(Circular) (Six-base) MAPSORT of: pFN21AE4456.seq Check: 9314 from: 1 to: 6130 pFN21AE4456 6130 bp With 182 enzymes: SgfI * March 28, 2012 13:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2483 Size: 6130 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 2800 3451 2800 Size: 651 5479 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4193 5577 5845 1632 Size: 2561 1384 268 1917 Fragments arranged by size: 2561 1917 1384 268 AfeI AGC'GCT Cuts at: 2256 2256 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3417 1184 Size: 2233 3897 AlwNI CAG_nnn'CTG Cuts at: 2593 3873 5445 2593 Size: 1280 1572 3278 Fragments arranged by size: 3278 1572 1280 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 6130 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2609 2926 3137 3172 3439 3665 3817 5926 Size: 1116 317 211 35 267 226 152 2109 Cuts at: 5926 1493 Size: 1697 Fragments arranged by size: 2109 1697 1116 317 267 226 211 152 35 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3154 3455 1870 Size: 73 1211 301 4545 Fragments arranged by size: 4545 1211 301 73 BamHI G'GATC_C Cuts at: 3043 3460 3827 3043 Size: 417 367 5346 Fragments arranged by size: 5346 417 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2800 3451 4906 618 Size: 325 217 627 1013 651 1455 1842 Fragments arranged by size: 1842 1455 1013 651 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2640 3449 1391 Size: 671 578 809 4072 Fragments arranged by size: 4072 809 671 578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 928 Size: 971 245 4914 Fragments arranged by size: 4914 971 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2768 3363 4741 Size: 107 122 71 631 605 1096 595 1378 Cuts at: 4741 136 Size: 1525 Fragments arranged by size: 1525 1378 1096 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2062 4191 5347 1790 Size: 272 2129 1156 2573 Fragments arranged by size: 2573 2129 1156 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3274 3861 4819 Size: 416 53 83 399 24 1857 587 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1857 1753 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2885 3408 4790 1239 Size: 1646 523 1382 2579 Fragments arranged by size: 2579 1646 1382 523 Bpu10I CC'TnA_GC Cuts at: 2634 2634 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2518 4255 5120 5418 2518 Size: 1737 865 298 3230 Fragments arranged by size: 3230 1737 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2663 4793 882 Size: 617 1164 2130 2219 Fragments arranged by size: 2219 2130 1164 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2652 1178 Size: 30 165 30 1219 30 4656 Fragments arranged by size: 4656 1219 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 2097 2113 5333 5829 2097 Size: 16 3220 496 2398 Fragments arranged by size: 3220 2398 496 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3402 1163 Size: 573 1666 3891 Fragments arranged by size: 3891 1666 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2640 4191 4276 5347 2640 Size: 1551 85 1071 3423 Fragments arranged by size: 3423 1551 1071 85 BsmI GAATG_Cn' Cuts at: 2891 3641 3734 2891 Size: 750 93 5287 Fragments arranged by size: 5287 750 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2640 3449 4191 4276 5347 1391 Size: 399 272 578 809 742 85 1071 2174 Fragments arranged by size: 2174 1071 809 742 578 399 272 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 3142 4019 5749 3142 Size: 877 1730 3523 Fragments arranged by size: 3523 1730 877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 2896 3303 3485 844 Size: 1639 413 407 182 3489 Fragments arranged by size: 3489 1639 413 407 182 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2353 2964 4625 4799 65 Size: 1183 1105 611 1661 174 1396 Fragments arranged by size: 1661 1396 1183 1105 611 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 6130 BstYI r'GATC_y Cuts at: 1972 3043 3272 3460 3827 4212 4229 5681 5767 Size: 1071 229 188 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 229 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2289 2289 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2412 2673 2991 3183 513 Size: 552 1347 261 318 192 3460 Fragments arranged by size: 3460 1347 552 318 261 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2043 2741 3646 4440 4460 2043 Size: 698 905 794 20 3713 Fragments arranged by size: 3713 905 794 698 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2223 2258 2331 2366 2434 2469 529 Size: 35 1659 35 73 35 68 35 4190 Fragments arranged by size: 4190 1659 73 68 35 35 35 35 DraI TTT'AAA Cuts at: 2032 3178 3434 2032 Size: 1146 256 4728 Fragments arranged by size: 4728 1146 256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2470 2697 2874 Size: 54 1425 50 275 18 640 227 177 Cuts at: 2874 2988 3577 4466 6095 8 Size: 114 589 889 1629 43 Fragments arranged by size: 1629 1425 889 640 589 275 227 177 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4060 1353 Size: 2707 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2987 4717 5091 5237 387 Size: 1064 138 1398 1730 374 146 1280 Fragments arranged by size: 1730 1398 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2638 2638 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2885 3408 4193 4790 5577 5845 1239 Size: 393 1253 523 785 597 787 268 1524 Fragments arranged by size: 1524 1253 787 785 597 523 393 268 EcoO109I rG'GnC_Cy Cuts at: 2059 2059 Size: 6130 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2804 2804 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2258 5277 1678 Size: 580 3019 2531 Fragments arranged by size: 3019 2531 580 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2383 2415 2495 2527 2545 Size: 32 135 32 811 32 80 32 18 Cuts at: 2545 2577 2936 2968 4771 4803 4845 4877 1373 Size: 32 359 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 811 359 135 80 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 3147 3417 748 Size: 2399 270 3461 Fragments arranged by size: 3461 2399 270 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 2804 3455 2804 Size: 651 5479 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3214 5244 5428 1305 Size: 1909 2030 184 2007 Fragments arranged by size: 2030 2007 1909 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2472 2876 2990 10 Size: 54 1425 343 640 404 114 3150 Fragments arranged by size: 3150 1425 640 404 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2053 4088 4447 4606 518 Size: 648 887 2035 359 159 2042 Fragments arranged by size: 2042 2035 887 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2117 2702 2921 3386 3546 4223 5371 5616 Size: 511 585 219 465 160 677 1148 245 Cuts at: 5616 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 585 511 465 245 219 160 NcoI C'CATG_G Cuts at: 513 1065 2412 2673 2991 3183 513 Size: 552 1347 261 318 192 3460 Fragments arranged by size: 3460 1347 552 318 261 192 NdeI CA'TA_TG Cuts at: 387 3076 387 Size: 2689 3441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3283 1348 Size: 1935 4195 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2282 1613 Size: 669 5461 PsiI TTA'TAA Cuts at: 3000 3700 3000 Size: 700 5430 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 6130 PstI C_TGCA'G Cuts at: 830 2689 3314 3482 830 Size: 1859 625 168 3478 Fragments arranged by size: 3478 1859 625 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2117 2921 3386 2117 Size: 804 465 4861 Fragments arranged by size: 4861 804 465 SacI G_AGCT'C Cuts at: 2640 2640 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2685 3310 3478 4613 5294 5485 826 Size: 219 1640 625 168 1135 681 191 1471 Fragments arranged by size: 1640 1471 1135 681 625 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2533 4234 5135 5397 820 Size: 197 926 590 1701 901 262 1553 Fragments arranged by size: 1701 1553 926 901 590 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2368 2412 2673 2991 3183 3228 3569 Size: 552 1303 44 261 318 192 45 341 Cuts at: 3569 513 Size: 3074 Fragments arranged by size: 3074 1303 552 341 318 261 192 45 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 3306 4157 4174 4327 4512 4957 Size: 1255 1126 600 851 17 153 185 445 Cuts at: 4957 325 Size: 1498 Fragments arranged by size: 1498 1255 1126 851 600 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4376 6088 96 Size: 274 80 33 51 1443 2399 1712 138 Fragments arranged by size: 2399 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 6090 6122 2622 Size: 32 3436 32 2630 Fragments arranged by size: 3436 2630 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3094 3466 3094 Size: 372 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 2671 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI