Restriction map for
pFN21AB7865
(Linear) (Six-base) MAPSORT of: KIBB7865.seq Check: 8490 from: 1 to: 1671
KIBB7865.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 887 1671
Size: 887 784
AflIII A'CryG_T
Cuts at: 0 1532 1634 1671
Size: 1532 102 37
Fragments arranged by size:
1532 102 37
AgeI A'CCGG_T
Cuts at: 0 701 1671
Size: 701 970
AlwNI CAG_nnn'CTG
Cuts at: 0 606 1431 1671
Size: 606 825 240
Fragments arranged by size:
825 606 240
ApaI G_GGCC'C
Cuts at: 0 719 1671
Size: 719 952
BanI G'GyrC_C
Cuts at: 0 469 1671
Size: 469 1202
BanII G_rGCy'C
Cuts at: 0 55 167 238 677 719 862 1471 1671
Size: 55 112 71 439 42 143 609 200
Fragments arranged by size:
609 439 200 143 112 71 55 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 174 487 1631 1671
Size: 174 313 1144 40
Fragments arranged by size:
1144 313 174 40
BclI T'GATC_A
Cuts at: 0 371 1671
Size: 371 1300
BlpI GC'TnA_GC
Cuts at: 0 49 1671
Size: 49 1622
Bme1580I G_kGCm'C
Cuts at: 0 472 719 1671
Size: 472 247 952
Fragments arranged by size:
952 472 247
BmgBI CAC'GTC
Cuts at: 0 17 1671
Size: 17 1654
BmrI ACTGGGnnnn_n'
Cuts at: 0 1228 1554 1671
Size: 1228 326 117
Fragments arranged by size:
1228 326 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 1671
Size: 553 1118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 148 1337 1671
Size: 7 141 1189 334
Fragments arranged by size:
1189 334 141 7
Bpu10I CC'TnA_GC
Cuts at: 0 37 1671
Size: 37 1634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1671
Size: 259 1412
BsaHI Gr'CG_yC
Cuts at: 0 947 1671
Size: 947 724
BsaWI w'CCGG_w
Cuts at: 0 332 701 1671
Size: 332 369 970
Fragments arranged by size:
970 369 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 1249 1279 1671
Size: 220 30 999 30 392
Fragments arranged by size:
999 392 220 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 316 931 1011 1016 1077 1174 1235
Size: 106 210 615 80 5 61 97 61
Cuts at: 1235 1671
Size: 436
Fragments arranged by size:
615 436 210 106 97 80 61 61
5
BseYI C'CCAG_C
Cuts at: 0 452 725 766 1015 1155 1280 1424 1487
Size: 452 273 41 249 140 125 144 63
Cuts at: 1487 1671
Size: 184
Fragments arranged by size:
452 273 249 184 144 140 125 63
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1417 1671
Size: 1417 254
BsiEI CG_ry'CG
Cuts at: 0 753 1671
Size: 753 918
BsiHKAI G_wGCw'C
Cuts at: 0 55 1671
Size: 55 1616
BsmI GAATG_Cn'
Cuts at: 0 805 922 1671
Size: 805 117 749
Fragments arranged by size:
805 749 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 1671
Size: 124 1547
Bsp1286I G_dGCh'C
Cuts at: 0 55 167 238 472 677 719 862 1471
Size: 55 112 71 234 205 42 143 609
Cuts at: 1471 1671
Size: 200
Fragments arranged by size:
609 234 205 200 143 112 71 55
42
BspHI T'CATG_A
Cuts at: 0 1301 1671
Size: 1301 370
BsrBI CCG'CTC
Cuts at: 0 499 1671
Size: 499 1172
BsrDI GCAATG_nn'
Cuts at: 0 1598 1671
Size: 1598 73
BsrFI r'CCGG_y
Cuts at: 0 177 427 701 1387 1671
Size: 177 250 274 686 284
Fragments arranged by size:
686 284 274 250 177
BsrGI T'GTAC_A
Cuts at: 0 503 1671
Size: 503 1168
BstEII G'GTnAC_C
Cuts at: 0 60 1122 1671
Size: 60 1062 549
Fragments arranged by size:
1062 549 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 568 646 1671
Size: 568 78 1025
Fragments arranged by size:
1025 568 78
BstYI r'GATC_y
Cuts at: 0 533 1671
Size: 533 1138
BtgI C'CryG_G
Cuts at: 0 639 849 941 993 1104 1118 1671
Size: 639 210 92 52 111 14 553
Fragments arranged by size:
639 553 210 111 92 52 14
DraIII CAC_nnn'GTG
Cuts at: 0 1363 1671
Size: 1363 308
DrdI GACnn_nn'nnGTC
Cuts at: 0 1131 1671
Size: 1131 540
EaeI y'GGCC_r
Cuts at: 0 201 938 1385 1671
Size: 201 737 447 286
Fragments arranged by size:
737 447 286 201
EarI CTCTTCn'nnn_
Cuts at: 0 770 1671
Size: 770 901
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 337 1671
Size: 325 12 1334
Fragments arranged by size:
1334 325 12
EcoICRI GAG'CTC
Cuts at: 0 53 1671
Size: 53 1618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 148 1337 1671
Size: 7 141 1189 334
Fragments arranged by size:
1189 334 141 7
EcoO109I rG'GnC_Cy
Cuts at: 0 348 715 1671
Size: 348 367 956
Fragments arranged by size:
956 367 348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 234 1671
Size: 198 36 1437
Fragments arranged by size:
1437 198 36
HaeII r_GCGC'y
Cuts at: 0 44 292 1118 1671
Size: 44 248 826 553
Fragments arranged by size:
826 553 248 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 652 684 1671
Size: 220 32 400 32 987
Fragments arranged by size:
987 400 220 32 32
HincII GTy'rAC
Cuts at: 0 631 1671
Size: 631 1040
MfeI C'AATT_G
Cuts at: 0 1328 1671
Size: 1328 343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 887 983 1671
Size: 564 323 96 688
Fragments arranged by size:
688 564 323 96
MscI TGG'CCA
Cuts at: 0 940 1671
Size: 940 731
MslI CAynn'nnrTG
Cuts at: 0 475 1633 1671
Size: 475 1158 38
Fragments arranged by size:
1158 475 38
MspA1I CmG'CkG
Cuts at: 0 155 318 603 1431 1507 1518 1671
Size: 155 163 285 828 76 11 153
Fragments arranged by size:
828 285 163 155 153 76 11
NaeI GCC'GGC
Cuts at: 0 1389 1671
Size: 1389 282
NcoI C'CATG_G
Cuts at: 0 639 941 1104 1118 1671
Size: 639 302 163 14 553
Fragments arranged by size:
639 553 302 163 14
NgoMIV G'CCGG_C
Cuts at: 0 1387 1671
Size: 1387 284
NspI r_CATG'y
Cuts at: 0 387 1536 1638 1671
Size: 387 1149 102 33
Fragments arranged by size:
1149 387 102 33
PciI A'CATG_T
Cuts at: 0 1532 1634 1671
Size: 1532 102 37
Fragments arranged by size:
1532 102 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 1671
Size: 855 816
PpuMI rG'GwC_Cy
Cuts at: 0 348 1671
Size: 348 1323
PspOMI G'GGCC_C
Cuts at: 0 715 1671
Size: 715 956
PstI C_TGCA'G
Cuts at: 0 287 1409 1523 1671
Size: 287 1122 114 148
Fragments arranged by size:
1122 287 148 114
PvuI CG_AT'CG
Cuts at: 0 753 1671
Size: 753 918
PvuII CAG'CTG
Cuts at: 0 603 1431 1507 1518 1671
Size: 603 828 76 11 153
Fragments arranged by size:
828 603 153 76 11
SacI G_AGCT'C
Cuts at: 0 55 1671
Size: 55 1616
SexAI A'CCwGG_T
Cuts at: 0 508 1671
Size: 508 1163
SfcI C'TryA_G
Cuts at: 0 283 951 1053 1405 1519 1671
Size: 283 668 102 352 114 152
Fragments arranged by size:
668 352 283 152 114 102
SmlI C'TyrA_G
Cuts at: 0 274 1671
Size: 274 1397
StuI AGG'CCT
Cuts at: 0 85 745 787 1671
Size: 85 660 42 884
Fragments arranged by size:
884 660 85 42
StyI C'CwwG_G
Cuts at: 0 65 86 209 352 639 941 1104 1118
Size: 65 21 123 143 287 302 163 14
Cuts at: 1118 1671
Size: 553
Fragments arranged by size:
553 302 287 163 143 123 65 21
14
TatI w'GTAC_w
Cuts at: 0 503 1621 1671
Size: 503 1118 50
Fragments arranged by size:
1118 503 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 327 359 1249 1281 1349 1381
Size: 254 32 41 32 890 32 68 32
Cuts at: 1381 1671
Size: 290
Fragments arranged by size:
890 290 254 68 41 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 1671
Size: 72 1599
Enzymes that do cut:
AccI AflIII AgeI AlwNI ApaI BanI BanII BbsI
BclI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI
BsrGI BstEII BstXI BstYI BtgI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PciI PflMI PpuMI PspOMI PstI PvuI
PvuII SacI SexAI SfcI SmlI StuI StyI TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BmtI BsaI BsaAI BsaBI BsiWI
BspEI BspMI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EagI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI