################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MboII MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 567 GTAC GT^AC AlwNI 814 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 548 AGCGCT AGC^GCT AscI 199 GGCGCGCC GG^CGCGCC BfaI 244 CTAG C^TAG BglII 530 AGATCT A^GATCT BpmI 351 CTCCAG CTGGAG(16/14) BsiEI 425 CGGCCG CG[GA][CT]^CG BsmBI 170 CGTCTC CGTCTC(1/5) BsmFI 603 GTCCC GGGAC(10/14) BspMI 624 GCAGGT ACCTGC(4/8) BssSI 37 CACGAG CACGAG(-5/-1) BstAPI 813 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 122 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 238 CTGAG C^T[ACGT]AG DrdI 619 GACGCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 425 CGGCCG C^GGCCG EclXI 425 CGGCCG C^GGCCG Eco47III 548 AGCGCT AGC^GCT Eco52I 425 CGGCCG C^GGCCG KspI 103 CCGCGG CCGC^GG MaeI 244 CTAG C^TAG MaeII 511 ACGT A^CGT MseI 71 TTAA T^TAA NotI 424 GCGGCCGC GC^GGCCGC PleI 760 GACTC GAGTC(4/5) PvuII 724 CAGCTG CAG^CTG RsaI 567 GTAC GT^AC SacII 103 CCGCGG CCGC^GG SexAI 634 ACCTGGT A^CC[AT]GGT TfiI 816 GAATC G^A[AT]TC Tru9I 71 TTAA T^TAA Tsp509I 370 AATT ^AATT TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### ApaI 108 GGGCCC GGGCC^C 136 GGGCCC AvaII 81 GGACC G^G[AT]CC 653 GGACC BsaI 348 GGTCTC GGTCTC(1/5) 364 GGTCTC BsaWI 479 ACCGGA [AT]^CCGG[AT] 647 ACCGGA BseRI 679 GAGGAG GAGGAG(10/8) 832 GAGGAG BsgI 787 CTGCAC GTGCAG(16/14) 526 GTGCAG BsrFI 270 GCCGGC [GA]^CCGG[CT] 710 GCCGGC BstF5I 765 CATCC GGATG(2/0) 380 GGATG Cfr10I 270 GCCGGC [GA]^CCGG[CT] 710 GCCGGC DsaI 103 CCGCGG C^C[GA][CT]GG 344 CCACGG EaeI 425 CGGCCG [CT]^GGCC[GA] 462 CGGCCA EcoT14I 357 CCTTGG C^C[AT][AT]GG 845 CCAAGG FokI 765 CATCC GGATG(9/13) 380 GGATG HinfI 760 GACTC G^A[ACGT]TC 816 GAATC HphI 443 TCACC GGTGA(8/7) 860 TCACC NaeI 270 GCCGGC GCC^GGC 710 GCCGGC NgoMIV 270 GCCGGC G^CCGGC 710 GCCGGC NlaIII 330 CATG CATG^ 717 CATG SfaNI 56 GCATC GCATC(5/9) 458 GCATC StyI 357 CCTTGG C^C[AT][AT]GG 845 CCAAGG TaqI 59 TCGA T^CGA 209 TCGA TthHB8I 59 TCGA T^CGA 209 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrBI BssHII BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MaeIII MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI SfoI SmaI TseI Tsp45I XhoII XmaCI XmaI