################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 7 CGCG CG^CG AcsI 486 GAATTC [GA]^AATT[CT] AocI 43 CCTGAGG CC^T[ACGT]AGG ApoI 486 GAATTC [GA]^AATT[CT] Asp700I 215 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 3 GGACC G^G[AT]CC BbsI 291 GAAGAC GAAGAC(2/6) BbvCI 329 CCTCAGC CCTCAGC(-5/-2) BpuAI 291 GAAGAC GAAGAC(2/6) BsaI 85 GAGACC GGTCTC(1/5) BseRI 100 GAGGAG GAGGAG(10/8) BsmFI 40 GTCCC GGGAC(10/14) BsrDI 246 CATTGC GCAATG(2/0) BsrI 37 CCAGT ACTGG(1/-1) BssKI 312 CCAGG ^CC[ACGT]GG BstNI 312 CCAGG CC^[AT]GG BstUI 7 CGCG CG^CG BstXI 365 CCAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 43 CCTGAGG CC^T[ACGT]AGG Cfr13I 3 GGACC G^G[ACGT]CC DraI 400 TTTAAA TTT^AAA Eco81I 43 CCTGAGG CC^T[ACGT]AGG EcoNI 43 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 486 GAATTC G^AATTC EcoRII 312 CCAGG ^CC[AT]GG HaeIII 327 GGCC GG^CC KspI 6 CCGCGG CCGC^GG MaeII 266 ACGT A^CGT MaeIII 268 GTGAC ^GT[ACGT]AC MvaI 312 CCAGG CC^[AT]GG MvnI 7 CGCG CG^CG PflMI 251 CCAAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 153 GACTC GAGTC(4/5) PvuII 332 CAGCTG CAG^CTG SacII 6 CCGCGG CCGC^GG SapI 109 GAAGAGC GCTCTTC(1/4) Sau96I 3 GGACC G^G[ACGT]CC ScrFI 312 CCAGG CC^[ACGT]GG SfaNI 186 GCATC GCATC(5/9) SfcI 226 CTACAG C^T[GA][CT]AG SmlI 161 CTCAAG C^T[CT][GA]AG StuI 326 AGGCCT AGG^CCT TaqI 422 TCGA T^CGA TfiI 215 GAATC G^A[AT]TC Tsp45I 268 GTGAC ^GT[GC]AC TspRI 75 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 422 TCGA T^CGA Van91I 251 CCAAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 215 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 38 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 151 CAGACTCTG AspI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 153 GACTCTGTC BfaI 190 CTAG C^TAG 503 CTAG BsmAI 85 GAGAC GTCTC(1/5) 159 GTCTC BstAPI 15 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 274 GCAACATCTGC Cac8I 11 GCGAGC GC[ACGT]^[ACGT]GC 456 GCCTGC DpnII 72 GATC ^GATC 198 GATC DsaI 6 CCGCGG C^C[GA][CT]GG 515 CCACGG HinfI 153 GACTC G^A[ACGT]TC 215 GAATC HphI 466 GGTGA GGTGA(8/7) 362 TCACC MaeI 190 CTAG C^TAG 503 CTAG MboI 72 GATC ^GATC 198 GATC MseI 401 TTAA T^TAA 430 TTAA MspA1I 6 CCGCGG C[AC]G^C[GT]G 332 CAGCTG NdeII 72 GATC ^GATC 198 GATC NlaIV 448 GGAACC GG[ACGT]^[ACGT]CC 519 GGAGCC PflFI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 153 GACTCTGTC Sau3AI 72 GATC ^GATC 198 GATC Tru9I 401 TTAA T^TAA 430 TTAA Tsp509I 220 AATT ^AATT 487 AATT Tth111I 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 153 GACTCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI DdeI EarI Fnu4HI ItaI Ksp632I MboII MnlI MwoI TseI