Restriction map for
pFN21AB6754
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BseAI
BsgI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HphI ItaI KasI KpnI KspI
MaeI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI TseI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 154 GTAC GT^AC
AluI 260 AGCT AG^CT
AlwNI 379 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 381 GGACC G^G[AT]CC
BamHI 342 GGATCC G^GATCC
BbsI 397 GTCTTC GAAGAC(2/6)
BclI 134 TGATCA T^GATCA
BmyI 34 GGGCAC G[AGT]GC[ACT]^C
BpmI 64 CTGGAG CTGGAG(16/14)
BpuAI 397 GTCTTC GAAGAC(2/6)
BsaAI 28 TACGTG [CT]AC^GT[GA]
BsaBI 325 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 338 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 81 ACCGGA [AT]^CCGG[AT]
BseRI 233 CTCCTC GAGGAG(10/8)
BsiEI 79 CGACCG CG[GA][CT]^CG
Bsp1286I 34 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 153 TGTACA T^GTACA
BspHI 131 TCATGA T^CATGA
BsrFI 251 GCCGGC [GA]^CCGG[CT]
BsrGI 153 TGTACA T^GTACA
BssSI 55 CACGAG CACGAG(-5/-1)
BstF5I 216 GGATG GGATG(2/0)
BstYI 342 GGATCC [GA]^GATC[CT]
Cac8I 251 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 251 GCCGGC [GA]^CCGG[CT]
DraII 380 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 51 CGGCCA [CT]^GGCC[GA]
EarI 180 GAAGAG CTCTTC(1/4)
EcoO109I 380 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 338 CCAAGG C^C[AT][AT]GG
EcoT22I 221 ATGCAT ATGCA^T
FbaI 134 TGATCA T^GATCA
FokI 216 GGATG GGATG(9/13)
Ksp632I 180 GAAGAG CTCTTC(1/4)
MaeII 29 ACGT A^CGT
MamI 325 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 342 GGATCC [GA]^GATC[CT]
MwoI 44 GCAAGTTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 251 GCCGGC GCC^GGC
NgoMIV 251 GCCGGC G^CCGGC
NlaIII 132 CATG CATG^
NsiI 221 ATGCAT ATGCA^T
PpuMI 380 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 131 TCATGA T^CATGA
RsaI 154 GTAC GT^AC
SfaNI 220 GATGC GCATC(5/9)
SspBI 153 TGTACA T^GTACA
StyI 338 CCAAGG C^C[AT][AT]GG
TaqI 78 TCGA T^CGA
Tsp45I 8 GTGAC ^GT[GC]AC
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 78 TCGA T^CGA
XhoII 342 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 343 GATCC GGATC(4/5)
342 GGATC
BssKI 63 CCTGG ^CC[ACGT]GG
378 CCAGG
BstNI 63 CCTGG CC^[AT]GG
378 CCAGG
Cfr13I 249 GGGCC G^G[ACGT]CC
381 GGACC
DpnII 135 GATC ^GATC
343 GATC
EcoRII 63 CCTGG ^CC[AT]GG
378 CCAGG
HapII 82 CCGG C^CGG
252 CCGG
HpaII 82 CCGG C^CGG
252 CCGG
MaeIII 8 GTGAC ^GT[ACGT]AC
23 GTTAC
MboI 135 GATC ^GATC
343 GATC
MboII 180 GAAGA GAAGA(8/7)
398 TCTTC
MseI 94 TTAA T^TAA
428 TTAA
MspI 82 CCGG C^CGG
252 CCGG
MvaI 63 CCTGG CC^[AT]GG
378 CCAGG
NdeII 135 GATC ^GATC
343 GATC
NlaIV 313 GGTTCC GG[ACGT]^[ACGT]CC
342 GGATCC
Sau3AI 135 GATC ^GATC
343 GATC
Sau96I 249 GGGCC G^G[ACGT]CC
381 GGACC
ScrFI 63 CCTGG CC^[ACGT]GG
378 CCAGG
Tru9I 94 TTAA T^TAA
428 TTAA
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
HaeIII MnlI Tsp509I