(Circular) (Six-base) MAPSORT of: pFN21AE1476.seq Check: 5356 from: 1 to: 6670 pFN21AE1476 6670 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 2525 3991 2525 Size: 1466 5204 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2873 2954 3206 3281 4733 6117 6385 1632 Size: 1241 81 252 75 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 1241 268 252 81 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AlwNI CAG_nnn'CTG Cuts at: 2492 2593 2831 4413 5985 2492 Size: 101 238 1582 1572 3177 Fragments arranged by size: 3177 1582 1572 238 101 ApaI G_GGCC'C Cuts at: 2996 3147 3416 2996 Size: 151 269 6250 Fragments arranged by size: 6250 269 151 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3598 3979 4205 4357 6466 1493 Size: 2105 381 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 381 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2520 3124 3416 3562 3995 1870 Size: 73 577 604 292 146 433 4545 Fragments arranged by size: 4545 604 577 433 292 146 73 BamHI G'GATC_C Cuts at: 3154 4000 4367 3154 Size: 846 367 5457 Fragments arranged by size: 5457 846 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2525 3991 5446 618 Size: 325 217 627 738 1466 1455 1842 Fragments arranged by size: 1842 1466 1455 738 627 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2996 3147 3416 3548 3569 3672 3989 Size: 699 906 151 269 132 21 103 317 Cuts at: 3989 1391 Size: 4072 Fragments arranged by size: 4072 906 699 317 269 151 132 103 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 3507 928 Size: 971 969 639 4091 Fragments arranged by size: 4091 971 969 639 BbvCI CC'TCA_GC Cuts at: 3867 3867 Size: 6670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2017 2035 2051 2069 2501 2535 4861 Size: 34 80 18 16 18 432 34 2326 Cuts at: 4861 4895 1903 Size: 34 3678 Fragments arranged by size: 3678 2326 432 80 34 34 34 18 18 16 BciVI GTATCCnnnnn_n' Cuts at: 3122 4562 5772 3122 Size: 1440 1210 4020 Fragments arranged by size: 4020 1440 1210 BclI T'GATC_A Cuts at: 1548 2601 1548 Size: 1053 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2409 5281 136 Size: 107 122 71 631 605 737 2872 1525 Fragments arranged by size: 2872 1525 737 631 605 122 107 71 BglII A'GATC_T Cuts at: 6665 6665 Size: 6670 BlpI GC'TnA_GC Cuts at: 2127 2845 3557 3713 4087 2127 Size: 718 712 156 374 4710 Fragments arranged by size: 4710 718 712 374 156 Bme1580I G_kGCm'C Cuts at: 1790 2440 2996 3147 3416 4731 5887 1790 Size: 650 556 151 269 1315 1156 2573 Fragments arranged by size: 2573 1315 1156 650 556 269 151 BmgBI CAC'GTC Cuts at: 1318 2866 1318 Size: 1548 5122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3153 4401 Size: 416 53 83 399 24 1553 183 1248 Cuts at: 4401 5359 442 Size: 958 1753 Fragments arranged by size: 1753 1553 1248 958 416 399 183 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2641 5330 1239 Size: 1402 2689 2579 Fragments arranged by size: 2689 2579 1402 Bpu10I CC'TnA_GC Cuts at: 2111 2703 3867 2111 Size: 592 1164 4914 Fragments arranged by size: 4914 1164 592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3614 4795 5660 5958 3614 Size: 1181 865 298 4326 Fragments arranged by size: 4326 1181 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 3303 6664 3303 Size: 3361 3309 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4477 4859 5494 275 Size: 53 83 186 936 2944 382 635 1451 Fragments arranged by size: 2944 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3114 3459 5103 5501 5775 5922 6333 3114 Size: 345 1644 398 274 147 411 3451 Fragments arranged by size: 3451 1644 411 398 345 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3519 3549 1178 Size: 30 165 30 2116 30 4299 Fragments arranged by size: 4299 2116 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3694 3848 1186 Size: 2508 154 4008 Fragments arranged by size: 4008 2508 154 BseYI C'CCAG_C Cuts at: 3432 3510 3548 3575 5873 6369 3432 Size: 78 38 27 2298 496 3733 Fragments arranged by size: 3733 2298 496 78 38 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3127 1163 Size: 573 1391 4706 Fragments arranged by size: 4706 1391 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4120 4881 5909 1540 Size: 275 186 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2521 2709 4731 4816 5887 2521 Size: 188 2022 85 1071 3304 Fragments arranged by size: 3304 2022 1071 188 85 BsmI GAATG_Cn' Cuts at: 2399 4181 4274 2399 Size: 1782 93 4795 Fragments arranged by size: 4795 1782 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2440 2521 2709 2996 3147 3416 Size: 399 300 350 81 188 287 151 269 Cuts at: 3416 3548 3569 3672 3989 4731 4816 5887 1391 Size: 132 21 103 317 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 317 300 287 269 188 151 132 103 85 81 21 BspEI T'CCGG_A Cuts at: 3114 6333 3114 Size: 3219 3451 BspHI T'CATG_A Cuts at: 4559 6289 4559 Size: 1730 4940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3757 3972 4025 844 Size: 2913 215 53 3489 Fragments arranged by size: 3489 2913 215 53 BsrBI CCG'CTC Cuts at: 1394 1823 3376 3605 4557 1394 Size: 429 1553 229 952 3507 Fragments arranged by size: 3507 1553 952 429 229 BsrDI GCAATG_nn' Cuts at: 65 1248 3244 3325 5165 5339 65 Size: 1183 1996 81 1840 174 1396 Fragments arranged by size: 1996 1840 1396 1183 174 81 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 3882 5314 5501 1700 Size: 110 376 1696 1432 187 2869 Fragments arranged by size: 2869 1696 1432 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 3371 4730 5742 3371 Size: 1359 1012 4299 Fragments arranged by size: 4299 1359 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2274 794 Size: 1480 5190 BstBI TT'CG_AA Cuts at: 3891 5474 3891 Size: 1583 5087 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstXI CCAn_nnnn'nTGG Cuts at: 2346 3514 2346 Size: 1168 5502 BstYI r'GATC_y Cuts at: 1972 3154 3225 4000 4367 4752 4769 6221 6307 Size: 1182 71 775 367 385 17 1452 86 Cuts at: 6307 6319 6665 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1182 775 385 367 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3831 3831 Size: 6670 BtgI C'CryG_G Cuts at: 513 1065 2004 2345 2949 3244 513 Size: 552 939 341 604 295 3939 Fragments arranged by size: 3939 939 604 552 341 295 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 487 Size: 683 56 812 5119 Fragments arranged by size: 5119 812 683 56 BtsI GCAGTG_nn' Cuts at: 2519 4186 4980 5000 2519 Size: 1667 794 20 4189 Fragments arranged by size: 4189 1667 794 20 ClaI AT'CG_AT Cuts at: 1554 3252 1554 Size: 1698 4972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2617 2652 529 Size: 35 2053 35 4547 Fragments arranged by size: 4547 2053 35 35 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2188 2348 3241 Size: 54 1425 50 275 18 358 160 893 Cuts at: 3241 4117 5006 6635 8 Size: 876 889 1629 43 Fragments arranged by size: 1629 1425 893 889 876 358 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4117 1537 Size: 275 2305 4090 Fragments arranged by size: 4090 2305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2782 3846 4600 1353 Size: 1429 1064 754 3423 Fragments arranged by size: 3423 1429 1064 754 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5257 5631 5777 387 Size: 1064 138 3668 374 146 1280 Fragments arranged by size: 3668 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2641 2873 2954 3206 3281 4733 5330 Size: 393 1009 232 81 252 75 1452 597 Cuts at: 5330 6117 6385 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1452 1009 787 597 393 268 252 232 81 75 EcoNI CCTnn'n_nnAGG Cuts at: 2293 2293 Size: 6670 EcoO109I rG'GnC_Cy Cuts at: 2010 2286 2912 2992 2993 3143 3144 3412 3413 Size: 276 626 80 1 150 1 268 1 Cuts at: 3413 2010 Size: 5267 Fragments arranged by size: 5267 626 276 268 150 80 1 1 1 EcoRI G'AATT_C Cuts at: 3598 3979 4357 3598 Size: 381 378 5911 Fragments arranged by size: 5911 381 378 EcoRV GAT'ATC Cuts at: 3256 3301 3256 Size: 45 6625 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3667 3667 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 3447 5817 1678 Size: 1769 2370 2531 Fragments arranged by size: 2531 2370 1769 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2992 3024 3677 3709 5311 Size: 32 135 32 1420 32 653 32 1602 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1602 1420 653 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2899 4014 4260 669 Size: 988 281 238 723 1115 246 3079 Fragments arranged by size: 3079 1115 988 723 281 246 238 HindIII A'AGCT_T Cuts at: 748 3263 748 Size: 2515 4155 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 2529 3995 2529 Size: 1466 5204 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2796 3227 3645 5784 5968 1305 Size: 1491 431 418 2139 184 2007 Fragments arranged by size: 2139 2007 1491 431 418 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2350 3243 10 Size: 54 1425 343 518 893 3437 Fragments arranged by size: 3437 1425 893 518 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2304 2344 4628 4987 5146 518 Size: 648 1138 40 2284 359 159 2042 Fragments arranged by size: 2284 2042 1138 648 359 159 40 MspA1I CmG'CkG Cuts at: 1606 3514 3681 3699 4086 4763 5911 6156 1606 Size: 1908 167 18 387 677 1148 245 2120 Fragments arranged by size: 2120 1908 1148 677 387 245 167 18 NcoI C'CATG_G Cuts at: 513 1065 2004 2345 2949 3244 513 Size: 552 939 341 604 295 3939 Fragments arranged by size: 3939 939 604 552 341 295 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 2416 3814 4028 5573 1132 Size: 97 1187 1398 214 1545 2229 Fragments arranged by size: 2229 1545 1398 1187 214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6670 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6670 PfoI T'CCnGG_A Cuts at: 2814 3933 2814 Size: 1119 5551 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 2286 2010 Size: 276 6394 PshAI GACnn'nnGTC Cuts at: 1613 2211 2331 1613 Size: 598 120 5952 Fragments arranged by size: 5952 598 120 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 2992 3143 3412 2992 Size: 151 269 6250 Fragments arranged by size: 6250 269 151 PspXI vC'TCGA_Gb Cuts at: 3124 3124 Size: 6670 PstI C_TGCA'G Cuts at: 830 2048 2270 3396 4022 830 Size: 1218 222 1126 626 3478 Fragments arranged by size: 3478 1218 1126 626 222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 3514 3514 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SexAI A'CCwGG_T Cuts at: 2592 2592 Size: 6670 SfcI C'TryA_G Cuts at: 826 1045 2044 2266 2329 3392 4018 5153 5834 Size: 219 999 222 63 1063 626 1135 681 Cuts at: 5834 6025 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1063 999 681 626 222 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3997 1872 Size: 2125 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3124 3593 4774 5675 5937 820 Size: 197 926 1181 469 1181 901 262 1553 Fragments arranged by size: 1553 1181 1181 926 901 469 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 2144 1517 Size: 627 6043 StyI C'CwwG_G Cuts at: 513 1065 2004 2345 2616 2949 3244 4109 513 Size: 552 939 341 271 333 295 865 3074 Fragments arranged by size: 3074 939 865 552 341 333 295 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2403 3524 4697 4714 4867 5052 5497 Size: 1255 823 1121 1173 17 153 185 445 Cuts at: 5497 325 Size: 1498 Fragments arranged by size: 1498 1255 1173 1121 823 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4916 6628 96 Size: 274 80 33 51 1443 2939 1712 138 Fragments arranged by size: 2939 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 2728 2760 6630 6662 2489 Size: 32 207 32 3870 32 2497 Fragments arranged by size: 3870 2497 207 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2630 1316 Size: 1314 5356 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3166 3166 Size: 6670 XhoI C'TCGA_G Cuts at: 1943 3124 1943 Size: 1181 5489 XmaI C'CCGG_G Cuts at: 1870 3995 1870 Size: 2125 4545 XmnI GAAnn'nnTTC Cuts at: 3855 4799 3855 Size: 944 5726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI