(Circular) (Six-base) MAPSORT of: pFN21AE1547.seq Check: 4890 from: 1 to: 6478 pFN21AE1547 6478 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4288 278 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3821 1937 Size: 1884 4594 Acc65I G'GTAC_C Cuts at: 3799 3799 Size: 6478 AclI AA'CG_TT Cuts at: 1563 4605 4978 1563 Size: 3042 373 3063 Fragments arranged by size: 3063 3042 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2663 4541 5925 6193 1632 Size: 1031 1878 1384 268 1917 Fragments arranged by size: 1917 1878 1384 1031 268 AflII C'TTAA_G Cuts at: 820 1017 3221 820 Size: 197 2204 4077 Fragments arranged by size: 4077 2204 197 AflIII A'CryG_T Cuts at: 1203 3126 5377 6354 1203 Size: 1923 2251 977 1327 Fragments arranged by size: 2251 1923 1327 977 AgeI A'CCGG_T Cuts at: 5309 5309 Size: 6478 AhdI GACnn_n'nnGTC Cuts at: 5207 5207 Size: 6478 AleI CACnn'nnGTG Cuts at: 1166 3589 3683 1166 Size: 2423 94 3961 Fragments arranged by size: 3961 2423 94 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6478 AlwNI CAG_nnn'CTG Cuts at: 4221 5793 4221 Size: 1572 4906 ApaI G_GGCC'C Cuts at: 3262 3262 Size: 6478 ApaLI G'TGCA_C Cuts at: 3582 4535 5691 3582 Size: 953 1156 4369 Fragments arranged by size: 4369 1156 953 ApoI r'AATT_y Cuts at: 1493 3787 4013 4165 6274 1493 Size: 2294 226 152 2109 1697 Fragments arranged by size: 2294 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5032 160 Size: 872 4000 1606 Fragments arranged by size: 4000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2478 3200 3612 3803 1870 Size: 73 172 363 722 412 191 4545 Fragments arranged by size: 4545 722 412 363 191 172 73 BamHI G'GATC_C Cuts at: 3808 4175 3808 Size: 367 6111 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3083 3685 3799 5254 Size: 325 217 627 248 1048 602 114 1455 Cuts at: 5254 618 Size: 1842 Fragments arranged by size: 1842 1455 1048 627 602 325 248 217 114 BanII G_rGCy'C Cuts at: 1391 2460 2774 3058 3262 3797 1391 Size: 1069 314 284 204 535 4072 Fragments arranged by size: 4072 1069 535 314 284 204 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 6478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3756 928 Size: 971 1857 3650 Fragments arranged by size: 3650 1857 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3671 3705 4669 4703 1903 Size: 34 1734 34 964 34 3678 Fragments arranged by size: 3678 1734 964 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4370 5580 4370 Size: 1210 5268 BclI T'GATC_A Cuts at: 1548 2622 1548 Size: 1074 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5089 136 Size: 107 122 71 631 605 3417 1525 Fragments arranged by size: 3417 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6473 6473 Size: 6478 BlpI GC'TnA_GC Cuts at: 2459 3895 2459 Size: 1436 5042 Bme1580I G_kGCm'C Cuts at: 1790 3262 3586 4539 5695 1790 Size: 1472 324 953 1156 2573 Fragments arranged by size: 2573 1472 1156 953 324 BmgBI CAC'GTC Cuts at: 1318 3129 1318 Size: 1811 4667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3442 3532 4209 Size: 416 53 83 399 24 2025 90 677 Cuts at: 4209 5167 442 Size: 958 1753 Fragments arranged by size: 2025 1753 958 677 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6478 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2474 Size: 6478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2382 5138 1239 Size: 1143 2756 2579 Fragments arranged by size: 2756 2579 1143 Bpu10I CC'TnA_GC Cuts at: 2398 2852 2398 Size: 454 6024 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4603 5468 5766 4603 Size: 865 298 5315 Fragments arranged by size: 5315 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5141 882 Size: 617 3642 2219 Fragments arranged by size: 3642 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4269 493 Size: 711 3065 2702 Fragments arranged by size: 3065 2702 711 BsaBI GATnn'nnATC Cuts at: 2970 6472 2970 Size: 3502 2976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 4285 4667 5302 Size: 53 83 186 936 503 2249 382 635 Cuts at: 5302 275 Size: 1451 Fragments arranged by size: 2249 1451 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4911 5309 5583 5730 6141 4911 Size: 398 274 147 411 5248 Fragments arranged by size: 5248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2879 2909 1178 Size: 30 165 30 1476 30 4747 Fragments arranged by size: 4747 1476 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2735 1186 Size: 1549 4929 BseYI C'CCAG_C Cuts at: 5681 6177 5681 Size: 496 5982 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5905 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3928 4689 5717 1540 Size: 275 186 1927 761 1028 2301 Fragments arranged by size: 2301 1927 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2460 2774 3586 4539 4624 5695 2460 Size: 314 812 953 85 1071 3243 Fragments arranged by size: 3243 1071 953 812 314 85 BsmI GAATG_Cn' Cuts at: 3989 4082 3989 Size: 93 6385 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2460 2774 3058 3262 3586 3797 4539 Size: 399 670 314 284 204 324 211 742 Cuts at: 4539 4624 5695 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 399 324 314 284 211 204 85 BspEI T'CCGG_A Cuts at: 6141 6141 Size: 6478 BspHI T'CATG_A Cuts at: 3415 4367 6097 3415 Size: 952 1730 3796 Fragments arranged by size: 3796 1730 952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3715 3833 844 Size: 2871 118 3489 Fragments arranged by size: 3489 2871 118 BsrBI CCG'CTC Cuts at: 1394 1823 2122 4365 1394 Size: 429 299 2243 3507 Fragments arranged by size: 3507 2243 429 299 BsrDI GCAATG_nn' Cuts at: 65 1248 2177 4973 5147 65 Size: 1183 929 2796 174 1396 Fragments arranged by size: 2796 1396 1183 929 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5122 5309 1700 Size: 110 3312 187 2869 Fragments arranged by size: 3312 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6478 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6478 BssSI C'ACGA_G Cuts at: 4538 5550 4538 Size: 1012 5466 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 794 Size: 1301 5177 BstBI TT'CG_AA Cuts at: 2524 5282 2524 Size: 2758 3720 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6478 BstXI CCAn_nnnn'nTGG Cuts at: 2667 2667 Size: 6478 BstYI r'GATC_y Cuts at: 1972 3808 4175 4560 4577 6029 6115 6127 6473 Size: 1836 367 385 17 1452 86 12 346 Cuts at: 6473 1972 Size: 1977 Fragments arranged by size: 1977 1836 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3369 513 Size: 552 939 1365 3622 Fragments arranged by size: 3622 1365 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5739 Fragments arranged by size: 5739 683 56 BtsI GCAGTG_nn' Cuts at: 2037 3516 3994 4788 4808 2037 Size: 1479 478 794 20 3707 Fragments arranged by size: 3707 1479 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3741 3776 529 Size: 35 3177 35 3231 Fragments arranged by size: 3231 3177 35 35 DraI TTT'AAA Cuts at: 3782 3782 Size: 6478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5485 809 Size: 507 4169 1802 Fragments arranged by size: 4169 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2253 3925 Size: 54 1425 50 275 18 357 66 1672 Cuts at: 3925 4814 6443 8 Size: 889 1629 43 Fragments arranged by size: 1672 1629 1425 889 357 275 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3925 1537 Size: 275 2113 4090 Fragments arranged by size: 4090 2113 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4408 1353 Size: 3055 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 2196 5065 5439 5585 387 Size: 1064 138 579 28 2869 374 146 1280 Fragments arranged by size: 2869 1280 1064 579 374 146 138 28 EcoICRI GAG'CTC Cuts at: 2458 2772 2458 Size: 314 6164 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2663 4541 5138 5925 6193 1239 Size: 393 750 281 1878 597 787 268 1524 Fragments arranged by size: 1878 1524 787 750 597 393 281 268 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2255 Size: 6478 EcoO109I rG'GnC_Cy Cuts at: 2125 2402 2725 2125 Size: 277 323 5878 Fragments arranged by size: 5878 323 277 EcoRI G'AATT_C Cuts at: 3787 4165 3787 Size: 378 6100 EcoRV GAT'ATC Cuts at: 2354 3475 2354 Size: 1121 5357 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 6478 FspI TGC'GCA Cuts at: 4984 4984 Size: 6478 HaeII r_GCGC'y Cuts at: 1678 2039 2438 5625 1678 Size: 361 399 3187 2531 Fragments arranged by size: 3187 2531 399 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 5119 5151 5193 Size: 32 135 32 1307 32 2208 32 42 Cuts at: 5193 5225 1373 Size: 32 2626 Fragments arranged by size: 2626 2208 1307 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3169 3426 3822 4068 669 Size: 988 281 1231 257 396 246 3079 Fragments arranged by size: 3079 1231 988 396 281 257 246 HindIII A'AGCT_T Cuts at: 748 3410 3570 748 Size: 2662 160 3656 Fragments arranged by size: 3656 2662 160 HpaI GTT'AAC Cuts at: 4068 4068 Size: 6478 KasI G'GCGC_C Cuts at: 2035 2035 Size: 6478 KpnI G_GTAC'C Cuts at: 3803 3803 Size: 6478 MfeI C'AATT_G Cuts at: 4077 4077 Size: 6478 MluI A'CGCG_T Cuts at: 6354 6354 Size: 6478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2922 5592 5776 1305 Size: 1617 2670 184 2007 Fragments arranged by size: 2670 2007 1617 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 4233 Fragments arranged by size: 4233 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2669 2753 3116 3589 3683 4436 Size: 648 1389 114 84 363 473 94 753 Cuts at: 4436 4795 4954 518 Size: 359 159 2042 Fragments arranged by size: 2042 1389 753 648 473 363 359 159 114 94 84 MspA1I CmG'CkG Cuts at: 1606 3894 4571 5719 5964 1606 Size: 2288 677 1148 245 2120 Fragments arranged by size: 2288 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2036 2036 Size: 6478 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4987 Fragments arranged by size: 4987 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6478 NotI GC'GGCC_GC Cuts at: 3925 3925 Size: 6478 NspI r_CATG'y Cuts at: 1132 1229 3158 3292 3836 5381 1132 Size: 97 1929 134 544 1545 2229 Fragments arranged by size: 2229 1929 1545 544 134 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6478 PciI A'CATG_T Cuts at: 5377 5377 Size: 6478 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 5350 PmeI GTTT'AAAC Cuts at: 3782 3782 Size: 6478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4547 4579 6060 6092 4547 Size: 32 1481 32 4933 Fragments arranged by size: 4933 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6478 PsiI TTA'TAA Cuts at: 4048 4048 Size: 6478 PspOMI G'GGCC_C Cuts at: 3258 3258 Size: 6478 PstI C_TGCA'G Cuts at: 830 3830 830 Size: 3000 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6478 SacI G_AGCT'C Cuts at: 2460 2774 2460 Size: 314 6164 SalI G'TCGA_C Cuts at: 1936 3820 1936 Size: 1884 4594 SbfI CC_TGCA'GG Cuts at: 3830 3830 Size: 6478 ScaI AGT'ACT Cuts at: 3542 4726 6438 3542 Size: 1184 1712 3582 Fragments arranged by size: 3582 1712 1184 SfcI C'TryA_G Cuts at: 826 1045 3181 3647 3826 4961 5642 5833 826 Size: 219 2136 466 179 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 466 219 191 179 SfoI GGC'GCC Cuts at: 2037 2037 Size: 6478 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6478 SmaI CCC'GGG Cuts at: 1872 2117 3614 3805 1872 Size: 245 1497 191 4545 Fragments arranged by size: 4545 1497 245 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 2478 3221 4582 5483 5745 820 Size: 197 926 535 743 1361 901 262 1553 Fragments arranged by size: 1553 1361 926 901 743 535 262 197 SnaBI TAC'GTA Cuts at: 493 4269 493 Size: 3776 2702 SpeI A'CTAG_T Cuts at: 152 3331 152 Size: 3179 3299 SphI G_CATG'C Cuts at: 1132 3292 3836 1132 Size: 2160 544 3774 Fragments arranged by size: 3774 2160 544 SspI AAT'ATT Cuts at: 5 52 4402 5 Size: 47 4350 2081 Fragments arranged by size: 4350 2081 47 StuI AGG'CCT Cuts at: 1517 2261 3629 1517 Size: 744 1368 4366 Fragments arranged by size: 4366 1368 744 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 3661 3917 513 Size: 552 939 252 1405 256 3074 Fragments arranged by size: 3074 1405 939 552 256 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4505 4522 4675 4860 5305 325 Size: 1255 2925 17 153 185 445 1498 Fragments arranged by size: 2925 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3108 3540 4724 Size: 274 80 33 51 1443 1131 432 1184 Cuts at: 4724 6436 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1184 1131 432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6438 6470 6438 Size: 32 6446 Tth111I GACn'n_nGTC Cuts at: 1316 3430 1316 Size: 2114 4364 XbaI T'CTAG_A Cuts at: 3814 3814 Size: 6478 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6478 XhoI C'TCGA_G Cuts at: 1943 2478 1943 Size: 535 5943 XmaI C'CCGG_G Cuts at: 1870 2115 3612 3803 1870 Size: 245 1497 191 4545 Fragments arranged by size: 4545 1497 245 191 XmnI GAAnn'nnTTC Cuts at: 4607 4607 Size: 6478 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4286 276 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI