(Circular) (Six-base) MAPSORT of: pFN21AB7865.seq Check: 3360 from: 1 to: 6376 pFN21AB7865 6376 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2892 3719 1937 Size: 955 827 4594 Fragments arranged by size: 4594 955 827 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4439 5823 6091 1632 Size: 2807 1384 268 1917 Fragments arranged by size: 2807 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 3537 3639 5275 6252 1203 Size: 2334 102 1636 977 1327 Fragments arranged by size: 2334 1636 1327 977 102 AgeI A'CCGG_T Cuts at: 2706 5207 2706 Size: 2501 3875 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 2611 3436 4119 5691 2611 Size: 825 683 1572 3296 Fragments arranged by size: 3296 1572 825 683 ApaI G_GGCC'C Cuts at: 2724 2724 Size: 6376 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3701 1870 Size: 73 1758 4545 Fragments arranged by size: 4545 1758 73 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 3697 5152 618 Size: 325 217 627 687 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 687 627 325 217 BanII G_rGCy'C Cuts at: 1391 2060 2172 2243 2682 2724 2867 3476 3695 Size: 669 112 71 439 42 143 609 219 Cuts at: 3695 1391 Size: 4072 Fragments arranged by size: 4072 669 609 439 219 143 112 71 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2179 2492 3636 928 Size: 971 280 313 1144 3668 Fragments arranged by size: 3668 1144 971 313 280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 2376 1548 Size: 828 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 2054 3793 2054 Size: 1739 4637 Bme1580I G_kGCm'C Cuts at: 1790 2477 2724 4437 5593 1790 Size: 687 247 1713 1156 2573 Fragments arranged by size: 2573 1713 1156 687 247 BmgBI CAC'GTC Cuts at: 1318 2022 1318 Size: 704 5672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3233 3559 4107 Size: 416 53 83 399 24 1816 326 548 Cuts at: 4107 5065 442 Size: 958 1753 Fragments arranged by size: 1816 1753 958 548 416 399 326 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2012 2153 3342 5036 1239 Size: 773 141 1189 1694 2579 Fragments arranged by size: 2579 1694 1189 773 141 Bpu10I CC'TnA_GC Cuts at: 2042 2042 Size: 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 4501 5366 5664 2264 Size: 2237 865 298 2976 Fragments arranged by size: 2976 2237 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2952 4183 4565 5200 Size: 53 83 186 936 1419 1231 382 635 Cuts at: 5200 275 Size: 1451 Fragments arranged by size: 1451 1419 1231 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 2706 4809 5207 5481 5628 6039 2337 Size: 369 2103 398 274 147 411 2674 Fragments arranged by size: 2674 2103 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 3254 3284 1178 Size: 30 165 30 822 30 999 30 4270 Fragments arranged by size: 4270 999 822 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2321 2936 3016 3021 3082 3179 3240 Size: 925 210 615 80 5 61 97 61 Cuts at: 3240 1186 Size: 4322 Fragments arranged by size: 4322 925 615 210 97 80 61 61 5 BseYI C'CCAG_C Cuts at: 2457 2730 2771 3020 3160 3285 3429 3492 5579 Size: 273 41 249 140 125 144 63 2087 Cuts at: 5579 6075 2457 Size: 496 2758 Fragments arranged by size: 2758 2087 496 273 249 144 140 125 63 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3422 1163 Size: 573 1686 4117 Fragments arranged by size: 4117 1686 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 3826 4587 5615 1540 Size: 275 186 757 1068 761 1028 2301 Fragments arranged by size: 2301 1068 1028 761 757 275 186 BsiHKAI G_wGCw'C Cuts at: 2060 4437 4522 5593 2060 Size: 2377 85 1071 2843 Fragments arranged by size: 2843 2377 1071 85 BsmI GAATG_Cn' Cuts at: 2810 2927 3887 3980 2810 Size: 117 960 93 5206 Fragments arranged by size: 5206 960 117 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 2129 Size: 6376 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2172 2243 2477 2682 2724 2867 Size: 399 270 112 71 234 205 42 143 Cuts at: 2867 3476 3695 4437 4522 5593 1391 Size: 609 219 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 399 270 234 219 205 143 112 85 71 42 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 3306 4265 5995 3306 Size: 959 1730 3687 Fragments arranged by size: 3687 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3731 844 Size: 2887 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2504 4263 1394 Size: 429 681 1759 3507 Fragments arranged by size: 3507 1759 681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3603 4871 5045 65 Size: 1183 2355 1268 174 1396 Fragments arranged by size: 2355 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 2432 2706 3392 5020 5207 1700 Size: 110 372 250 274 686 1628 187 2869 Fragments arranged by size: 2869 1628 686 372 274 250 187 110 BsrGI T'GTAC_A Cuts at: 96 2508 96 Size: 2412 3964 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6376 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 2065 3127 1184 Size: 881 1062 4433 Fragments arranged by size: 4433 1062 881 BstXI CCAn_nnnn'nTGG Cuts at: 2573 2651 2573 Size: 78 6298 BstYI r'GATC_y Cuts at: 1972 2538 3706 4073 4458 4475 5927 6013 6025 Size: 566 1168 367 385 17 1452 86 12 Cuts at: 6025 6371 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1168 566 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2644 2854 2946 2998 3109 3123 Size: 552 939 640 210 92 52 111 14 Cuts at: 3123 513 Size: 3766 Fragments arranged by size: 3766 939 640 552 210 111 92 52 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 3892 4686 4706 3892 Size: 794 20 5562 Fragments arranged by size: 5562 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DraIII CAC_nnn'GTG Cuts at: 3368 3368 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3136 5383 809 Size: 507 1820 2247 1802 Fragments arranged by size: 2247 1820 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2943 3390 Size: 54 1425 50 275 18 376 737 447 Cuts at: 3390 3823 4712 6341 8 Size: 433 889 1629 43 Fragments arranged by size: 1629 1425 889 737 447 433 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2775 4306 1353 Size: 1422 1531 3423 Fragments arranged by size: 3423 1531 1422 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 2342 4963 5337 5483 387 Size: 1064 138 741 12 2621 374 146 1280 Fragments arranged by size: 2621 1280 1064 741 374 146 138 12 EcoICRI GAG'CTC Cuts at: 2058 2058 Size: 6376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2153 3342 4439 5036 5823 6091 Size: 393 380 141 1189 1097 597 787 268 Cuts at: 6091 1239 Size: 1524 Fragments arranged by size: 1524 1189 1097 787 597 393 380 268 141 EcoO109I rG'GnC_Cy Cuts at: 2353 2720 2353 Size: 367 6009 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 2239 2203 Size: 36 6340 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2049 2297 3123 5523 1678 Size: 371 248 826 2400 2531 Fragments arranged by size: 2531 2400 826 371 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2657 2689 5017 Size: 32 135 32 653 32 400 32 2328 Cuts at: 5017 5049 5091 5123 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2328 653 400 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2636 3720 3966 669 Size: 988 281 698 1084 246 3079 Fragments arranged by size: 3079 1084 988 698 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3333 3975 3333 Size: 642 5734 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 2892 2988 5490 5674 1305 Size: 1264 323 96 2502 184 2007 Fragments arranged by size: 2502 2007 1264 323 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2945 10 Size: 54 1425 343 1113 3441 Fragments arranged by size: 3441 1425 1113 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 3638 4334 4693 4852 518 Size: 648 1314 1158 696 359 159 2042 Fragments arranged by size: 2042 1314 1158 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2323 2608 3436 3512 3523 3792 4469 Size: 554 163 285 828 76 11 269 677 Cuts at: 4469 5617 5862 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 828 677 554 285 269 245 163 76 11 NaeI GCC'GGC Cuts at: 3394 3394 Size: 6376 NcoI C'CATG_G Cuts at: 513 1065 2004 2644 2946 3109 3123 513 Size: 552 939 640 302 163 14 3766 Fragments arranged by size: 3766 939 640 552 302 163 14 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NgoMIV G'CCGG_C Cuts at: 3392 3392 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 2392 3541 3643 3734 5279 1132 Size: 97 1163 1149 102 91 1545 2229 Fragments arranged by size: 2229 1545 1163 1149 102 97 91 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 3537 3639 5275 3537 Size: 102 1636 4638 Fragments arranged by size: 4638 1636 102 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 1108 Size: 1752 4624 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2353 2353 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 2720 2720 Size: 6376 PstI C_TGCA'G Cuts at: 830 2292 3414 3528 3728 830 Size: 1462 1122 114 200 3478 Fragments arranged by size: 3478 1462 1122 200 114 PvuI CG_AT'CG Cuts at: 2001 2758 2001 Size: 757 5619 PvuII CAG'CTG Cuts at: 2608 3436 3512 3523 2608 Size: 828 76 11 5461 Fragments arranged by size: 5461 828 76 11 SacI G_AGCT'C Cuts at: 2060 2060 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SexAI A'CCwGG_T Cuts at: 2513 2513 Size: 6376 SfcI C'TryA_G Cuts at: 826 1045 2288 2956 3058 3410 3524 3724 4859 Size: 219 1243 668 102 352 114 200 1135 Cuts at: 4859 5540 5731 826 Size: 681 191 1471 Fragments arranged by size: 1471 1243 1135 681 668 352 219 200 191 114 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 4480 5381 5643 820 Size: 197 926 336 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 2090 2750 2792 1517 Size: 573 660 42 5101 Fragments arranged by size: 5101 660 573 42 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2091 2214 2357 2644 2946 Size: 552 939 66 21 123 143 287 302 Cuts at: 2946 3109 3123 3815 513 Size: 163 14 692 3074 Fragments arranged by size: 3074 939 692 552 302 287 163 143 123 66 21 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4403 4420 4573 4758 5203 325 Size: 1255 2823 17 153 185 445 1498 Fragments arranged by size: 2823 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 3626 4622 Size: 274 80 33 51 1443 531 1118 996 Cuts at: 4622 6334 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1118 996 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 2332 2364 3254 3286 3354 3386 6336 Size: 32 41 32 890 32 68 32 2950 Cuts at: 6336 6368 2259 Size: 32 2267 Fragments arranged by size: 2950 2267 890 68 41 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2077 2077 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI