(Circular) (Six-base) MAPSORT of: pFN21AE3587.seq Check: 4816 from: 1 to: 5419 pFN21AE3587 5419 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2142 2149 2306 2676 2142 Size: 7 157 370 4885 Fragments arranged by size: 4885 370 157 7 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3229 278 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2762 1937 Size: 825 4594 Acc65I G'GTAC_C Cuts at: 2740 2740 Size: 5419 AclI AA'CG_TT Cuts at: 1563 3546 3919 1563 Size: 1983 373 3063 Fragments arranged by size: 3063 1983 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 4866 5134 1632 Size: 1850 1384 268 1917 Fragments arranged by size: 1917 1850 1384 268 AfeI AGC'GCT Cuts at: 2324 2387 2324 Size: 63 5356 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5222 AflIII A'CryG_T Cuts at: 1203 4318 5295 1203 Size: 3115 977 1327 Fragments arranged by size: 3115 1327 977 AgeI A'CCGG_T Cuts at: 4250 4250 Size: 5419 AhdI GACnn_n'nnGTC Cuts at: 4148 4148 Size: 5419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5419 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5419 AlwNI CAG_nnn'CTG Cuts at: 2134 3162 4734 2134 Size: 1028 1572 2819 Fragments arranged by size: 2819 1572 1028 ApaI G_GGCC'C Cuts at: 2079 2222 2241 2567 2079 Size: 143 19 326 4931 Fragments arranged by size: 4931 326 143 19 ApaLI G'TGCA_C Cuts at: 3476 4632 3476 Size: 1156 4263 ApoI r'AATT_y Cuts at: 1493 2728 2954 3106 5215 1493 Size: 1235 226 152 2109 1697 Fragments arranged by size: 2109 1697 1235 226 152 AseI AT'TA_AT Cuts at: 160 1032 3973 160 Size: 872 2941 1606 Fragments arranged by size: 2941 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 AvaI C'yCGr_G Cuts at: 1870 1943 2209 2222 2744 1870 Size: 73 266 13 522 4545 Fragments arranged by size: 4545 522 266 73 13 BamHI G'GATC_C Cuts at: 2749 3116 2749 Size: 367 5052 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2617 2740 4195 618 Size: 325 217 627 830 123 1455 1842 Fragments arranged by size: 1842 1455 830 627 325 217 123 BanII G_rGCy'C Cuts at: 1391 2079 2222 2241 2567 2738 1391 Size: 688 143 19 326 171 4072 Fragments arranged by size: 4072 688 326 171 143 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2598 928 Size: 971 699 3749 Fragments arranged by size: 3749 971 699 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3610 3644 1903 Size: 34 1673 34 3678 Fragments arranged by size: 3678 1673 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3311 4521 3311 Size: 1210 4209 BclI T'GATC_A Cuts at: 1548 1548 Size: 5419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2286 4030 Size: 107 122 71 631 605 337 277 1744 Cuts at: 4030 136 Size: 1525 Fragments arranged by size: 1744 1525 631 605 337 277 122 107 71 BglII A'GATC_T Cuts at: 5414 5414 Size: 5419 BlpI GC'TnA_GC Cuts at: 2836 2836 Size: 5419 Bme1580I G_kGCm'C Cuts at: 1790 2046 2079 2222 2241 2567 2622 3480 4636 Size: 256 33 143 19 326 55 858 1156 Cuts at: 4636 1790 Size: 2573 Fragments arranged by size: 2573 1156 858 326 256 143 55 33 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 3150 4108 Size: 416 53 83 399 24 1128 605 958 Cuts at: 4108 442 Size: 1753 Fragments arranged by size: 1753 1128 958 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4079 1239 Size: 2840 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 3544 4409 4707 2648 Size: 896 865 298 3360 Fragments arranged by size: 3360 896 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4082 882 Size: 617 2583 2219 Fragments arranged by size: 2583 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 3210 493 Size: 711 1059 947 2702 Fragments arranged by size: 2702 1059 947 711 BsaBI GATnn'nnATC Cuts at: 5413 5413 Size: 5419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3226 3608 4243 275 Size: 53 83 186 936 1693 382 635 1451 Fragments arranged by size: 1693 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3852 4250 4524 4671 5082 3852 Size: 398 274 147 411 4189 Fragments arranged by size: 4189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 4138 Fragments arranged by size: 4138 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5419 BseYI C'CCAG_C Cuts at: 2089 2325 4622 5118 2089 Size: 236 2297 496 2390 Fragments arranged by size: 2390 2297 496 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 2450 1163 Size: 573 441 273 4132 Fragments arranged by size: 4132 573 441 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2342 2869 3630 4658 1540 Size: 275 186 341 527 761 1028 2301 Fragments arranged by size: 2301 1028 761 527 341 275 186 BsiHKAI G_wGCw'C Cuts at: 3480 3565 4636 3480 Size: 85 1071 4263 Fragments arranged by size: 4263 1071 85 BsmI GAATG_Cn' Cuts at: 2930 3023 2930 Size: 93 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2079 2222 2241 2567 2622 2738 Size: 399 256 33 143 19 326 55 116 Cuts at: 2738 3480 3565 4636 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 326 256 143 116 85 55 33 19 BspEI T'CCGG_A Cuts at: 5082 5082 Size: 5419 BspHI T'CATG_A Cuts at: 2700 3308 5038 2700 Size: 608 1730 3081 Fragments arranged by size: 3081 1730 608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 2149 2306 2472 2676 2774 844 Size: 1298 7 157 166 204 98 3489 Fragments arranged by size: 3489 1298 204 166 157 98 7 BsrBI CCG'CTC Cuts at: 1394 1823 3306 1394 Size: 429 1483 3507 Fragments arranged by size: 3507 1483 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3914 4088 65 Size: 1183 2666 174 1396 Fragments arranged by size: 2666 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 2337 2423 2533 4063 4250 1700 Size: 110 287 240 86 110 1530 187 2869 Fragments arranged by size: 2869 1530 287 240 187 110 110 86 BsrGI T'GTAC_A Cuts at: 96 2268 96 Size: 2172 3247 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5419 BssSI C'ACGA_G Cuts at: 2039 2429 3479 4491 2039 Size: 390 1050 1012 2967 Fragments arranged by size: 2967 1050 1012 390 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 794 Size: 1469 3950 BstBI TT'CG_AA Cuts at: 4223 4223 Size: 5419 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5419 BstYI r'GATC_y Cuts at: 1972 2749 3116 3501 3518 4970 5056 5068 5414 Size: 777 367 385 17 1452 86 12 346 Cuts at: 5414 1972 Size: 1977 Fragments arranged by size: 1977 1452 777 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2613 2613 Size: 5419 BtgI C'CryG_G Cuts at: 513 1065 2004 2559 2679 513 Size: 552 939 555 120 3253 Fragments arranged by size: 3253 939 555 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2499 2671 487 Size: 683 56 1273 172 3235 Fragments arranged by size: 3235 1273 683 172 56 BtsI GCAGTG_nn' Cuts at: 2935 3729 3749 2935 Size: 794 20 4605 Fragments arranged by size: 4605 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5384 DraI TTT'AAA Cuts at: 2723 2723 Size: 5419 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4426 809 Size: 507 3110 1802 Fragments arranged by size: 3110 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2339 2425 2676 Size: 54 1425 50 275 18 509 86 251 Cuts at: 2676 2866 3755 5384 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1425 889 509 275 251 190 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2339 2866 1537 Size: 275 527 527 4090 Fragments arranged by size: 4090 527 527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 3349 1353 Size: 1274 722 3423 Fragments arranged by size: 3423 1274 722 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4006 4380 4526 387 Size: 1064 138 2417 374 146 1280 Fragments arranged by size: 2417 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3482 4079 4866 5134 1239 Size: 393 1850 597 787 268 1524 Fragments arranged by size: 1850 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2075 2219 2237 2564 2075 Size: 144 18 327 4930 Fragments arranged by size: 4930 327 144 18 EcoRI G'AATT_C Cuts at: 2728 3106 2728 Size: 378 5041 FspI TGC'GCA Cuts at: 3925 3925 Size: 5419 HaeII r_GCGC'y Cuts at: 1678 2090 2326 2389 2397 4566 1678 Size: 412 236 63 8 2169 2531 Fragments arranged by size: 2531 2169 412 236 63 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373 Size: 32 135 32 2488 32 42 32 2626 Fragments arranged by size: 2626 2488 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2763 3009 669 Size: 988 281 825 246 3079 Fragments arranged by size: 3079 988 825 281 246 HindIII A'AGCT_T Cuts at: 748 2624 748 Size: 1876 3543 HpaI GTT'AAC Cuts at: 3009 3009 Size: 5419 KpnI G_GTAC'C Cuts at: 2744 2744 Size: 5419 MfeI C'AATT_G Cuts at: 3018 3018 Size: 5419 MluI A'CGCG_T Cuts at: 5295 5295 Size: 5419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 4533 4717 1305 Size: 843 2385 184 2007 Fragments arranged by size: 2385 2007 843 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3597 Fragments arranged by size: 3597 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3377 3736 3895 518 Size: 648 2211 359 159 2042 Fragments arranged by size: 2211 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2053 2062 2643 2835 3512 4660 4905 Size: 414 33 9 581 192 677 1148 245 Cuts at: 4905 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 581 414 245 192 33 9 NaeI GCC'GGC Cuts at: 2425 2425 Size: 5419 NcoI C'CATG_G Cuts at: 513 1065 2004 2559 513 Size: 552 939 555 3373 Fragments arranged by size: 3373 939 555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5419 NgoMIV G'CCGG_C Cuts at: 2423 2423 Size: 5419 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5419 NotI GC'GGCC_GC Cuts at: 2866 2866 Size: 5419 NspI r_CATG'y Cuts at: 1132 1229 2105 2777 4322 1132 Size: 97 876 672 1545 2229 Fragments arranged by size: 2229 1545 876 672 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5419 PciI A'CATG_T Cuts at: 4318 4318 Size: 5419 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5419 PmeI GTTT'AAAC Cuts at: 2723 2723 Size: 5419 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3488 3520 5001 5033 3488 Size: 32 1481 32 3874 Fragments arranged by size: 3874 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5419 PsiI TTA'TAA Cuts at: 2989 2989 Size: 5419 PspOMI G'GGCC_C Cuts at: 2075 2218 2237 2563 2075 Size: 143 19 326 4931 Fragments arranged by size: 4931 326 143 19 PstI C_TGCA'G Cuts at: 830 2771 830 Size: 1941 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5419 SalI G'TCGA_C Cuts at: 1936 2761 1936 Size: 825 4594 SapI GCTCTTCn'nnn_ Cuts at: 2627 2627 Size: 5419 SbfI CC_TGCA'GG Cuts at: 2771 2771 Size: 5419 ScaI AGT'ACT Cuts at: 2435 3667 5379 2435 Size: 1232 1712 2475 Fragments arranged by size: 2475 1712 1232 SfcI C'TryA_G Cuts at: 826 1045 2389 2767 3902 4583 4774 826 Size: 219 1344 378 1135 681 191 1471 Fragments arranged by size: 1471 1344 1135 681 378 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5419 SmaI CCC'GGG Cuts at: 1872 2224 2746 1872 Size: 352 522 4545 Fragments arranged by size: 4545 522 352 SmlI C'TyrA_G Cuts at: 820 1017 1943 2627 3523 4424 4686 820 Size: 197 926 684 896 901 262 1553 Fragments arranged by size: 1553 926 901 896 684 262 197 SnaBI TAC'GTA Cuts at: 493 3210 493 Size: 2717 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5419 SphI G_CATG'C Cuts at: 1132 2105 2777 1132 Size: 973 672 3774 Fragments arranged by size: 3774 973 672 SspI AAT'ATT Cuts at: 5 52 3343 5 Size: 47 3291 2081 Fragments arranged by size: 3291 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5419 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 2559 2858 513 Size: 552 939 67 488 299 3074 Fragments arranged by size: 3074 939 552 488 299 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3446 3463 3616 3801 4246 325 Size: 1255 1866 17 153 185 445 1498 Fragments arranged by size: 1866 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2268 2433 Size: 274 80 33 51 1443 132 159 165 Cuts at: 2433 3665 5377 96 Size: 1232 1712 138 Fragments arranged by size: 1712 1443 1232 274 165 159 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5379 5411 5379 Size: 32 5387 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5419 XbaI T'CTAG_A Cuts at: 2755 2755 Size: 5419 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5419 XmaI C'CCGG_G Cuts at: 1870 2222 2744 1870 Size: 352 522 4545 Fragments arranged by size: 4545 522 352 XmnI GAAnn'nnTTC Cuts at: 2492 3548 2492 Size: 1056 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3227 276 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI