_nn'

Cuts at:      0    1322    1532    1821 
   Size:       1322     210     289

  Fragments arranged by size: 

                1322     289     210
 
ClaI AT'CG_AT

Cuts at:      0     237    1821 
   Size:        237    1584
 
EaeI y'GGCC_r

Cuts at:      0    1533    1782    1821 
   Size:       1533     249      39

  Fragments arranged by size: 

                1533     249      39
 
EagI C'GGCC_G

Cuts at:      0    1533    1821 
   Size:       1533     288
 
EarI CTCTTCn'nnn_

Cuts at:      0      61     811     980    1262    1661    1667    1821 
   Size:         61     750     169     282     399       6     154

  Fragments arranged by size: 

                 750     399     282     169     154      61       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1161    1821 
   Size:       1161     660
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     544     659    1149    1748    1821 
   Size:         78     466     115     490     599      73

  Fragments arranged by size: 

                 599     490     466     115      78      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     777     849    1362    1395    1821 
   Size:        777      72     513      33     426

  Fragments arranged by size: 

                 777     513     426      72      33
 
EcoRI G'AATT_C

Cuts at:      0    1349    1821 
   Size:       1349     472
 
EcoRV GAT'ATC

Cuts at:      0     588    1821 
   Size:        588    1233
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     481    1821 
   Size:        481    1340
 
HaeII r_GCGC'y

Cuts at:      0    1065    1821 
   Size:       1065     756
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177    1821 
   Size:        145      32    1644

  Fragments arranged by size: 

                1644     145      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     317     615     802    1821 
   Size:        125     192     298     187    1019

  Fragments arranged by size: 

                1019     298     192     187     125
 
MslI CAynn'nnrTG

Cuts at:      0     892    1327    1821 
   Size:        892     435     494

  Fragments arranged by size: 

                 892     494     435
 
MspA1I CmG'CkG

Cuts at:      0     113     540     917    1405    1821 
   Size:        113     427     377     488     416

  Fragments arranged by size: 

                 488     427     416     377     113
 
PasI CC'CwG_GG

Cuts at:      0     852    1821 
   Size:        852     969
 
PfoI T'CCnGG_A

Cuts at:      0     350    1310    1821 
   Size:        350     960     511

  Fragments arranged by size: 

                 960     511     350
 
PpuMI rG'GwC_Cy

Cuts at:      0     777    1395    1821 
   Size:        777     618     426

  Fragments arranged by size: 

                 777     618     426
 
PspOMI G'GGCC_C

Cuts at:      0     848    1821 
   Size:        848     973
 
PvuII CAG'CTG

Cuts at:      0     540     917    1821 
   Size:        540     377     904

  Fragments arranged by size: 

                 904     540     377
 
SanDI GG'GwC_CC

Cuts at:      0     777    1821 
   Size:        777    1044
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1667    1821 
   Size:       1667     154
 
SfcI C'TryA_G

Cuts at:      0     313    1566    1821 
   Size:        313    1253     255

  Fragments arranged by size: 

                1253     313     255
 
SmaI CCC'GGG

Cuts at:      0     301    1175    1821 
   Size:        301     874     646

  Fragments arranged by size: 

                 874     646     301
 
SmlI C'TyrA_G

Cuts at:      0     909    1821 
   Size:        909     912
 
StuI AGG'CCT

Cuts at:      0    1241    1821 
   Size:       1241     580
 
StyI C'CwwG_G

Cuts at:      0     400     419    1264    1472    1821 
   Size:        400      19     845     208     349

  Fragments arranged by size: 

                 845     400     349     208      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     157    1397    1795    1821 
   Size:        157    1240     398      26

  Fragments arranged by size: 

                1240     398     157      26
 
TatI w'GTAC_w

Cuts at:      0    1493    1821 
   Size:       1493     328
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     753     785    1821 
   Size:        753      32    1036

  Fragments arranged by size: 

                1036     753      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     568    1821 
   Size:        568    1253
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     463    1092    1614    1821 
   Size:        463     629     522     207

  Fragments arranged by size: 

                 629     522     463     207
 
XmaI C'CCGG_G

Cuts at:      0     299    1173    1821 
   Size:        299     874     648

  Fragments arranged by size: 

                 874     648     299
 
XmnI GAAnn'nnTTC

Cuts at:      0      16    1821 
   Size:         16    1805
 
ZraI GAC'GTC

Cuts at:      0     394    1821 
   Size:        394    1427
 

 Enzymes that do cut:

     AarI    AatII     AfeI     AhdI     AleI     AloI    AlwNI     ApaI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII    BbvCI     BclI
     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I     MmeI     MslI   MspA1I     PasI     PfoI    PpuMI   PspOMI
    PvuII    SanDI     SapI     SfcI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AlfI
    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI     BbsI     BcgI
    BciVI    BfrBI    BglII     BmrI     BmtI    BsaAI    BsaBI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII    BstBI  BstZ17I
   Bsu36I    BtgZI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI
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