(Linear) (Six-base) MAPSORT of: KIEE4456.seq Check: 3231 from: 1 to: 1425 KIEE4456.orf With 182 enzymes: SgfI * March 22, 2012 18:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 478 1425 Size: 478 947 Acc65I G'GTAC_C Cuts at: 0 795 1425 Size: 795 630 AfeI AGC'GCT Cuts at: 0 251 1425 Size: 251 1174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1412 1425 Size: 1412 13 AlwNI CAG_nnn'CTG Cuts at: 0 588 1425 Size: 588 837 ApaI G_GGCC'C Cuts at: 0 57 1425 Size: 57 1368 ApoI r'AATT_y Cuts at: 0 604 921 1132 1167 1425 Size: 604 317 211 35 258 Fragments arranged by size: 604 317 258 211 35 AvaI C'yCGr_G Cuts at: 0 1149 1425 Size: 1149 276 BamHI G'GATC_C Cuts at: 0 1038 1425 Size: 1038 387 BanI G'GyrC_C Cuts at: 0 795 1425 Size: 795 630 BanII G_rGCy'C Cuts at: 0 57 635 1425 Size: 57 578 790 Fragments arranged by size: 790 578 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 139 1425 Size: 139 1286 BglI GCCn_nnn'nGGC Cuts at: 0 763 1358 1425 Size: 763 595 67 Fragments arranged by size: 763 595 67 Bme1580I G_kGCm'C Cuts at: 0 57 1425 Size: 57 1368 BmrI ACTGGGnnnn_n' Cuts at: 0 1269 1425 Size: 1269 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1403 1425 Size: 880 523 22 Fragments arranged by size: 880 523 22 Bpu10I CC'TnA_GC Cuts at: 0 629 1425 Size: 629 796 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 1425 Size: 513 912 BsaI GGTCTCn'nnnn_ Cuts at: 0 658 1425 Size: 658 767 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 617 647 1425 Size: 617 30 778 Fragments arranged by size: 778 617 30 BseYI C'CCAG_C Cuts at: 0 92 108 1425 Size: 92 16 1317 Fragments arranged by size: 1317 92 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1397 1425 Size: 1397 28 BsiHKAI G_wGCw'C Cuts at: 0 635 1425 Size: 635 790 BsmI GAATG_Cn' Cuts at: 0 886 1425 Size: 886 539 Bsp1286I G_dGCh'C Cuts at: 0 57 635 1425 Size: 57 578 790 Fragments arranged by size: 790 578 57 BspHI T'CATG_A Cuts at: 0 1137 1425 Size: 1137 288 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 478 891 1298 1425 Size: 478 413 407 127 Fragments arranged by size: 478 413 407 127 BsrDI GCAATG_nn' Cuts at: 0 348 959 1425 Size: 348 611 466 Fragments arranged by size: 611 466 348 BstXI CCAn_nnnn'nTGG Cuts at: 0 576 1425 Size: 576 849 BstYI r'GATC_y Cuts at: 0 1038 1267 1425 Size: 1038 229 158 Fragments arranged by size: 1038 229 158 Bsu36I CC'TnA_GG Cuts at: 0 284 1425 Size: 284 1141 BtgI C'CryG_G Cuts at: 0 407 668 986 1178 1425 Size: 407 261 318 192 247 Fragments arranged by size: 407 318 261 247 192 BtsI GCAGTG_nn' Cuts at: 0 38 736 1425 Size: 38 698 689 Fragments arranged by size: 698 689 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 218 253 326 361 429 464 1425 Size: 218 35 73 35 68 35 961 Fragments arranged by size: 961 218 73 68 35 35 35 DraI TTT'AAA Cuts at: 0 27 1173 1425 Size: 27 1146 252 Fragments arranged by size: 1146 252 27 EaeI y'GGCC_r Cuts at: 0 465 692 869 983 1425 Size: 465 227 177 114 442 Fragments arranged by size: 465 442 227 177 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 982 1425 Size: 982 443 EcoICRI GAG'CTC Cuts at: 0 633 1425 Size: 633 792 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1403 1425 Size: 880 523 22 Fragments arranged by size: 880 523 22 EcoO109I rG'GnC_Cy Cuts at: 0 54 1425 Size: 54 1371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 799 1425 Size: 799 626 HaeII r_GCGC'y Cuts at: 0 253 1425 Size: 253 1172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 378 410 490 522 540 572 931 963 Size: 378 32 80 32 18 32 359 32 Cuts at: 963 1425 Size: 462 Fragments arranged by size: 462 378 359 80 32 32 32 32 18 HindIII A'AGCT_T Cuts at: 0 1142 1412 1425 Size: 1142 270 13 Fragments arranged by size: 1142 270 13 KpnI G_GTAC'C Cuts at: 0 799 1425 Size: 799 626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1209 1425 Size: 1209 216 MscI TGG'CCA Cuts at: 0 467 871 985 1425 Size: 467 404 114 440 Fragments arranged by size: 467 440 404 114 MslI CAynn'nnrTG Cuts at: 0 48 1425 Size: 48 1377 MspA1I CmG'CkG Cuts at: 0 112 697 916 1381 1425 Size: 112 585 219 465 44 Fragments arranged by size: 585 465 219 112 44 NcoI C'CATG_G Cuts at: 0 407 668 986 1178 1425 Size: 407 261 318 192 247 Fragments arranged by size: 407 318 261 247 192 NdeI CA'TA_TG Cuts at: 0 1071 1425 Size: 1071 354 PasI CC'CwG_GG Cuts at: 0 1278 1425 Size: 1278 147 PshAI GACnn'nnGTC Cuts at: 0 277 1425 Size: 277 1148 PsiI TTA'TAA Cuts at: 0 995 1425 Size: 995 430 PspOMI G'GGCC_C Cuts at: 0 53 1425 Size: 53 1372 PstI C_TGCA'G Cuts at: 0 684 1309 1425 Size: 684 625 116 Fragments arranged by size: 684 625 116 PvuII CAG'CTG Cuts at: 0 112 916 1381 1425 Size: 112 804 465 44 Fragments arranged by size: 804 465 112 44 SacI G_AGCT'C Cuts at: 0 635 1425 Size: 635 790 SfcI C'TryA_G Cuts at: 0 680 1305 1425 Size: 680 625 120 Fragments arranged by size: 680 625 120 SmlI C'TyrA_G Cuts at: 0 528 1425 Size: 528 897 StyI C'CwwG_G Cuts at: 0 363 407 668 986 1178 1223 1425 Size: 363 44 261 318 192 45 202 Fragments arranged by size: 363 318 261 202 192 45 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 701 1301 1425 Size: 701 600 124 Fragments arranged by size: 701 600 124 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 617 649 1425 Size: 617 32 776 Fragments arranged by size: 776 617 32 XbaI T'CTAG_A Cuts at: 0 1089 1425 Size: 1089 336 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 666 1425 Size: 666 759 Enzymes that do cut: AarI Acc65I AfeI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI PasI PshAI PsiI PspOMI PstI PvuII SacI SfcI SmlI StyI TaqII TstI XbaI XcmI Enzymes that do not cut: AatII AccI AclI AcuI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XhoI XmaI XmnI ZraI