(Circular) (Six-base) MAPSORT of: pFN21AB5888.seq Check: 345 from: 1 to: 6268 pFN21AB5888 6268 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 3611 1937 Size: 847 827 4594 Fragments arranged by size: 4594 847 827 Acc65I G'GTAC_C Cuts at: 3515 3589 3515 Size: 74 6194 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3519 4331 5715 5983 1632 Size: 1887 812 1384 268 1917 Fragments arranged by size: 1917 1887 1384 812 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2490 1184 Size: 1306 4962 AlwNI CAG_nnn'CTG Cuts at: 4011 5583 4011 Size: 1572 4696 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 3557 3577 3803 3955 6064 1493 Size: 2064 20 226 152 2109 1697 Fragments arranged by size: 2109 2064 1697 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 3110 4822 160 Size: 872 2078 1712 1606 Fragments arranged by size: 2078 1712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3593 1870 Size: 73 1650 4545 Fragments arranged by size: 4545 1650 73 BamHI G'GATC_C Cuts at: 2698 3598 3965 2698 Size: 900 367 5001 Fragments arranged by size: 5001 900 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2945 3515 3589 5044 618 Size: 325 217 627 1158 570 74 1455 1842 Fragments arranged by size: 1842 1455 1158 627 570 325 217 74 BanII G_rGCy'C Cuts at: 1391 2800 3587 1391 Size: 1409 787 4072 Fragments arranged by size: 4072 1409 787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 3423 928 Size: 971 190 1334 3773 Fragments arranged by size: 3773 1334 971 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4879 136 Size: 107 122 71 631 605 3207 1525 Fragments arranged by size: 3207 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3685 3685 Size: 6268 Bme1580I G_kGCm'C Cuts at: 1790 2865 2877 4329 5485 1790 Size: 1075 12 1452 1156 2573 Fragments arranged by size: 2573 1452 1156 1075 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2252 2606 3999 Size: 416 53 83 399 24 835 354 1393 Cuts at: 3999 4957 442 Size: 958 1753 Fragments arranged by size: 1753 1393 958 835 416 399 354 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4928 1239 Size: 3689 2579 Bpu10I CC'TnA_GC Cuts at: 3138 3138 Size: 6268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2439 3264 4393 5258 5556 2439 Size: 825 1129 865 298 3151 Fragments arranged by size: 3151 1129 865 825 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4931 882 Size: 617 3432 2219 Fragments arranged by size: 3432 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 2385 3480 6262 2385 Size: 1095 2782 2391 Fragments arranged by size: 2782 2391 1095 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4701 5099 5373 5520 5931 4701 Size: 398 274 147 411 5038 Fragments arranged by size: 5038 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6043 Fragments arranged by size: 6043 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6268 BseYI C'CCAG_C Cuts at: 2018 5471 5967 2018 Size: 3453 496 2319 Fragments arranged by size: 3453 2319 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2430 2486 1163 Size: 573 694 56 4945 Fragments arranged by size: 4945 694 573 56 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 2245 3779 3872 2245 Size: 1534 93 4641 Fragments arranged by size: 4641 1534 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2800 2865 2877 3587 4329 4414 5485 Size: 399 1010 65 12 710 742 85 1071 Cuts at: 5485 1391 Size: 2174 Fragments arranged by size: 2174 1071 1010 742 710 399 85 65 12 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3623 844 Size: 2779 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4763 4937 65 Size: 1183 3515 174 1396 Fragments arranged by size: 3515 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3329 4912 5099 1700 Size: 110 1519 1583 187 2869 Fragments arranged by size: 2869 1583 1519 187 110 BsrGI T'GTAC_A Cuts at: 96 3226 96 Size: 3130 3138 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstYI r'GATC_y Cuts at: 1972 2698 3481 3598 3965 4350 4367 5819 5905 Size: 726 783 117 367 385 17 1452 86 Cuts at: 5905 5917 6263 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 783 726 385 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2079 487 Size: 683 56 853 4676 Fragments arranged by size: 4676 853 683 56 BtsI GCAGTG_nn' Cuts at: 3048 3784 4578 4598 3048 Size: 736 794 20 4718 Fragments arranged by size: 4718 794 736 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 2203 3572 2203 Size: 1369 4899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3327 3715 Size: 54 1425 50 275 18 177 1320 388 Cuts at: 3715 4604 6233 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1320 889 388 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 4198 1353 Size: 1266 1579 3423 Fragments arranged by size: 3423 1579 1266 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4855 5229 5375 387 Size: 1064 138 3266 374 146 1280 Fragments arranged by size: 3266 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3519 4331 4928 5715 5983 1239 Size: 393 1887 812 597 787 268 1524 Fragments arranged by size: 1887 1524 812 787 597 393 268 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3163 3163 Size: 6268 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 5415 1678 Size: 3737 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2571 2603 2972 3004 3112 Size: 32 135 32 999 32 369 32 108 Cuts at: 3112 3144 4909 4941 4983 5015 1373 Size: 32 1765 32 42 32 2626 Fragments arranged by size: 2626 1765 999 369 135 108 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3612 3858 669 Size: 988 281 1674 246 3079 Fragments arranged by size: 3079 1674 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6268 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3519 3593 3519 Size: 74 6194 MfeI C'AATT_G Cuts at: 2267 2974 3867 2267 Size: 707 893 4668 Fragments arranged by size: 4668 893 707 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3081 3557 5382 5566 1305 Size: 1776 476 1825 184 2007 Fragments arranged by size: 2007 1825 1776 476 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4446 Fragments arranged by size: 4446 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2091 2517 3411 4226 4585 4744 518 Size: 648 925 426 894 815 359 159 2042 Fragments arranged by size: 2042 925 894 815 648 426 359 159 MspA1I CmG'CkG Cuts at: 1606 3684 4361 5509 5754 1606 Size: 2078 677 1148 245 2120 Fragments arranged by size: 2120 2078 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 3626 5171 1132 Size: 97 2397 1545 2229 Fragments arranged by size: 2397 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PstI C_TGCA'G Cuts at: 830 2690 3620 830 Size: 1860 930 3478 Fragments arranged by size: 3478 1860 930 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2686 3616 4751 5432 5623 826 Size: 219 1641 930 1135 681 191 1471 Fragments arranged by size: 1641 1471 1135 930 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2454 3279 4372 5273 5535 820 Size: 197 926 511 825 1093 901 262 1553 Fragments arranged by size: 1553 1093 926 901 825 511 262 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2004 3707 513 Size: 552 939 1703 3074 Fragments arranged by size: 3074 1703 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3030 4295 4312 4465 4650 5095 325 Size: 1255 1450 1265 17 153 185 445 1498 Fragments arranged by size: 1498 1450 1265 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3226 4514 6226 Size: 274 80 33 51 1443 1249 1288 1712 Cuts at: 6226 96 Size: 138 Fragments arranged by size: 1712 1443 1288 1249 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3029 3061 6228 6260 3029 Size: 32 3167 32 3037 Fragments arranged by size: 3167 3037 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6268 XbaI T'CTAG_A Cuts at: 2173 2371 3298 3604 2173 Size: 198 927 306 4837 Fragments arranged by size: 4837 927 306 198 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 4397 4397 Size: 6268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI