################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsgI BsiWI BsmI BsoBI BspDI BspHI BspLU11I BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MamI MfeI MflI MluI MseI MslI MunI NciI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfaNI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 494 TCCGGA T^CCGGA AflIII 272 ACGTGT A^C[GA][CT]GT AlwI 471 GGATC GGATC(4/5) AlwNI 502 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 139 GGGCCC GGGCC^C AscI 177 GGCGCGCC GG^CGCGCC AspHI 46 GAGCTC G[AT]GC[AT]^C BalI 128 TGGCCA TGG^CCA BbsI 576 GAAGAC GAAGAC(2/6) BfaI 13 CTAG C^TAG BpmI 43 CTGGAG CTGGAG(16/14) BpuAI 576 GAAGAC GAAGAC(2/6) BsaWI 494 TCCGGA [AT]^CCGG[AT] BseAI 494 TCCGGA T^CCGGA BsiHKAI 46 GAGCTC G[AT]GC[AT]^C BsmAI 262 GAGAC GTCTC(1/5) BsmBI 262 GAGACG CGTCTC(1/5) Bsp1407I 440 TGTACA T^GTACA BspEI 494 TCCGGA T^CCGGA BspMI 501 GCAGGT ACCTGC(4/8) BsrBI 315 GAGCGG CCGCTC(-3/-3) BsrGI 440 TGTACA T^GTACA BssHII 178 GCGCGC G^CGCGC BssKI 507 CCTGG ^CC[ACGT]GG BstNI 507 CCTGG CC^[AT]GG BstXI 551 CCACCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 7 CGGCCG C^GGCCG EclXI 7 CGGCCG C^GGCCG Eco52I 7 CGGCCG C^GGCCG EcoNI 329 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 507 CCTGG ^CC[AT]GG MaeI 13 CTAG C^TAG MluNI 128 TGGCCA TGG^CCA MroI 494 TCCGGA T^CCGGA MscI 128 TGGCCA TGG^CCA MspA1I 663 CAGCTG C[AC]G^C[GT]G MvaI 507 CCTGG CC^[AT]GG NaeI 588 GCCGGC GCC^GGC NgoMIV 588 GCCGGC G^CCGGC PflMI 236 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 503 AGGTCCT [GA]G^G[AT]CC[CT] PstI 58 CTGCAG CTGCA^G PvuII 663 CAGCTG CAG^CTG SacI 46 GAGCTC GAGCT^C SapI 64 GAAGAGC GCTCTTC(1/4) ScaI 566 AGTACT AGT^ACT ScrFI 507 CCTGG CC^[ACGT]GG SfcI 58 CTGCAG C^T[GA][CT]AG SspBI 440 TGTACA T^GTACA TfiI 80 GATTC G^A[AT]TC TspRI 92 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 236 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbeI 585 GGCGCC GGCGC^C 591 GGCGCC BsiEI 7 CGGCCG CG[GA][CT]^CG 71 CGGTCG BsrI 93 ACTGG ACTGG(1/-1) 119 ACTGG DraII 139 GGGCCCC [GA]G^G[ACGT]CC[CT] 503 AGGTCCT EarI 613 CTCTTC CTCTTC(1/4) 64 GAAGAG EcoO109I 139 GGGCCCC [GA]G^G[ACGT]CC[CT] 503 AGGTCCT EcoT14I 515 CCAAGG C^C[AT][AT]GG 581 CCAAGG HgaI 427 GACGC GACGC(5/10) 523 GACGC KasI 585 GGCGCC G^GCGCC 591 GGCGCC Ksp632I 613 CTCTTC CTCTTC(1/4) 64 GAAGAG MaeIII 88 GTGAC ^GT[ACGT]AC 533 GTGAC NarI 585 GGCGCC GG^CGCC 591 GGCGCC PleI 688 GACTC GAGTC(4/5) 703 GAGTC SfoI 585 GGCGCC GGC^GCC 591 GGCGCC SgrAI 296 CGCCGGTG C[GA]^CCGG[CT]G 587 CGCCGGCG SmlI 49 CTCAAG C^T[CT][GA]AG 184 CTCAAG StyI 515 CCAAGG C^C[AT][AT]GG 581 CCAAGG TaqI 74 TCGA T^CGA 197 TCGA Tsp45I 88 GTGAC ^GT[GC]AC 533 GTGAC TthHB8I 74 TCGA T^CGA 197 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BanII BbvI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BstF5I BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MspI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I TseI