(Linear) (Six-base) MAPSORT of: KIEE1547.seq Check: 5536 from: 1 to: 1773 KIEE1547.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 1773 Size: 658 1115 AflII C'TTAA_G Cuts at: 0 1216 1773 Size: 1216 557 AflIII A'CryG_T Cuts at: 0 1121 1773 Size: 1121 652 AleI CACnn'nnGTG Cuts at: 0 1584 1678 1773 Size: 1584 94 95 Fragments arranged by size: 1584 95 94 ApaI G_GGCC'C Cuts at: 0 1257 1773 Size: 1257 516 ApaLI G'TGCA_C Cuts at: 0 1577 1773 Size: 1577 196 AvaI C'yCGr_G Cuts at: 0 110 473 1195 1607 1773 Size: 110 363 722 412 166 Fragments arranged by size: 722 412 363 166 110 BanI G'GyrC_C Cuts at: 0 30 1078 1680 1773 Size: 30 1048 602 93 Fragments arranged by size: 1048 602 93 30 BanII G_rGCy'C Cuts at: 0 455 769 1053 1257 1773 Size: 455 314 284 204 516 Fragments arranged by size: 516 455 314 284 204 BbeI G_GCGC'C Cuts at: 0 34 1773 Size: 34 1739 BbsI GAAGACnn'nnnn_ Cuts at: 0 1751 1773 Size: 1751 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1666 1700 1773 Size: 1666 34 73 Fragments arranged by size: 1666 73 34 BclI T'GATC_A Cuts at: 0 617 1773 Size: 617 1156 BlpI GC'TnA_GC Cuts at: 0 454 1773 Size: 454 1319 Bme1580I G_kGCm'C Cuts at: 0 1257 1581 1773 Size: 1257 324 192 Fragments arranged by size: 1257 324 192 BmgBI CAC'GTC Cuts at: 0 1124 1773 Size: 1124 649 BmrI ACTGGGnnnn_n' Cuts at: 0 1437 1527 1773 Size: 1437 90 246 Fragments arranged by size: 1437 246 90 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 469 1773 Size: 469 1304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 1773 Size: 377 1396 Bpu10I CC'TnA_GC Cuts at: 0 393 847 1773 Size: 393 454 926 Fragments arranged by size: 926 454 393 BsaBI GATnn'nnATC Cuts at: 0 965 1773 Size: 965 808 BsaHI Gr'CG_yC Cuts at: 0 31 1773 Size: 31 1742 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 874 904 1773 Size: 874 30 869 Fragments arranged by size: 874 869 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 730 1773 Size: 730 1043 BsiHKAI G_wGCw'C Cuts at: 0 455 769 1581 1773 Size: 455 314 812 192 Fragments arranged by size: 812 455 314 192 Bsp1286I G_dGCh'C Cuts at: 0 455 769 1053 1257 1581 1773 Size: 455 314 284 204 324 192 Fragments arranged by size: 455 324 314 284 204 192 BspHI T'CATG_A Cuts at: 0 1410 1773 Size: 1410 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1710 1773 Size: 1710 63 BsrBI CCG'CTC Cuts at: 0 117 1773 Size: 117 1656 BsrDI GCAATG_nn' Cuts at: 0 172 1773 Size: 172 1601 BstAPI GCAn_nnn'nTGC Cuts at: 0 90 1773 Size: 90 1683 BstBI TT'CG_AA Cuts at: 0 519 1773 Size: 519 1254 BstXI CCAn_nnnn'nTGG Cuts at: 0 662 1773 Size: 662 1111 BtgI C'CryG_G Cuts at: 0 1364 1773 Size: 1364 409 BtsI GCAGTG_nn' Cuts at: 0 32 1511 1773 Size: 32 1479 262 Fragments arranged by size: 1479 262 32 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1736 1771 1773 Size: 1736 35 2 Fragments arranged by size: 1736 35 2 EaeI y'GGCC_r Cuts at: 0 182 248 1773 Size: 182 66 1525 Fragments arranged by size: 1525 182 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 163 191 1773 Size: 163 28 1582 Fragments arranged by size: 1582 163 28 EcoICRI GAG'CTC Cuts at: 0 453 767 1773 Size: 453 314 1006 Fragments arranged by size: 1006 453 314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 658 1773 Size: 377 281 1115 Fragments arranged by size: 1115 377 281 EcoNI CCTnn'n_nnAGG Cuts at: 0 250 1773 Size: 250 1523 EcoO109I rG'GnC_Cy Cuts at: 0 120 397 720 1773 Size: 120 277 323 1053 Fragments arranged by size: 1053 323 277 120 EcoRV GAT'ATC Cuts at: 0 349 1470 1773 Size: 349 1121 303 Fragments arranged by size: 1121 349 303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 276 1773 Size: 276 1497 HaeII r_GCGC'y Cuts at: 0 34 433 1773 Size: 34 399 1340 Fragments arranged by size: 1340 399 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 874 906 1773 Size: 874 32 867 Fragments arranged by size: 874 867 32 HincII GTy'rAC Cuts at: 0 1164 1421 1773 Size: 1164 257 352 Fragments arranged by size: 1164 352 257 HindIII A'AGCT_T Cuts at: 0 1405 1565 1773 Size: 1405 160 208 Fragments arranged by size: 1405 208 160 KasI G'GCGC_C Cuts at: 0 30 1773 Size: 30 1743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 917 1773 Size: 917 856 MscI TGG'CCA Cuts at: 0 250 1773 Size: 250 1523 MslI CAynn'nnrTG Cuts at: 0 550 664 748 1111 1584 1678 1773 Size: 550 114 84 363 473 94 95 Fragments arranged by size: 550 473 363 114 95 94 84 NarI GG'CG_CC Cuts at: 0 31 1773 Size: 31 1742 NspI r_CATG'y Cuts at: 0 1153 1287 1773 Size: 1153 134 486 Fragments arranged by size: 1153 486 134 PflMI CCAn_nnn'nTGG Cuts at: 0 231 1773 Size: 231 1542 PspOMI G'GGCC_C Cuts at: 0 1253 1773 Size: 1253 520 SacI G_AGCT'C Cuts at: 0 455 769 1773 Size: 455 314 1004 Fragments arranged by size: 1004 455 314 ScaI AGT'ACT Cuts at: 0 1537 1773 Size: 1537 236 SfcI C'TryA_G Cuts at: 0 1176 1642 1773 Size: 1176 466 131 Fragments arranged by size: 1176 466 131 SfoI GGC'GCC Cuts at: 0 32 1773 Size: 32 1741 SmaI CCC'GGG Cuts at: 0 112 1609 1773 Size: 112 1497 164 Fragments arranged by size: 1497 164 112 SmlI C'TyrA_G Cuts at: 0 473 1216 1773 Size: 473 743 557 Fragments arranged by size: 743 557 473 SpeI A'CTAG_T Cuts at: 0 1326 1773 Size: 1326 447 SphI G_CATG'C Cuts at: 0 1287 1773 Size: 1287 486 StuI AGG'CCT Cuts at: 0 256 1624 1773 Size: 256 1368 149 Fragments arranged by size: 1368 256 149 StyI C'CwwG_G Cuts at: 0 251 1656 1773 Size: 251 1405 117 Fragments arranged by size: 1405 251 117 TatI w'GTAC_w Cuts at: 0 1103 1535 1773 Size: 1103 432 238 Fragments arranged by size: 1103 432 238 Tth111I GACn'n_nGTC Cuts at: 0 1425 1773 Size: 1425 348 XhoI C'TCGA_G Cuts at: 0 473 1773 Size: 473 1300 XmaI C'CCGG_G Cuts at: 0 110 1607 1773 Size: 110 1497 166 Fragments arranged by size: 1497 166 110 Enzymes that do cut: AcuI AflII AflIII AleI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BclI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstBI BstXI BtgI BtsI CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI NarI NspI PflMI PspOMI SacI ScaI SfcI SfoI SmaI SmlI SpeI SphI StuI StyI TatI Tth111I XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglI BglII BmtI BpuEI BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI TaqII TstI XbaI XcmI XmnI ZraI