Restriction map for
pFN21AB6314
(Linear) (Six-base) MAPSORT of: KIBB6314.seq Check: 5186 from: 1 to: 843
KIBB6314.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 349 584 632 843
Size: 349 235 48 211
Fragments arranged by size:
349 235 211 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 843
Size: 232 611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 843
Size: 628 215
AlwNI CAG_nnn'CTG
Cuts at: 0 663 843
Size: 663 180
ApaI G_GGCC'C
Cuts at: 0 72 720 843
Size: 72 648 123
Fragments arranged by size:
648 123 72
BanII G_rGCy'C
Cuts at: 0 72 720 791 843
Size: 72 648 71 52
Fragments arranged by size:
648 72 71 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 843
Size: 494 349
BbvCI CC'TCA_GC
Cuts at: 0 427 843
Size: 427 416
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 843
Size: 662 181
BclI T'GATC_A
Cuts at: 0 545 762 843
Size: 545 217 81
Fragments arranged by size:
545 217 81
BglI GCCn_nnn'nGGC
Cuts at: 0 114 843
Size: 114 729
BglII A'GATC_T
Cuts at: 0 32 843
Size: 32 811
Bme1580I G_kGCm'C
Cuts at: 0 72 110 720 843
Size: 72 38 610 123
Fragments arranged by size:
610 123 72 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 395 566 713 843
Size: 48 347 171 147 130
Fragments arranged by size:
347 171 147 130 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 536 843
Size: 59 477 307
Fragments arranged by size:
477 307 59
Bpu10I CC'TnA_GC
Cuts at: 0 427 619 843
Size: 427 192 224
Fragments arranged by size:
427 224 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 843
Size: 447 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 843
Size: 681 162
BsiHKAI G_wGCw'C
Cuts at: 0 791 843
Size: 791 52
Bsp1286I G_dGCh'C
Cuts at: 0 72 110 720 791 843
Size: 72 38 610 71 52
Fragments arranged by size:
610 72 71 52 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 843
Size: 620 223
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 843
Size: 477 366
BstYI r'GATC_y
Cuts at: 0 32 660 843
Size: 32 628 183
Fragments arranged by size:
628 183 32
BstZ17I GTA'TAC
Cuts at: 0 585 843
Size: 585 258
BtsI GCAGTG_nn'
Cuts at: 0 57 184 746 843
Size: 57 127 562 97
Fragments arranged by size:
562 127 97 57
EarI CTCTTCn'nnn_
Cuts at: 0 211 316 843
Size: 211 105 527
Fragments arranged by size:
527 211 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 843
Size: 158 685
EcoICRI GAG'CTC
Cuts at: 0 789 843
Size: 789 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 232 536 843
Size: 59 173 304 307
Fragments arranged by size:
307 304 173 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 843
Size: 322 521
EcoO109I rG'GnC_Cy
Cuts at: 0 69 843
Size: 69 774
HincII GTy'rAC
Cuts at: 0 777 843
Size: 777 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 482 843
Size: 121 361 361
Fragments arranged by size:
361 361 121
MslI CAynn'nnrTG
Cuts at: 0 770 843
Size: 770 73
MspA1I CmG'CkG
Cuts at: 0 201 843
Size: 201 642
PasI CC'CwG_GG
Cuts at: 0 65 843
Size: 65 778
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 843
Size: 663 180
PspOMI G'GGCC_C
Cuts at: 0 68 716 843
Size: 68 648 127
Fragments arranged by size:
648 127 68
SacI G_AGCT'C
Cuts at: 0 791 843
Size: 791 52
SapI GCTCTTCn'nnn_
Cuts at: 0 316 843
Size: 316 527
SfcI C'TryA_G
Cuts at: 0 319 843
Size: 319 524
SmlI C'TyrA_G
Cuts at: 0 462 843
Size: 462 381
StuI AGG'CCT
Cuts at: 0 117 239 843
Size: 117 122 604
Fragments arranged by size:
604 122 117
StyI C'CwwG_G
Cuts at: 0 278 843
Size: 278 565
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 843
Size: 30 813
TatI w'GTAC_w
Cuts at: 0 563 843
Size: 563 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 72 104 843
Size: 72 32 739
Fragments arranged by size:
739 72 32
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI BanII BbsI BbvCI
BciVI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsgI BsiHKAI Bsp1286I BspMI BstXI BstYI BstZ17I
BtsI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HincII
MmeI MslI MspA1I PasI PflMI PspOMI SacI SapI
SfcI SmlI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BfrBI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI