(Circular) (Six-base) MAPSORT of: pFN21AE6328.seq Check: 1760 from: 1 to: 6025 pFN21AE6328 6025 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2144 3368 1937 Size: 207 1224 4594 Fragments arranged by size: 4594 1224 207 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 2942 4152 4525 1563 Size: 1379 1210 373 3063 Fragments arranged by size: 3063 1379 1210 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2741 4088 5472 5740 1632 Size: 1109 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 1109 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 2313 2799 4924 5901 1203 Size: 1110 486 2125 977 1327 Fragments arranged by size: 2125 1327 1110 977 486 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 2749 Size: 32 5993 AlwNI CAG_nnn'CTG Cuts at: 3053 3768 5340 3053 Size: 715 1572 3738 Fragments arranged by size: 3738 1572 715 ApaLI G'TGCA_C Cuts at: 4082 5238 4082 Size: 1156 4869 ApoI r'AATT_y Cuts at: 1493 2547 3334 3560 3712 5821 1493 Size: 1054 787 226 152 2109 1697 Fragments arranged by size: 2109 1697 1054 787 226 152 AseI AT'TA_AT Cuts at: 160 1032 2564 4579 160 Size: 872 1532 2015 1606 Fragments arranged by size: 2015 1606 1532 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2447 3350 1870 Size: 73 504 903 4545 Fragments arranged by size: 4545 903 504 73 BamHI G'GATC_C Cuts at: 3355 3722 3355 Size: 367 5658 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2948 3346 4801 618 Size: 325 217 627 1161 398 1455 1842 Fragments arranged by size: 1842 1455 1161 627 398 325 217 BanII G_rGCy'C Cuts at: 1391 3344 1391 Size: 1953 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2814 3917 5127 2814 Size: 1103 1210 3712 Fragments arranged by size: 3712 1210 1103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BfrBI ATG'CAT Cuts at: 2076 2076 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 3003 4086 5242 1790 Size: 1213 1083 1156 2573 Fragments arranged by size: 2573 1213 1156 1083 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4685 1239 Size: 3446 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2812 4150 5015 5313 2812 Size: 1338 865 298 3524 Fragments arranged by size: 3524 1338 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 4688 882 Size: 617 808 2381 2219 Fragments arranged by size: 2381 2219 808 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3832 4214 4849 275 Size: 53 83 186 936 2299 382 635 1451 Fragments arranged by size: 2299 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4458 4856 5130 5277 5688 4458 Size: 398 274 147 411 4795 Fragments arranged by size: 4795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 1178 Size: 30 165 30 1083 30 4687 Fragments arranged by size: 4687 1083 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6025 BseYI C'CCAG_C Cuts at: 5228 5724 5228 Size: 496 5529 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 1163 Size: 573 846 4606 Fragments arranged by size: 4606 846 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4086 4171 5242 4086 Size: 85 1071 4869 Fragments arranged by size: 4869 1071 85 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3003 3344 4086 4171 5242 1391 Size: 399 1213 341 742 85 1071 2174 Fragments arranged by size: 2174 1213 1071 742 399 341 85 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 3380 844 Size: 1760 776 3489 Fragments arranged by size: 3489 1760 776 BsrBI CCG'CTC Cuts at: 1394 1823 3912 1394 Size: 429 2089 3507 Fragments arranged by size: 3507 2089 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4669 4856 1700 Size: 110 2859 187 2869 Fragments arranged by size: 2869 2859 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 3053 794 Size: 2259 3766 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstYI r'GATC_y Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020 Size: 1383 367 385 17 1452 86 12 346 Cuts at: 6020 1972 Size: 1977 Fragments arranged by size: 1977 1452 1383 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 3541 4335 4355 3541 Size: 794 20 5211 Fragments arranged by size: 5211 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 2184 2787 3329 2184 Size: 603 542 4880 Fragments arranged by size: 4880 603 542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3472 4361 5990 Size: 54 1425 50 275 18 1642 889 1629 Cuts at: 5990 8 Size: 43 Fragments arranged by size: 1642 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3213 3955 1353 Size: 1860 742 3423 Fragments arranged by size: 3423 1860 742 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 4088 4685 5472 5740 1239 Size: 393 1109 1347 597 787 268 1524 Fragments arranged by size: 1524 1347 1109 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2247 2247 Size: 6025 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 5172 1678 Size: 3494 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2743 2775 4666 4698 4740 Size: 32 135 32 1171 32 1891 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 1891 1171 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2226 3369 3615 669 Size: 988 281 288 1143 246 3079 Fragments arranged by size: 3079 1143 988 288 281 246 HindIII A'AGCT_T Cuts at: 748 2830 748 Size: 2082 3943 HpaI GTT'AAC Cuts at: 2226 3615 2226 Size: 1389 4636 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2513 5139 5323 1305 Size: 1208 2626 184 2007 Fragments arranged by size: 2626 2007 1208 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4203 Fragments arranged by size: 4203 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2115 3983 4342 4501 518 Size: 648 949 1868 359 159 2042 Fragments arranged by size: 2042 1868 949 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3053 3441 4118 5266 5511 1606 Size: 1447 388 677 1148 245 2120 Fragments arranged by size: 2120 1447 1148 677 388 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NsiI A_TGCA'T Cuts at: 2078 2078 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2120 2317 2407 2500 2803 3163 3383 Size: 97 891 197 90 93 303 360 220 Cuts at: 3383 4928 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 891 360 303 220 197 97 93 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 2313 2799 4924 2313 Size: 486 2125 3414 Fragments arranged by size: 3414 2125 486 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2247 Size: 6025 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 2196 3595 2196 Size: 1399 4626 PstI C_TGCA'G Cuts at: 830 3377 830 Size: 2547 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 3053 3053 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SexAI A'CCwGG_T Cuts at: 2062 2062 Size: 6025 SfcI C'TryA_G Cuts at: 826 1045 3373 4508 5189 5380 826 Size: 219 2328 1135 681 191 1471 Fragments arranged by size: 2328 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 2827 4129 5030 5292 820 Size: 197 926 504 380 1302 901 262 1553 Fragments arranged by size: 1553 1302 926 901 504 380 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 3152 152 Size: 3000 3025 SphI G_CATG'C Cuts at: 1132 3163 3383 1132 Size: 2031 220 3774 Fragments arranged by size: 3774 2031 220 SspI AAT'ATT Cuts at: 5 52 2173 3949 5 Size: 47 2121 1776 2081 Fragments arranged by size: 2121 2081 1776 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6025 StyI C'CwwG_G Cuts at: 513 1065 2004 3464 513 Size: 552 939 1460 3074 Fragments arranged by size: 3074 1460 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2289 3148 4052 4069 4222 4407 4852 Size: 1255 709 859 904 17 153 185 445 Cuts at: 4852 325 Size: 1498 Fragments arranged by size: 1498 1255 904 859 709 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2605 2637 5985 6017 2605 Size: 32 3348 32 2613 Fragments arranged by size: 3348 2613 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XhoI C'TCGA_G Cuts at: 1943 2447 1943 Size: 504 5521 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI