################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 138 GTCTAC GT^[AC][GT]AC AclI 936 AACGTT AA^CGTT AcsI 542 AAATTC [GA]^AATT[CT] AfaI 554 GTAC GT^AC AlwNI 1043 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 542 AAATTC [GA]^AATT[CT] AseI 558 ATTAAT AT^TAAT AsnI 558 ATTAAT AT^TAAT AvaI 442 CTCGAG C^[CT]CG[GA]G BanI 943 GGTGCC G^G[CT][GA]CC BciVI 798 GTATCC GTATCC(6/5) BmyI 994 GTGCCC G[AGT]GC[ACT]^C BsaI 296 GGTCTC GGTCTC(1/5) BsgI 592 CTGCAC GTGCAG(16/14) BsiYI 233 CCAATCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 233 CCAATCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 751 GGGAC GGGAC(10/14) BsoBI 442 CTCGAG C^[CT]CG[GA]G Bsp1286I 994 GTGCCC G[AGT]GC[ACT]^C BspMI 590 ACCTGC ACCTGC(4/8) BsrI 216 CCAGT ACTGG(1/-1) BstAPI 1042 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 205 GATC ^GATC DraII 241 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 1213 GAAGAG CTCTTC(1/4) EcoO109I 241 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 69 ATGCAT ATGCA^T HincII 219 GTTAAC GT[CT]^[GA]AC HindII 219 GTTAAC GT[CT]^[GA]AC HindIII 825 AAGCTT A^AGCTT HpaI 219 GTTAAC GTT^AAC Ksp632I 1213 GAAGAG CTCTTC(1/4) MaeII 937 ACGT A^CGT MboI 205 GATC ^GATC MslI 106 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1046 CAGCTG C[AC]G^C[GT]G NdeII 205 GATC ^GATC NsiI 69 ATGCAT ATGCA^T PaeR7I 442 CTCGAG C^TCGAG PpuMI 241 AGGACCC [GA]G^G[AT]CC[CT] PshBI 558 ATTAAT AT^TAAT Psp1406I 936 AACGTT AA^CGTT PvuII 1046 CAGCTG CAG^CTG RsaI 554 GTAC GT^AC Sau3AI 205 GATC ^GATC SexAI 57 ACCTGGT A^CC[AT]GGT SfaNI 846 GATGC GCATC(5/9) SpeI 1147 ACTAGT A^CTAGT SphI 1154 GCATGC GCATG^C SspI 166 AATATT AAT^ATT TfiI 369 GAATC G^A[AT]TC XhoI 442 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AflIII 308 ACATGT A^C[GA][CT]GT 794 ACATGT AvaII 242 GGACC G^G[AT]CC 516 GGACC BsaJI 245 CCCTGG C^C[ACGT][ACGT]GG 292 CCTCGG BspLU11I 308 ACATGT A^CATGT 794 ACATGT Cfr13I 242 GGACC G^G[ACGT]CC 516 GGACC DraI 177 TTTAAA TTT^AAA 780 TTTAAA MwoI 1042 GCAGCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1097 GCCTCACTAGC NlaIV 242 GGACCC GG[ACGT]^[ACGT]CC 943 GGTGCC PleI 753 GACTC GAGTC(4/5) 1236 GACTC Sau96I 242 GGACC G^G[ACGT]CC 516 GGACC SmlI 442 CTCGAG C^T[CT][GA]AG 822 CTCAAG TaqI 443 TCGA T^CGA 921 TCGA TthHB8I 443 TCGA T^CGA 921 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BbvI BfaI BsmAI BssKI BstF5I BstNI Cac8I DdeI EcoRII Fnu4HI FokI HinfI ItaI MaeI MaeIII MboII MnlI MseI MvaI NlaIII NspI ScrFI Tru9I TseI Tsp45I Tsp509I TspRI