################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII ClaI CpoI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 402 GTAC GT^AC AhdI 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 253 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 291 GGGCCC GGGCC^C AspEI 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 394 GGTGCC G^G[CT][GA]CC BanII 291 GGGCCC G[GA]GC[CT]^C BbvCI 145 CCTCAGC CCTCAGC(-5/-2) BclI 219 TGATCA T^GATCA BsaI 436 GAGACC GGTCTC(1/5) BseRI 265 GAGGAG GAGGAG(10/8) BsgI 405 CTGCAC GTGCAG(16/14) BsrFI 86 ACCGGC [GA]^CCGG[CT] BsrI 190 ACTGG ACTGG(1/-1) Cfr10I 86 ACCGGC [GA]^CCGG[CT] DpnII 220 GATC ^GATC DraI 226 TTTAAA TTT^AAA EaeI 284 TGGCCG [CT]^GGCC[GA] Eam1105I 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 258 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 219 TGATCA T^GATCA HaeII 338 AGCGCC [GA]GCGC^[CT] HgaI 75 GCGTC GACGC(5/10) HinfI 140 GACTC G^A[ACGT]TC HphI 217 GGTGA GGTGA(8/7) MboI 220 GATC ^GATC MseI 227 TTAA T^TAA NdeII 220 GATC ^GATC PleI 140 GACTC GAGTC(4/5) RsaI 402 GTAC GT^AC Sau3AI 220 GATC ^GATC SexAI 439 ACCAGGT A^CC[AT]GGT SmaI 37 CCCGGG CCC^GGG SmlI 276 CTTGAG C^T[CT][GA]AG Tru9I 227 TTAA T^TAA XmaCI 37 CCCGGG C^CCGGG XmaI 37 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 135 CGCG CG^CG 329 CGCG AvaI 37 CCCGGG C^[CT]CG[GA]G 212 CCCGAG BmyI 291 GGGCCC G[AGT]GC[ACT]^C 395 GTGCCC BpmI 94 CTGGAG CTGGAG(16/14) 103 CTGGAG BsmAI 159 GAGAC GTCTC(1/5) 436 GAGAC BsoBI 37 CCCGGG C^[CT]CG[GA]G 212 CCCGAG Bsp1286I 291 GGGCCC G[AGT]GC[ACT]^C 395 GTGCCC BstF5I 368 CATCC GGATG(2/0) 123 GGATG BstUI 135 CGCG CG^CG 329 CGCG CfoI 339 GCGC GCG^C 414 GCGC DdeI 146 CTCAG C^T[ACGT]AG 167 CTCAG FokI 368 CATCC GGATG(9/13) 123 GGATG HhaI 339 GCGC GCG^C 414 GCGC HinP1I 339 GCGC G^CGC 414 GCGC MboII 9 GAAGA GAAGA(8/7) 204 GAAGA MvnI 135 CGCG CG^CG 329 CGCG PvuII 91 CAGCTG CAG^CTG 387 CAGCTG SfaNI 181 GATGC GCATC(5/9) 80 GCATC TspRI 189 CACTG CA[GC]TG[ACGT][ACGT]^ 408 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BsmFI BssKI BstNI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIV PpuMI Sau96I ScrFI TseI