Restriction map for
pFN21AE1334
(Circular) (Six-base) MAPSORT of: pFN21AE1334.seq Check: 8207 from: 1 to: 5836
pFN21AE1334 5836 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2391 2762 3899 5283 5551 1632
Size: 543 216 371 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 543 371 268 216
AfeI AGC'GCT
Cuts at: 2565 2565
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 2036 820
Size: 197 1019 4620
Fragments arranged by size:
4620 1019 197
AflIII A'CryG_T
Cuts at: 1203 2934 4735 5712 1203
Size: 1731 1801 977 1327
Fragments arranged by size:
1801 1731 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2342 3145 3371 3523 5632 1493
Size: 849 803 226 152 2109 1697
Fragments arranged by size:
2109 1697 849 803 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 1870
Size: 73 1218 4545
Fragments arranged by size:
4545 1218 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2612 2645 2612
Size: 33 5803
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4612 618
Size: 325 217 627 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2963 3155 1391
Size: 1572 192 4072
Fragments arranged by size:
4072 1572 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 928
Size: 971 264 4601
Fragments arranged by size:
4601 971 264
BbvCI CC'TCA_GC
Cuts at: 2567 2567
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2376 5831 2376
Size: 3455 2381
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 3897 5053 1790
Size: 2107 1156 2573
Fragments arranged by size:
2573 2107 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 2802 2882 2962 4496 1239
Size: 1424 139 80 80 1534 2579
Fragments arranged by size:
2579 1534 1424 139 80 80
Bpu10I CC'TnA_GC
Cuts at: 2567 2567
Size: 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2522 3961 4826 5124 2306
Size: 216 1439 865 298 3018
Fragments arranged by size:
3018 1439 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2890 3627 493
Size: 711 1686 737 2702
Fragments arranged by size:
2702 1686 737 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 5039 5535 5039
Size: 496 5340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 3897 3982 5053 2595
Size: 1302 85 1071 3378
Fragments arranged by size:
3378 1302 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2595 2963 3155 3897 3982 5053 1391
Size: 399 805 368 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 805 742 399 368 192 85
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 2335 3725 5455 2335
Size: 1390 1730 2716
Fragments arranged by size:
2716 1730 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 3139 4331 4505 65
Size: 1183 1656 235 1192 174 1396
Fragments arranged by size:
1656 1396 1192 1183 235 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2554
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2376 3166 3533 3918 3935 5387 5473 5485
Size: 404 790 367 385 17 1452 86 12
Cuts at: 5485 5831 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 790 404 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2791 513
Size: 552 939 787 3558
Fragments arranged by size:
3558 939 787 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 3352 4146 4166 3352
Size: 794 20 5022
Fragments arranged by size:
5022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2935 2935
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3283 4172 5801
Size: 54 1425 50 275 18 1453 889 1629
Cuts at: 5801 8
Size: 43
Fragments arranged by size:
1629 1453 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2367 2526 2845 2923 3766 1353
Size: 762 252 159 319 78 843 3423
Fragments arranged by size:
3423 843 762 319 252 159 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2391 2663 2762 2802 2882 2962
Size: 393 543 216 272 99 40 80 80
Cuts at: 2962 3899 4496 5283 5551 1239
Size: 937 597 787 268 1524
Fragments arranged by size:
1524 937 787 597 543 393 272 268
216 99 80 80 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2298 2298
Size: 5836
EcoRI G'AATT_C
Cuts at: 2342 3145 3523 2342
Size: 803 378 4655
Fragments arranged by size:
4655 803 378
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2567 4983 1678
Size: 889 2416 2531
Fragments arranged by size:
2531 2416 889
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2543 2575 2582
Size: 32 135 32 662 32 277 32 7
Cuts at: 2582 2614 4477 4509 4551 4583 1373
Size: 32 1863 32 42 32 2626
Fragments arranged by size:
2626 1863 662 277 135 42 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2324 748
Size: 1576 4260
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 4950 5134 1305
Size: 720 2925 184 2007
Fragments arranged by size:
2925 2007 720 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4014
Fragments arranged by size:
4014 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3794 4153 4312 518
Size: 648 1320 1308 359 159 2042
Fragments arranged by size:
2042 1320 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3252 3929 5077 5322 1606
Size: 1646 677 1148 245 2120
Fragments arranged by size:
2120 1646 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 2426 387
Size: 2039 3797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 2991 3194 4739 1132
Size: 97 1762 203 1545 2229
Fragments arranged by size:
2229 1762 1545 203 97
PasI CC'CwG_GG
Cuts at: 1348 2053 1348
Size: 705 5131
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2640 2939 2640
Size: 299 5537
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PmlI CAC'GTG
Cuts at: 2890 2890
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2088 2120 2088
Size: 32 5804
PstI C_TGCA'G
Cuts at: 830 2780 3188 830
Size: 1950 408 3478
Fragments arranged by size:
3478 1950 408
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2923 2923
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2310 2515 2776 3184 4319 5000 5191
Size: 219 1265 205 261 408 1135 681 191
Cuts at: 5191 826
Size: 1471
Fragments arranged by size:
1471 1265 1135 681 408 261 219 205
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2321 2501 3940 4841 5103
Size: 197 926 93 285 180 1439 901 262
Cuts at: 5103 820
Size: 1553
Fragments arranged by size:
1553 1439 926 901 285 262 197 180
93
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 2138 3002 3760 5
Size: 47 2086 864 758 2081
Fragments arranged by size:
2086 2081 864 758 47
StuI AGG'CCT
Cuts at: 1517 2774 1517
Size: 1257 4579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2754 2826 3062 3275 513
Size: 552 939 750 72 236 213 3074
Fragments arranged by size:
3074 939 750 552 236 213 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2104 4082 5794
Size: 274 80 33 51 1443 127 1978 1712
Cuts at: 5794 96
Size: 138
Fragments arranged by size:
1978 1712 1443 274 138 127 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2921 2953 5796 5828 2921
Size: 32 2843 32 2929
Fragments arranged by size:
2929 2843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 2900 3030 3965 2900
Size: 130 935 4771
Fragments arranged by size:
4771 935 130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI