(Linear) (Six-base) MAPSORT of: KIEE0497.seq Check: 7955 from: 1 to: 1200 KIEE0497.orf With 182 enzymes: SgfI * February 2, 2010 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 992 1200 Size: 992 208 AatII G_ACGT'C Cuts at: 0 953 1200 Size: 953 247 AccI GT'mk_AC Cuts at: 0 311 1200 Size: 311 889 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 370 832 946 1200 Size: 232 138 462 114 254 Fragments arranged by size: 462 254 232 138 114 AflIII A'CryG_T Cuts at: 0 566 1200 Size: 566 634 AleI CACnn'nnGTG Cuts at: 0 680 706 1200 Size: 680 26 494 Fragments arranged by size: 680 494 26 AlwNI CAG_nnn'CTG Cuts at: 0 477 744 1166 1200 Size: 477 267 422 34 Fragments arranged by size: 477 422 267 34 ApaI G_GGCC'C Cuts at: 0 364 1200 Size: 364 836 ApaLI G'TGCA_C Cuts at: 0 709 1118 1200 Size: 709 409 82 Fragments arranged by size: 709 409 82 AvrII C'CTAG_G Cuts at: 0 1143 1200 Size: 1143 57 BamHI G'GATC_C Cuts at: 0 1011 1200 Size: 1011 189 BanII G_rGCy'C Cuts at: 0 364 515 557 1200 Size: 364 151 42 643 Fragments arranged by size: 643 364 151 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1020 1054 1200 Size: 1020 34 146 Fragments arranged by size: 1020 146 34 BlpI GC'TnA_GC Cuts at: 0 1126 1200 Size: 1126 74 Bme1580I G_kGCm'C Cuts at: 0 364 713 1122 1200 Size: 364 349 409 78 Fragments arranged by size: 409 364 349 78 BmrI ACTGGGnnnn_n' Cuts at: 0 759 924 1200 Size: 759 165 276 Fragments arranged by size: 759 276 165 Bpu10I CC'TnA_GC Cuts at: 0 418 1200 Size: 418 782 BsaAI yAC'GTr Cuts at: 0 612 1200 Size: 612 588 BsaHI Gr'CG_yC Cuts at: 0 950 1200 Size: 950 250 BsaWI w'CCGG_w Cuts at: 0 50 287 1200 Size: 50 237 913 Fragments arranged by size: 913 237 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 217 247 1200 Size: 217 30 953 Fragments arranged by size: 953 217 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 79 151 241 262 537 952 1108 1200 Size: 79 72 90 21 275 415 156 92 Fragments arranged by size: 415 275 156 92 90 79 72 21 BseYI C'CCAG_C Cuts at: 0 689 975 1200 Size: 689 286 225 Fragments arranged by size: 689 286 225 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 1101 1152 1200 Size: 502 599 51 48 Fragments arranged by size: 599 502 51 48 BsiEI CG_ry'CG Cuts at: 0 764 871 1200 Size: 764 107 329 Fragments arranged by size: 764 329 107 BsiHKAI G_wGCw'C Cuts at: 0 557 713 1122 1200 Size: 557 156 409 78 Fragments arranged by size: 557 409 156 78 Bsp1286I G_dGCh'C Cuts at: 0 364 515 557 713 1122 1200 Size: 364 151 42 156 409 78 Fragments arranged by size: 409 364 156 151 78 42 BspEI T'CCGG_A Cuts at: 0 50 287 1200 Size: 50 237 913 Fragments arranged by size: 913 237 50 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 679 899 992 1200 Size: 679 220 93 208 Fragments arranged by size: 679 220 208 93 BsrBI CCG'CTC Cuts at: 0 23 45 1200 Size: 23 22 1155 Fragments arranged by size: 1155 23 22 BsrFI r'CCGG_y Cuts at: 0 143 194 863 1200 Size: 143 51 669 337 Fragments arranged by size: 669 337 143 51 BsrGI T'GTAC_A Cuts at: 0 569 1200 Size: 569 631 BstAPI GCAn_nnn'nTGC Cuts at: 0 1166 1200 Size: 1166 34 BstXI CCAn_nnnn'nTGG Cuts at: 0 303 682 839 1200 Size: 303 379 157 361 Fragments arranged by size: 379 361 303 157 BstYI r'GATC_y Cuts at: 0 986 1011 1200 Size: 986 25 189 Fragments arranged by size: 986 189 25 Bsu36I CC'TnA_GG Cuts at: 0 106 520 1200 Size: 106 414 680 Fragments arranged by size: 680 414 106 BtgI C'CryG_G Cuts at: 0 558 1200 Size: 558 642 BtsI GCAGTG_nn' Cuts at: 0 379 638 901 1200 Size: 379 259 263 299 Fragments arranged by size: 379 299 263 259 EaeI y'GGCC_r Cuts at: 0 152 275 542 868 968 1200 Size: 152 123 267 326 100 232 Fragments arranged by size: 326 267 232 152 123 100 EagI C'GGCC_G Cuts at: 0 868 1200 Size: 868 332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 55 76 136 1200 Size: 55 21 60 1064 Fragments arranged by size: 1064 60 55 21 EcoICRI GAG'CTC Cuts at: 0 555 1200 Size: 555 645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 370 832 946 1200 Size: 232 138 462 114 254 Fragments arranged by size: 462 254 232 138 114 EcoNI CCTnn'n_nnAGG Cuts at: 0 88 109 421 999 1200 Size: 88 21 312 578 201 Fragments arranged by size: 578 312 201 88 21 EcoO109I rG'GnC_Cy Cuts at: 0 207 360 361 686 1098 1200 Size: 207 153 1 325 412 102 Fragments arranged by size: 412 325 207 153 102 1 HaeII r_GCGC'y Cuts at: 0 84 134 284 1200 Size: 84 50 150 916 Fragments arranged by size: 916 150 84 50 HindIII A'AGCT_T Cuts at: 0 874 1200 Size: 874 326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1048 1200 Size: 1048 152 MscI TGG'CCA Cuts at: 0 277 1200 Size: 277 923 MslI CAynn'nnrTG Cuts at: 0 445 680 706 1200 Size: 445 235 26 494 Fragments arranged by size: 494 445 235 26 MspA1I CmG'CkG Cuts at: 0 693 1200 Size: 693 507 NaeI GCC'GGC Cuts at: 0 145 196 865 1200 Size: 145 51 669 335 Fragments arranged by size: 669 335 145 51 NgoMIV G'CCGG_C Cuts at: 0 143 194 863 1200 Size: 143 51 669 337 Fragments arranged by size: 669 337 143 51 NspI r_CATG'y Cuts at: 0 490 570 1200 Size: 490 80 630 Fragments arranged by size: 630 490 80 PciI A'CATG_T Cuts at: 0 566 1200 Size: 566 634 PfoI T'CCnGG_A Cuts at: 0 266 1200 Size: 266 934 PpuMI rG'GwC_Cy Cuts at: 0 686 1200 Size: 686 514 PspOMI G'GGCC_C Cuts at: 0 360 1200 Size: 360 840 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 283 315 1200 Size: 283 32 885 Fragments arranged by size: 885 283 32 PstI C_TGCA'G Cuts at: 0 185 896 1200 Size: 185 711 304 Fragments arranged by size: 711 304 185 PvuII CAG'CTG Cuts at: 0 693 1200 Size: 693 507 SacI G_AGCT'C Cuts at: 0 557 1200 Size: 557 643 SanDI GG'GwC_CC Cuts at: 0 686 1200 Size: 686 514 SexAI A'CCwGG_T Cuts at: 0 167 1200 Size: 167 1033 SfcI C'TryA_G Cuts at: 0 181 892 996 1200 Size: 181 711 104 204 Fragments arranged by size: 711 204 181 104 StyI C'CwwG_G Cuts at: 0 1143 1200 Size: 1143 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 457 778 1200 Size: 457 321 422 Fragments arranged by size: 457 422 321 TatI w'GTAC_w Cuts at: 0 569 1200 Size: 569 631 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 217 249 1200 Size: 217 32 951 Fragments arranged by size: 951 217 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 523 1023 1200 Size: 523 500 177 Fragments arranged by size: 523 500 177 ZraI GAC'GTC Cuts at: 0 951 1200 Size: 951 249 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlwNI ApaI ApaLI AvrII BamHI BanII BcgI BlpI Bme1580I BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SexAI SfcI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvaI BaeI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI