(Linear) (Six-base) MAPSORT of: KIEE5716.seq Check: 1862 from: 1 to: 1821 KIEE5716.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1382 1636 1821 Size: 1382 254 185 Fragments arranged by size: 1382 254 185 AatII G_ACGT'C Cuts at: 0 396 1821 Size: 396 1425 AfeI AGC'GCT Cuts at: 0 1063 1821 Size: 1063 758 AhdI GACnn_n'nnGTC Cuts at: 0 159 1821 Size: 159 1662 AleI CACnn'nnGTG Cuts at: 0 1327 1821 Size: 1327 494 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 437 469 1821 Size: 437 32 1352 Fragments arranged by size: 1352 437 32 AlwNI CAG_nnn'CTG Cuts at: 0 210 870 1281 1365 1390 1688 1821 Size: 210 660 411 84 25 298 133 Fragments arranged by size: 660 411 298 210 133 84 25 ApaI G_GGCC'C Cuts at: 0 852 1821 Size: 852 969 ApoI r'AATT_y Cuts at: 0 606 1349 1821 Size: 606 743 472 Fragments arranged by size: 743 606 472 AvaI C'yCGr_G Cuts at: 0 299 773 845 935 1173 1821 Size: 299 474 72 90 238 648 Fragments arranged by size: 648 474 299 238 90 72 AvrII C'CTAG_G Cuts at: 0 1264 1821 Size: 1264 557 BamHI G'GATC_C Cuts at: 0 795 1821 Size: 795 1026 BanI G'GyrC_C Cuts at: 0 1582 1821 Size: 1582 239 BanII G_rGCy'C Cuts at: 0 852 1319 1821 Size: 852 467 502 Fragments arranged by size: 852 502 467 BbvCI CC'TCA_GC Cuts at: 0 1391 1401 1540 1821 Size: 1391 10 139 281 Fragments arranged by size: 1391 281 139 10 BclI T'GATC_A Cuts at: 0 1127 1821 Size: 1127 694 BglI GCCn_nnn'nGGC Cuts at: 0 1477 1781 1821 Size: 1477 304 40 Fragments arranged by size: 1477 304 40 BlpI GC'TnA_GC Cuts at: 0 1678 1821 Size: 1678 143 Bme1580I G_kGCm'C Cuts at: 0 852 1821 Size: 852 969 BmgBI CAC'GTC Cuts at: 0 750 1821 Size: 750 1071 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1416 1821 Size: 1416 405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 544 659 1149 1748 1821 Size: 78 466 115 490 599 73 Fragments arranged by size: 599 490 466 115 78 73 Bpu10I CC'TnA_GC Cuts at: 0 1391 1401 1540 1821 Size: 1391 10 139 281 Fragments arranged by size: 1391 281 139 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 930 1821 Size: 930 891 BsaI GGTCTCn'nnnn_ Cuts at: 0 1597 1741 1821 Size: 1597 144 80 Fragments arranged by size: 1597 144 80 BsaHI Gr'CG_yC Cuts at: 0 393 1821 Size: 393 1428 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1327 1357 1746 1776 1821 Size: 1327 30 389 30 45 Fragments arranged by size: 1327 389 45 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 297 480 991 994 1003 1669 1672 Size: 286 11 183 511 3 9 666 3 Cuts at: 1672 1675 1708 1711 1720 1741 1759 1821 Size: 3 33 3 9 21 18 62 Fragments arranged by size: 666 511 286 183 62 33 21 18 11 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 210 740 946 999 1803 1821 Size: 210 530 206 53 804 18 Fragments arranged by size: 804 530 210 206 53 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1378 1821 Size: 1378 443 BsiEI CG_ry'CG Cuts at: 0 1536 1821 Size: 1536 285 BsiHKAI G_wGCw'C Cuts at: 0 518 1821 Size: 518 1303 Bsp1286I G_dGCh'C Cuts at: 0 518 852 1319 1821 Size: 518 334 467 502 Fragments arranged by size: 518 502 467 334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1266 1382 1636 1821 Size: 1266 116 254 185 Fragments arranged by size: 1266 254 185 116 BsrBI CCG'CTC Cuts at: 0 63 126 1821 Size: 63 63 1695 Fragments arranged by size: 1695 63 63 BsrDI GCAATG_nn' Cuts at: 0 1040 1821 Size: 1040 781 BsrFI r'CCGG_y Cuts at: 0 318 1821 Size: 318 1503 BsrGI T'GTAC_A Cuts at: 0 1493 1821 Size: 1493 328 BssSI C'ACGA_G Cuts at: 0 218 1821 Size: 218 1603 BstAPI GCAn_nnn'nTGC Cuts at: 0 1365 1821 Size: 1365 456 BstEII G'GTnAC_C Cuts at: 0 1080 1821 Size: 1080 741 BstXI CCAn_nnnn'nTGG Cuts at: 0 894 1614 1821 Size: 894 720 207 Fragments arranged by size: 894 720 207 BstYI r'GATC_y Cuts at: 0 681 795 1289 1307 1821 Size: 681 114 494 18 514 Fragments arranged by size: 681 514 494 114 18 BtgI C'CryG_G Cuts at: 0 1607 1821 Size: 1607 214 BtsI GCAGTG_nn' Cuts at: 0 1322 1532 1821 Size: 1322 210 289 Fragments arranged by size: 1322 289 210 ClaI AT'CG_AT Cuts at: 0 237 1821 Size: 237 1584 EaeI y'GGCC_r Cuts at: 0 1533 1782 1821 Size: 1533 249 39 Fragments arranged by size: 1533 249 39 EagI C'GGCC_G Cuts at: 0 1533 1821 Size: 1533 288 EarI CTCTTCn'nnn_ Cuts at: 0 61 811 980 1262 1661 1667 1821 Size: 61 750 169 282 399 6 154 Fragments arranged by size: 750 399 282 169 154 61 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1161 1821 Size: 1161 660 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 544 659 1149 1748 1821 Size: 78 466 115 490 599 73 Fragments arranged by size: 599 490 466 115 78 73 EcoO109I rG'GnC_Cy Cuts at: 0 777 849 1362 1395 1821 Size: 777 72 513 33 426 Fragments arranged by size: 777 513 426 72 33 EcoRI G'AATT_C Cuts at: 0 1349 1821 Size: 1349 472 EcoRV GAT'ATC Cuts at: 0 588 1821 Size: 588 1233 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 481 1821 Size: 481 1340 HaeII r_GCGC'y Cuts at: 0 1065 1821 Size: 1065 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 145 177 1821 Size: 145 32 1644 Fragments arranged by size: 1644 145 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 125 317 615 802 1821 Size: 125 192 298 187 1019 Fragments arranged by size: 1019 298 192 187 125 MslI CAynn'nnrTG Cuts at: 0 892 1327 1821 Size: 892 435 494 Fragments arranged by size: 892 494 435 MspA1I CmG'CkG Cuts at: 0 113 540 917 1405 1821 Size: 113 427 377 488 416 Fragments arranged by size: 488 427 416 377 113 PasI CC'CwG_GG Cuts at: 0 852 1821 Size: 852 969 PfoI T'CCnGG_A Cuts at: 0 350 1310 1821 Size: 350 960 511 Fragments arranged by size: 960 511 350 PpuMI rG'GwC_Cy Cuts at: 0 777 1395 1821 Size: 777 618 426 Fragments arranged by size: 777 618 426 PspOMI G'GGCC_C Cuts at: 0 848 1821 Size: 848 973 PvuII CAG'CTG Cuts at: 0 540 917 1821 Size: 540 377 904 Fragments arranged by size: 904 540 377 SanDI GG'GwC_CC Cuts at: 0 777 1821 Size: 777 1044 SapI GCTCTTCn'nnn_ Cuts at: 0 1667 1821 Size: 1667 154 SfcI C'TryA_G Cuts at: 0 313 1566 1821 Size: 313 1253 255 Fragments arranged by size: 1253 313 255 SmaI CCC'GGG Cuts at: 0 301 1175 1821 Size: 301 874 646 Fragments arranged by size: 874 646 301 SmlI C'TyrA_G Cuts at: 0 909 1821 Size: 909 912 StuI AGG'CCT Cuts at: 0 1241 1821 Size: 1241 580 StyI C'CwwG_G Cuts at: 0 400 419 1264 1472 1821 Size: 400 19 845 208 349 Fragments arranged by size: 845 400 349 208 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 157 1397 1795 1821 Size: 157 1240 398 26 Fragments arranged by size: 1240 398 157 26 TatI w'GTAC_w Cuts at: 0 1493 1821 Size: 1493 328 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 753 785 1821 Size: 753 32 1036 Fragments arranged by size: 1036 753 32 Tth111I GACn'n_nGTC Cuts at: 0 568 1821 Size: 568 1253 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 463 1092 1614 1821 Size: 463 629 522 207 Fragments arranged by size: 629 522 463 207 XmaI C'CCGG_G Cuts at: 0 299 1173 1821 Size: 299 874 648 Fragments arranged by size: 874 648 299 XmnI GAAnn'nnTTC Cuts at: 0 16 1821 Size: 16 1805 ZraI GAC'GTC Cuts at: 0 394 1821 Size: 394 1427 Enzymes that do cut: AarI AatII AfeI AhdI AleI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbvCI BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I MmeI MslI MspA1I PasI PfoI PpuMI PspOMI PvuII SanDI SapI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI ApaLI AscI AseI AsiSI BaeI BbeI BbsI BcgI BciVI BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EcoICRI EcoNI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI