(Circular) (Six-base) MAPSORT of: pFN21AE4535.seq Check: 5655 from: 1 to: 6511 pFN21AE4535 6511 bp With 182 enzymes: SgfI * March 28, 2012 14:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2206 3854 1937 Size: 269 1648 4594 Fragments arranged by size: 4594 1648 269 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 2816 4638 5011 1563 Size: 1253 1822 373 3063 Fragments arranged by size: 3063 1822 1253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2597 2679 3082 4574 5958 6226 1632 Size: 477 488 82 403 1492 1384 268 1917 Fragments arranged by size: 1917 1492 1384 488 477 403 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 2464 5410 6387 1203 Size: 1261 2946 977 1327 Fragments arranged by size: 2946 1327 1261 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 5240 5240 Size: 6511 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2296 1184 Size: 1112 5399 AlwNI CAG_nnn'CTG Cuts at: 2233 4254 5826 2233 Size: 2021 1572 2918 Fragments arranged by size: 2918 2021 1572 ApaLI G'TGCA_C Cuts at: 4568 5724 4568 Size: 1156 5355 ApoI r'AATT_y Cuts at: 1493 2178 2275 2901 3134 3416 3471 3505 3820 Size: 685 97 626 233 282 55 34 315 Cuts at: 3820 4046 4198 6307 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 685 626 315 282 233 226 152 97 55 34 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2490 3836 1870 Size: 73 547 1346 4545 Fragments arranged by size: 4545 1346 547 73 AvrII C'CTAG_G Cuts at: 2539 2539 Size: 6511 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3100 3133 3100 Size: 33 6478 BamHI G'GATC_C Cuts at: 2762 3841 4208 2762 Size: 1079 367 5065 Fragments arranged by size: 5065 1079 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 3832 5287 618 Size: 325 217 627 747 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 747 627 325 217 BanII G_rGCy'C Cuts at: 1391 3830 1391 Size: 2439 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 3556 928 Size: 971 465 1192 3883 Fragments arranged by size: 3883 1192 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4702 4736 1903 Size: 34 50 34 2681 34 3678 Fragments arranged by size: 3678 2681 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4403 5613 4403 Size: 1210 5301 BclI T'GATC_A Cuts at: 1548 2596 3223 1548 Size: 1048 627 4836 Fragments arranged by size: 4836 1048 627 BfrBI ATG'CAT Cuts at: 2008 2031 3400 2008 Size: 23 1369 5119 Fragments arranged by size: 5119 1369 23 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5122 136 Size: 107 122 71 631 605 3450 1525 Fragments arranged by size: 3450 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6506 6506 Size: 6511 BlpI GC'TnA_GC Cuts at: 3928 3928 Size: 6511 Bme1580I G_kGCm'C Cuts at: 1790 2539 4572 5728 1790 Size: 749 2033 1156 2573 Fragments arranged by size: 2573 2033 1156 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6511 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3223 4242 5200 Size: 416 53 83 399 24 1806 1019 958 Cuts at: 5200 442 Size: 1753 Fragments arranged by size: 1806 1753 1019 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 5171 1239 Size: 1320 2612 2579 Fragments arranged by size: 2612 2579 1320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4636 5501 5799 4636 Size: 865 298 5348 Fragments arranged by size: 5348 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3223 3812 5174 882 Size: 617 1724 589 1362 2219 Fragments arranged by size: 2219 1724 1362 617 589 BsaAI yAC'GTr Cuts at: 493 1204 3632 4302 493 Size: 711 2428 670 2702 Fragments arranged by size: 2702 2428 711 670 BsaBI GATnn'nnATC Cuts at: 6505 6505 Size: 6511 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4318 4700 5335 275 Size: 53 83 186 936 2785 382 635 1451 Fragments arranged by size: 2785 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4944 5342 5616 5763 6174 4944 Size: 398 274 147 411 5281 Fragments arranged by size: 5281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3347 3377 1178 Size: 30 165 30 1944 30 4312 Fragments arranged by size: 4312 1944 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6511 BseYI C'CCAG_C Cuts at: 5714 6210 5714 Size: 496 6015 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2703 1163 Size: 573 967 4971 Fragments arranged by size: 4971 967 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2967 3961 4722 5750 1540 Size: 275 186 966 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 966 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3091 3637 4572 4657 5728 3091 Size: 546 935 85 1071 3874 Fragments arranged by size: 3874 1071 935 546 85 BsmI GAATG_Cn' Cuts at: 2263 4022 4115 2263 Size: 1759 93 4659 Fragments arranged by size: 4659 1759 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2539 3091 3637 3830 4572 4657 5728 Size: 399 749 552 546 193 742 85 1071 Cuts at: 5728 1391 Size: 2174 Fragments arranged by size: 2174 1071 749 742 552 546 399 193 85 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 4400 6130 4400 Size: 1730 4781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3866 844 Size: 3022 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4398 1394 Size: 429 2575 3507 Fragments arranged by size: 3507 2575 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5006 5180 65 Size: 1183 3758 174 1396 Fragments arranged by size: 3758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5155 5342 1700 Size: 110 3345 187 2869 Fragments arranged by size: 3345 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6511 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 3509 794 Size: 2715 3796 BstBI TT'CG_AA Cuts at: 5315 5315 Size: 6511 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2762 2890 3482 3841 4208 4593 4610 6062 Size: 790 128 592 359 367 385 17 1452 Cuts at: 6062 6148 6160 6506 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 790 592 385 367 359 346 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2568 2568 Size: 6511 BtgI C'CryG_G Cuts at: 513 1065 2317 2967 3374 513 Size: 552 1252 650 407 3650 Fragments arranged by size: 3650 1252 650 552 407 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3716 487 Size: 683 56 2490 3282 Fragments arranged by size: 3282 2490 683 56 BtsI GCAGTG_nn' Cuts at: 4027 4821 4841 4027 Size: 794 20 5697 Fragments arranged by size: 5697 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 3025 3150 3815 3025 Size: 125 665 5721 Fragments arranged by size: 5721 665 125 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2242 2944 5518 809 Size: 507 926 702 2574 1802 Fragments arranged by size: 2574 1802 926 702 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3377 3958 4847 Size: 54 1425 50 275 18 1547 581 889 Cuts at: 4847 6476 8 Size: 1629 43 Fragments arranged by size: 1629 1547 1425 889 581 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 3439 4441 1353 Size: 1263 823 1002 3423 Fragments arranged by size: 3423 1263 1002 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5098 5472 5618 387 Size: 1064 138 3509 374 146 1280 Fragments arranged by size: 3509 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2559 2597 2679 3082 4574 5171 Size: 393 477 450 38 82 403 1492 597 Cuts at: 5171 5958 6226 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1492 787 597 477 450 403 393 268 82 38 EcoRI G'AATT_C Cuts at: 2178 3820 4198 2178 Size: 1642 378 4491 Fragments arranged by size: 4491 1642 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2921 2921 Size: 6511 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 5658 1678 Size: 3980 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5152 5184 5226 5258 1373 Size: 32 135 32 3580 32 42 32 2626 Fragments arranged by size: 3580 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 4101 669 Size: 988 281 1671 246 246 3079 Fragments arranged by size: 3079 1671 988 281 246 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6511 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2987 5625 5809 1305 Size: 1682 2638 184 2007 Fragments arranged by size: 2638 2007 1682 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3379 10 Size: 54 1425 343 1547 3142 Fragments arranged by size: 3142 1547 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 2990 3405 4469 4828 4987 518 Size: 648 1303 521 415 1064 359 159 2042 Fragments arranged by size: 2042 1303 1064 648 521 415 359 159 MspA1I CmG'CkG Cuts at: 1606 3927 4604 5752 5997 1606 Size: 2321 677 1148 245 2120 Fragments arranged by size: 2321 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2317 513 Size: 552 1252 4707 Fragments arranged by size: 4707 1252 552 NdeI CA'TA_TG Cuts at: 387 2027 3284 387 Size: 1640 1257 3614 Fragments arranged by size: 3614 1640 1257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NsiI A_TGCA'T Cuts at: 2010 2033 3402 2010 Size: 23 1369 5119 Fragments arranged by size: 5119 1369 23 NspI r_CATG'y Cuts at: 1132 1229 2068 2468 2995 3869 5414 1132 Size: 97 839 400 527 874 1545 2229 Fragments arranged by size: 2229 1545 874 839 527 400 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6511 PciI A'CATG_T Cuts at: 2464 5410 2464 Size: 2946 3565 PflMI CCAn_nnn'nTGG Cuts at: 1108 2201 3040 1108 Size: 1093 839 4579 Fragments arranged by size: 4579 1093 839 PfoI T'CCnGG_A Cuts at: 2719 3163 2719 Size: 444 6067 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 3276 4081 3276 Size: 805 5706 PstI C_TGCA'G Cuts at: 830 3863 830 Size: 3033 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SapI GCTCTTCn'nnn_ Cuts at: 2616 2616 Size: 6511 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 4759 6471 4759 Size: 1712 4799 SfcI C'TryA_G Cuts at: 826 1045 2524 3859 4994 5675 5866 826 Size: 219 1479 1335 1135 681 191 1471 Fragments arranged by size: 1479 1471 1335 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 3838 1872 Size: 1966 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4615 5516 5778 820 Size: 197 926 2672 901 262 1553 Fragments arranged by size: 2672 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 2068 3869 1132 Size: 936 1801 3774 Fragments arranged by size: 3774 1801 936 SspI AAT'ATT Cuts at: 5 52 3156 3694 4435 5 Size: 47 3104 538 741 2081 Fragments arranged by size: 3104 2081 741 538 47 StuI AGG'CCT Cuts at: 1517 3251 1517 Size: 1734 4777 StyI C'CwwG_G Cuts at: 513 1065 2317 2539 2777 3252 3294 3950 513 Size: 552 1252 222 238 475 42 656 3074 Fragments arranged by size: 3074 1252 656 552 475 238 222 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2522 3035 3245 4538 4555 4708 4893 Size: 1255 942 513 210 1293 17 153 185 Cuts at: 4893 5338 325 Size: 445 1498 Fragments arranged by size: 1498 1293 1255 942 513 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4757 6469 96 Size: 274 80 33 51 1443 2780 1712 138 Fragments arranged by size: 2780 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2416 2448 3347 3379 6471 6503 2416 Size: 32 899 32 3092 32 2424 Fragments arranged by size: 3092 2424 899 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 2846 3847 2846 Size: 1001 5510 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6511 XmaI C'CCGG_G Cuts at: 1870 3836 1870 Size: 1966 4545 XmnI GAAnn'nnTTC Cuts at: 2728 2904 4640 2728 Size: 176 1736 4599 Fragments arranged by size: 4599 1736 176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI