Restriction map for
pFN21AB5888
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MluI MluNI MroI MscI
MspA1I NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 1510 GGTACC G^GTACC
AccI 778 GTAGAC GT^[AC][GT]AC
AccII 58 CGCG CG^CG
AcsI 1552 GAATTT [GA]^AATT[CT]
ApoI 1552 GAATTT [GA]^AATT[CT]
AseI 1104 ATTAAT AT^TAAT
AsnI 1104 ATTAAT AT^TAAT
Asp718I 1510 GGTACC G^GTACC
BamHI 693 GGATCC G^GATCC
BanII 791 GGGCTC G[GA]GC[CT]^C
BsaJI 956 CCCAGG C^C[ACGT][ACGT]GG
BsmI 234 GAATGC GAATGC(1/-1)
Bsp1407I 1221 TGTACA T^GTACA
BsrFI 1324 GCCGGT [GA]^CCGG[CT]
BsrGI 1221 TGTACA T^GTACA
BstUI 58 CGCG CG^CG
Cfr10I 1324 GCCGGT [GA]^CCGG[CT]
DraI 196 TTTAAA TTT^AAA
EarI 608 CTCTTC CTCTTC(1/4)
HapII 1325 CCGG C^CGG
HpaII 1325 CCGG C^CGG
KpnI 1510 GGTACC GGTAC^C
Ksp632I 608 CTCTTC CTCTTC(1/4)
MaeII 656 ACGT A^CGT
MspI 1325 CCGG C^CGG
MvnI 58 CGCG CG^CG
PleI 447 GACTC GAGTC(4/5)
PshBI 1104 ATTAAT AT^TAAT
PstI 681 CTGCAG CTGCA^G
SfcI 681 CTGCAG C^T[GA][CT]AG
SspBI 1221 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 258 GGTCC G^G[AT]CC
562 GGTCC
BanI 940 GGCACC G^G[CT][GA]CC
1510 GGTACC
BbsI 77 GAAGAC GAAGAC(2/6)
1411 GAAGAC
BpuAI 77 GAAGAC GAAGAC(2/6)
1411 GAAGAC
BsaBI 376 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1471 GATGAAGATC
BsgI 440 CTGCAC GTGCAG(16/14)
460 GTGCAG
BsmAI 489 GAGAC GTCTC(1/5)
712 GAGAC
BsmFI 1139 GGGAC GGGAC(10/14)
626 GTCCC
BssKI 276 CCAGG ^CC[ACGT]GG
957 CCAGG
BstF5I 633 CATCC GGATG(2/0)
375 GGATG
BstNI 276 CCAGG CC^[AT]GG
957 CCAGG
BstYI 693 GGATCC [GA]^GATC[CT]
1476 AGATCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
1322 TGGCCG
EcoRII 276 CCAGG ^CC[AT]GG
957 CCAGG
FokI 633 CATCC GGATG(9/13)
375 GGATG
MamI 376 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1471 GATGAAGATC
MfeI 262 CAATTG C^AATTG
969 CAATTG
MflI 693 GGATCC [GA]^GATC[CT]
1476 AGATCC
MunI 262 CAATTG C^AATTG
969 CAATTG
MvaI 276 CCAGG CC^[AT]GG
957 CCAGG
NlaIII 662 CATG CATG^
1402 CATG
ScrFI 276 CCAGG CC^[ACGT]GG
957 CCAGG
SmlI 449 CTCAAG C^T[CT][GA]AG
1274 CTCAAG
XhoII 693 GGATCC [GA]^GATC[CT]
1476 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BsiYI BslI Bsp1286I
BsrI Cac8I Cfr13I DdeI DpnII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIV
RsaI Sau3AI Sau96I SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI