(Circular) (Six-base) MAPSORT of: pFN21AB6238.seq Check: 3026 from: 1 to: 6736 pFN21AB6238 6736 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2391 4546 278 Size: 53 83 186 936 855 2155 2468 Fragments arranged by size: 2468 2155 936 855 186 83 53 AccI GT'mk_AC Cuts at: 1937 4079 1937 Size: 2142 4594 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 4863 5236 1563 Size: 3300 373 3063 Fragments arranged by size: 3300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2502 2519 2558 2648 3545 4799 6183 Size: 413 457 17 39 90 897 1254 1384 Cuts at: 6183 6451 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1254 897 457 413 268 90 39 17 AfeI AGC'GCT Cuts at: 2868 2868 Size: 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 5635 6612 1203 Size: 4432 977 1327 Fragments arranged by size: 4432 1327 977 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6736 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2057 2757 1184 Size: 873 700 5163 Fragments arranged by size: 5163 873 700 AlwNI CAG_nnn'CTG Cuts at: 2770 3562 3664 4479 6051 2770 Size: 792 102 815 1572 3455 Fragments arranged by size: 3455 1572 815 792 102 ApaI G_GGCC'C Cuts at: 2617 3375 3409 2617 Size: 758 34 5944 Fragments arranged by size: 5944 758 34 ApaLI G'TGCA_C Cuts at: 4793 5949 4793 Size: 1156 5580 ApoI r'AATT_y Cuts at: 1493 3356 4045 4271 4423 6532 1493 Size: 1863 689 226 152 2109 1697 Fragments arranged by size: 2109 1863 1697 689 226 152 AseI AT'TA_AT Cuts at: 160 1032 5290 160 Size: 872 4258 1606 Fragments arranged by size: 4258 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 2373 2441 3402 4061 1870 Size: 73 430 68 961 659 4545 Fragments arranged by size: 4545 961 659 430 73 68 BamHI G'GATC_C Cuts at: 3210 4066 4433 3210 Size: 856 367 5513 Fragments arranged by size: 5513 856 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 2593 4057 5512 618 Size: 325 217 627 590 216 1464 1455 1842 Fragments arranged by size: 1842 1464 1455 627 590 325 217 216 BanII G_rGCy'C Cuts at: 1391 2617 2705 3375 3409 4055 1391 Size: 1226 88 670 34 646 4072 Fragments arranged by size: 4072 1226 670 646 88 34 BbeI G_GCGC'C Cuts at: 2597 2597 Size: 6736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2532 3110 928 Size: 971 633 578 4554 Fragments arranged by size: 4554 971 633 578 BbvCI CC'TCA_GC Cuts at: 2010 3671 4023 2010 Size: 1661 352 4723 Fragments arranged by size: 4723 1661 352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4927 4961 1903 Size: 34 2990 34 3678 Fragments arranged by size: 3678 2990 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4628 5838 4628 Size: 1210 5526 BclI T'GATC_A Cuts at: 1548 1548 Size: 6736 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2720 3350 5347 Size: 107 122 71 631 605 1048 630 1997 Cuts at: 5347 136 Size: 1525 Fragments arranged by size: 1997 1525 1048 631 630 605 122 107 71 BglII A'GATC_T Cuts at: 3021 6731 3021 Size: 3710 3026 BlpI GC'TnA_GC Cuts at: 2030 2411 3147 3239 3426 4153 2030 Size: 381 736 92 187 727 4613 Fragments arranged by size: 4613 736 727 381 187 92 Bme1580I G_kGCm'C Cuts at: 1790 2382 2617 2989 3375 3409 3931 4797 5953 Size: 592 235 372 386 34 522 866 1156 Cuts at: 5953 1790 Size: 2573 Fragments arranged by size: 2573 1156 866 592 522 386 372 235 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6736 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2992 4467 5425 Size: 416 53 83 399 24 1575 1475 958 Cuts at: 5425 442 Size: 1753 Fragments arranged by size: 1753 1575 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 3622 3867 3954 3960 5396 1239 Size: 1388 995 245 87 6 1436 2579 Fragments arranged by size: 2579 1436 1388 995 245 87 6 Bpu10I CC'TnA_GC Cuts at: 2010 3671 3691 4023 2010 Size: 1661 20 332 4723 Fragments arranged by size: 4723 1661 332 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4861 5726 6024 4861 Size: 865 298 5573 Fragments arranged by size: 5573 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 3763 3835 5399 882 Size: 617 600 1664 72 1564 2219 Fragments arranged by size: 2219 1664 1564 617 600 72 BsaAI yAC'GTr Cuts at: 493 1204 4527 493 Size: 711 3323 2702 Fragments arranged by size: 3323 2702 711 BsaBI GATnn'nnATC Cuts at: 6730 6730 Size: 6736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 2594 3107 3342 Size: 53 83 186 936 855 206 513 235 Cuts at: 3342 4543 4925 5560 275 Size: 1201 382 635 1451 Fragments arranged by size: 1451 1201 936 855 635 513 382 235 206 186 83 53 BsaWI w'CCGG_w Cuts at: 3252 4033 5169 5567 5841 5988 6399 3252 Size: 781 1136 398 274 147 411 3589 Fragments arranged by size: 3589 1136 781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4001 4031 1178 Size: 30 165 30 2598 30 3883 Fragments arranged by size: 3883 2598 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2117 2342 3368 3594 1186 Size: 841 90 225 1026 226 4328 Fragments arranged by size: 4328 1026 841 226 225 90 BseYI C'CCAG_C Cuts at: 2455 3090 3151 3654 3731 3782 3953 4002 5939 Size: 635 61 503 77 51 171 49 1937 Cuts at: 5939 6435 2455 Size: 496 2756 Fragments arranged by size: 2756 1937 635 503 496 171 77 61 51 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3293 3555 3758 1163 Size: 573 1557 262 203 4141 Fragments arranged by size: 4141 1557 573 262 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4186 4947 5975 1540 Size: 275 186 2185 761 1028 2301 Fragments arranged by size: 2301 2185 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3301 4797 4882 5953 3301 Size: 1496 85 1071 4084 Fragments arranged by size: 4084 1496 1071 85 BsmI GAATG_Cn' Cuts at: 4247 4340 4247 Size: 93 6643 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2617 2705 2989 3301 3375 3409 Size: 399 592 235 88 284 312 74 34 Cuts at: 3409 3931 4055 4797 4882 5953 1391 Size: 522 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 522 399 312 284 235 124 88 85 74 34 BspEI T'CCGG_A Cuts at: 6399 6399 Size: 6736 BspHI T'CATG_A Cuts at: 4625 6355 4625 Size: 1730 5006 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2942 3421 3649 4091 844 Size: 1273 825 479 228 442 3489 Fragments arranged by size: 3489 1273 825 479 442 228 BsrBI CCG'CTC Cuts at: 1394 1823 3208 3578 4623 1394 Size: 429 1385 370 1045 3507 Fragments arranged by size: 3507 1385 1045 429 370 BsrDI GCAATG_nn' Cuts at: 65 1248 2739 5231 5405 65 Size: 1183 1491 2492 174 1396 Fragments arranged by size: 2492 1491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5380 5567 1700 Size: 110 3570 187 2869 Fragments arranged by size: 3570 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 2645 3573 4796 5808 2645 Size: 928 1223 1012 3573 Fragments arranged by size: 3573 1223 1012 928 BstAPI GCAn_nnn'nTGC Cuts at: 794 2162 794 Size: 1368 5368 BstBI TT'CG_AA Cuts at: 5540 5540 Size: 6736 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6736 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2065 Size: 6736 BstYI r'GATC_y Cuts at: 1972 3021 3210 4066 4433 4818 4835 6287 6373 Size: 1049 189 856 367 385 17 1452 86 Cuts at: 6373 6385 6731 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1049 856 385 367 346 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2745 2774 513 Size: 552 939 75 666 29 4475 Fragments arranged by size: 4475 939 666 552 75 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2071 3118 3526 487 Size: 683 56 845 1047 408 3697 Fragments arranged by size: 3697 1047 845 683 408 56 BtsI GCAGTG_nn' Cuts at: 3223 3786 3931 4252 5046 5066 3223 Size: 563 145 321 794 20 4893 Fragments arranged by size: 4893 794 563 321 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 4040 4040 Size: 6736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3565 5743 809 Size: 507 2249 2178 1802 Fragments arranged by size: 2249 2178 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4183 5072 6701 Size: 54 1425 50 275 18 2353 889 1629 Cuts at: 6701 8 Size: 43 Fragments arranged by size: 2353 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4183 1537 Size: 275 2371 4090 Fragments arranged by size: 4090 2371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4666 1353 Size: 3313 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 5323 5697 5843 387 Size: 1064 138 1149 2585 374 146 1280 Fragments arranged by size: 2585 1280 1149 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2502 2519 2558 2627 2648 3545 Size: 393 413 457 17 39 69 21 897 Cuts at: 3545 3622 3867 3954 3960 4799 5396 6183 6451 Size: 77 245 87 6 839 597 787 268 Cuts at: 6451 1239 Size: 1524 Fragments arranged by size: 1524 897 839 787 597 457 413 393 268 245 87 77 69 39 21 17 6 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 6736 EcoO109I rG'GnC_Cy Cuts at: 2613 2614 3363 3372 3490 3661 3863 2613 Size: 1 749 9 118 171 202 5486 Fragments arranged by size: 5486 749 202 171 118 9 1 EcoRI G'AATT_C Cuts at: 3356 4045 4423 3356 Size: 689 378 5669 Fragments arranged by size: 5669 689 378 FspI TGC'GCA Cuts at: 5242 5242 Size: 6736 HaeII r_GCGC'y Cuts at: 1678 2597 2870 3645 5883 1678 Size: 919 273 775 2238 2531 Fragments arranged by size: 2531 2238 919 775 273 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5377 5409 5451 5483 1373 Size: 32 135 32 3805 32 42 32 2626 Fragments arranged by size: 3805 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3436 4080 4326 669 Size: 988 281 1498 644 246 3079 Fragments arranged by size: 3079 1498 988 644 281 246 HindIII A'AGCT_T Cuts at: 748 2027 748 Size: 1279 5457 HpaI GTT'AAC Cuts at: 4326 4326 Size: 6736 KasI G'GCGC_C Cuts at: 2593 2593 Size: 6736 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 4335 4335 Size: 6736 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3632 5850 6034 1305 Size: 2327 2218 184 2007 Fragments arranged by size: 2327 2218 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4914 Fragments arranged by size: 4914 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2180 4694 5053 5212 518 Size: 648 897 117 2514 359 159 2042 Fragments arranged by size: 2514 2042 897 648 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2116 2924 3921 4152 4829 5977 6222 1606 Size: 510 808 997 231 677 1148 245 2120 Fragments arranged by size: 2120 1148 997 808 677 510 245 231 NarI GG'CG_CC Cuts at: 2594 2594 Size: 6736 NcoI C'CATG_G Cuts at: 513 1065 2004 2079 513 Size: 552 939 75 5170 Fragments arranged by size: 5170 939 552 75 NdeI CA'TA_TG Cuts at: 387 387 Size: 6736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NspI r_CATG'y Cuts at: 1132 1229 4094 5639 1132 Size: 97 2865 1545 2229 Fragments arranged by size: 2865 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6736 PciI A'CATG_T Cuts at: 5635 5635 Size: 6736 PflMI CCAn_nnn'nTGG Cuts at: 1108 2255 3090 3500 3562 1108 Size: 1147 835 410 62 4282 Fragments arranged by size: 4282 1147 835 410 62 PfoI T'CCnGG_A Cuts at: 3062 3062 Size: 6736 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3363 3661 3363 Size: 298 6438 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6736 PsiI TTA'TAA Cuts at: 4306 4306 Size: 6736 PspOMI G'GGCC_C Cuts at: 2613 3371 3405 2613 Size: 758 34 5944 Fragments arranged by size: 5944 758 34 PstI C_TGCA'G Cuts at: 830 3195 3423 4088 830 Size: 2365 228 665 3478 Fragments arranged by size: 3478 2365 665 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6736 PvuII CAG'CTG Cuts at: 2116 2924 2116 Size: 808 5928 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SanDI GG'GwC_CC Cuts at: 3363 3363 Size: 6736 SbfI CC_TGCA'GG Cuts at: 4088 4088 Size: 6736 ScaI AGT'ACT Cuts at: 4984 6696 4984 Size: 1712 5024 SfcI C'TryA_G Cuts at: 826 1045 3191 3419 4084 5219 5900 6091 826 Size: 219 2146 228 665 1135 681 191 1471 Fragments arranged by size: 2146 1471 1135 681 665 228 219 191 SfoI GGC'GCC Cuts at: 2595 2595 Size: 6736 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 2375 4063 1872 Size: 503 1688 4545 Fragments arranged by size: 4545 1688 503 SmlI C'TyrA_G Cuts at: 820 1017 1943 4840 5741 6003 820 Size: 197 926 2897 901 262 1553 Fragments arranged by size: 2897 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4527 493 Size: 4034 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6736 SphI G_CATG'C Cuts at: 1132 4094 1132 Size: 2962 3774 SspI AAT'ATT Cuts at: 5 52 4660 5 Size: 47 4608 2081 Fragments arranged by size: 4608 2081 47 StuI AGG'CCT Cuts at: 1517 2693 1517 Size: 1176 5560 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 3367 4175 513 Size: 552 939 75 1288 808 3074 Fragments arranged by size: 3074 1288 939 808 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3082 3668 4763 4780 4933 5118 5563 Size: 1255 1502 586 1095 17 153 185 445 Cuts at: 5563 325 Size: 1498 Fragments arranged by size: 1502 1498 1255 1095 586 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3819 4982 6694 Size: 274 80 33 51 1443 1842 1163 1712 Cuts at: 6694 96 Size: 138 Fragments arranged by size: 1842 1712 1443 1163 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3202 3234 3537 3569 3859 3891 6696 6728 3202 Size: 32 303 32 290 32 2805 32 3210 Fragments arranged by size: 3210 2805 303 290 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 2893 4072 2893 Size: 1179 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 3091 3139 3751 2065 Size: 1026 48 612 5050 Fragments arranged by size: 5050 1026 612 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 2373 4061 1870 Size: 503 1688 4545 Fragments arranged by size: 4545 1688 503 XmnI GAAnn'nnTTC Cuts at: 4865 4865 Size: 6736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2389 4544 276 Size: 53 83 186 936 855 2155 2468 Fragments arranged by size: 2468 2155 936 855 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI