################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 795 GGTACC G^GTACC AlwNI 583 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 283 CCTAAGG CC^T[ACGT]AGG Aor51HI 249 AGCGCT AGC^GCT ApaI 53 GGGCCC GGGCC^C Asp718I 795 GGTACC G^GTACC AspHI 631 GAGCTC G[AT]GC[AT]^C AvaI 1149 CTCGGG C^[CT]CG[GA]G BamHI 1038 GGATCC G^GATCC BanI 795 GGTACC G^G[CT][GA]CC BbsI 132 GAAGAC GAAGAC(2/6) BpuAI 132 GAAGAC GAAGAC(2/6) BsaI 664 GAGACC GGTCTC(1/5) BsgI 1376 GTGCAG GTGCAG(16/14) BsiHKAI 631 GAGCTC G[AT]GC[AT]^C BsiYI 149 CCAATATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 149 CCAATATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 886 GCATTC GAATGC(1/-1) BsoBI 1149 CTCGGG C^[CT]CG[GA]G BspHI 1137 TCATGA T^CATGA BstXI 569 CCAAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 283 CCTAAGG CC^T[ACGT]AGG DraII 53 GGGCCCT [GA]G^G[ACGT]CC[CT] Eco47III 249 AGCGCT AGC^GCT Eco81I 283 CCTAAGG CC^T[ACGT]AGG EcoO109I 53 GGGCCCT [GA]G^G[ACGT]CC[CT] HaeII 249 AGCGCT [GA]GCGC^[CT] HphI 404 TCACC GGTGA(8/7) KpnI 795 GGTACC GGTAC^C MaeII 136 ACGT A^CGT MslI 44 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 1070 CATATG CA^TATG PshAI 273 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1137 TCATGA T^CATGA SacI 631 GAGCTC GAGCT^C SmlI 528 CTCAAG C^T[CT][GA]AG TfiI 1183 GATTC G^A[AT]TC XbaI 1089 TCTAGA T^CTAGA XcmI 659 CCAGTGAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaII 272 GGACC G^G[AT]CC 1063 GGACC BanII 53 GGGCCC G[GA]GC[CT]^C 631 GAGCTC BglI 757 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1352 GCCAGTATGGC BmyI 53 GGGCCC G[AGT]GC[ACT]^C 631 GAGCTC BpmI 859 CTGGAG CTGGAG(16/14) 1382 CTGGAG BsmAI 664 GAGAC GTCTC(1/5) 1032 GTCTC Bsp1286I 53 GGGCCC G[AGT]GC[ACT]^C 631 GAGCTC BsrDI 341 GCAATG GCAATG(2/0) 952 GCAATG BssKI 811 CCAGG ^CC[ACGT]GG 1278 CCTGG BstNI 811 CCAGG CC^[AT]GG 1278 CCTGG BstYI 1038 GGATCC [GA]^GATC[CT] 1267 GGATCT Cac8I 370 GCTGGC GC[ACGT]^[ACGT]GC 581 GCCAGC CfoI 250 GCGC GCG^C 741 GCGC DraI 25 TTTAAA TTT^AAA 1171 TTTAAA EcoRII 811 CCAGG ^CC[AT]GG 1278 CCTGG HhaI 250 GCGC GCG^C 741 GCGC HinP1I 250 GCGC G^CGC 741 GCGC HindIII 1142 AAGCTT A^AGCTT 1412 AAGCTT MflI 1038 GGATCC [GA]^GATC[CT] 1267 GGATCT MvaI 811 CCAGG CC^[AT]GG 1278 CCTGG PleI 387 GACTC GAGTC(4/5) 779 GACTC PstI 680 CTGCAG CTGCA^G 1305 CTGCAG ScrFI 811 CCAGG CC^[ACGT]GG 1278 CCTGG SfcI 680 CTGCAG C^T[GA][CT]AG 1305 CTGCAG XhoII 1038 GGATCC [GA]^GATC[CT] 1267 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BalI BbvI BfaI BsaJI BsmFI BspMI BsrI BstF5I Cfr13I DdeI DpnII DsaI EaeI EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MwoI NcoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI