(Circular) (Six-base) MAPSORT of: pFN21AB7594.seq Check: 186 from: 1 to: 5578 pFN21AB7594 5578 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3641 5025 5293 1632 Size: 2009 1384 268 1917 Fragments arranged by size: 2009 1917 1384 268 AfeI AGC'GCT Cuts at: 2555 2555 Size: 5578 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AlwNI CAG_nnn'CTG Cuts at: 2824 3321 4893 2824 Size: 497 1572 3509 Fragments arranged by size: 3509 1572 497 ApaI G_GGCC'C Cuts at: 2117 2145 2117 Size: 28 5550 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AscI GG'CGCG_CC Cuts at: 2205 2205 Size: 5578 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2294 2526 2903 1870 Size: 73 204 147 232 377 4545 Fragments arranged by size: 4545 377 232 204 147 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2123 2162 2272 2899 Size: 325 217 627 224 112 39 110 627 Cuts at: 2899 4354 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 627 325 224 217 112 110 39 BanII G_rGCy'C Cuts at: 1391 2049 2117 2145 2897 1391 Size: 658 68 28 752 4072 Fragments arranged by size: 4072 752 658 68 28 BbeI G_GCGC'C Cuts at: 2015 2127 2166 2015 Size: 112 39 5427 Fragments arranged by size: 5427 112 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2121 2160 Size: 107 122 71 631 605 337 112 39 Cuts at: 2160 2591 4189 136 Size: 431 1598 1525 Fragments arranged by size: 1598 1525 631 605 431 337 122 112 107 71 39 BglII A'GATC_T Cuts at: 2535 5573 2535 Size: 3038 2540 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2117 2145 2349 3639 4795 1790 Size: 327 28 204 1290 1156 2573 Fragments arranged by size: 2573 1290 1156 327 204 28 BmgBI CAC'GTC Cuts at: 1318 2517 1318 Size: 1199 4379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2341 4238 1239 Size: 1102 1897 2579 Fragments arranged by size: 2579 1897 1102 Bpu10I CC'TnA_GC Cuts at: 2243 2243 Size: 5578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 4568 4866 3703 Size: 865 298 4415 Fragments arranged by size: 4415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2359 2375 4241 882 Size: 617 860 16 1866 2219 Fragments arranged by size: 2219 1866 860 617 16 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2124 2163 2489 Size: 53 83 186 936 479 112 39 326 Cuts at: 2489 2742 3385 3767 4402 275 Size: 253 643 382 635 1451 Fragments arranged by size: 1451 936 643 635 479 382 326 253 186 112 83 53 39 BsaWI w'CCGG_w Cuts at: 2484 2652 4011 4409 4683 4830 5241 2484 Size: 168 1359 398 274 147 411 2821 Fragments arranged by size: 2821 1359 411 398 274 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5353 Fragments arranged by size: 5353 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2699 2852 1186 Size: 1513 153 3912 Fragments arranged by size: 3912 1513 153 BseYI C'CCAG_C Cuts at: 4781 5277 4781 Size: 496 5082 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 2777 1163 Size: 573 816 225 3964 Fragments arranged by size: 3964 816 573 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2433 3028 3789 4817 1540 Size: 275 186 432 595 761 1028 2301 Fragments arranged by size: 2301 1028 761 595 432 275 186 BsiHKAI G_wGCw'C Cuts at: 3639 3724 4795 3639 Size: 85 1071 4422 Fragments arranged by size: 4422 1071 85 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 BsmBI CGTCTCn'nnnn_ Cuts at: 2181 2181 Size: 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2117 2145 2349 2897 3639 3724 Size: 399 259 68 28 204 548 742 85 Cuts at: 3724 4795 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 548 399 259 204 85 68 28 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 2933 844 Size: 1776 313 3489 Fragments arranged by size: 3489 1776 313 BsrBI CCG'CTC Cuts at: 1394 1823 2689 2701 2785 3465 1394 Size: 429 866 12 84 680 3507 Fragments arranged by size: 3507 866 680 429 84 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4073 4247 65 Size: 1183 2825 174 1396 Fragments arranged by size: 2825 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2275 2715 4222 4409 1700 Size: 110 465 440 1507 187 2869 Fragments arranged by size: 2869 1507 465 440 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 2022 2205 2322 1925 Size: 97 183 117 5181 Fragments arranged by size: 5181 183 117 97 BssSI C'ACGA_G Cuts at: 2042 3638 4650 2042 Size: 1596 1012 2970 Fragments arranged by size: 2970 1596 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2824 794 Size: 2030 3548 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstXI CCAn_nnnn'nTGG Cuts at: 2134 2134 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2070 2225 2535 2908 3275 3660 3677 5129 Size: 98 155 310 373 367 385 17 1452 Cuts at: 5129 5215 5227 5573 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 373 367 346 310 155 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2108 2349 513 Size: 552 939 104 241 3742 Fragments arranged by size: 3742 939 552 241 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2546 487 Size: 683 56 1320 3519 Fragments arranged by size: 3519 1320 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3888 3908 3094 Size: 794 20 4764 Fragments arranged by size: 4764 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2630 4585 809 Size: 507 1314 1955 1802 Fragments arranged by size: 1955 1802 1314 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 2467 3025 Size: 54 1425 50 275 18 600 37 558 Cuts at: 3025 3914 5543 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 600 558 275 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2430 3025 1537 Size: 275 618 595 4090 Fragments arranged by size: 4090 618 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3508 1353 Size: 2155 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2696 2711 4165 4539 4685 387 Size: 1064 138 1107 15 1454 374 146 1280 Fragments arranged by size: 1454 1280 1107 1064 374 146 138 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2341 3641 4238 5025 5293 1239 Size: 393 709 1300 597 787 268 1524 Fragments arranged by size: 1524 1300 787 709 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2113 2114 2141 2142 2113 Size: 1 27 1 5549 Fragments arranged by size: 5549 27 1 1 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2015 2127 2166 2255 2557 2600 4725 1678 Size: 337 112 39 89 302 43 2125 2531 Fragments arranged by size: 2531 2125 337 302 112 89 43 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 4219 4251 4293 Size: 32 135 32 638 32 1977 32 42 Cuts at: 4293 4325 1373 Size: 32 2626 Fragments arranged by size: 2626 1977 638 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KasI G'GCGC_C Cuts at: 2011 2123 2162 2011 Size: 112 39 5427 Fragments arranged by size: 5427 112 39 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4692 4876 1305 Size: 3387 184 2007 Fragments arranged by size: 3387 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3536 3895 4054 518 Size: 648 2370 359 159 2042 Fragments arranged by size: 2370 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2290 2426 2731 2861 2994 3671 4819 Size: 504 180 136 305 130 133 677 1148 Cuts at: 4819 5064 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 504 305 245 180 136 133 130 NaeI GCC'GGC Cuts at: 2277 2717 2277 Size: 440 5138 NarI GG'CG_CC Cuts at: 2012 2124 2163 2012 Size: 112 39 5427 Fragments arranged by size: 5427 112 39 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4087 Fragments arranged by size: 4087 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2275 2715 2275 Size: 440 5138 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 2430 3025 2430 Size: 595 4983 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 2832 1348 Size: 1484 4094 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2113 2141 2113 Size: 28 5550 PstI C_TGCA'G Cuts at: 830 2730 2820 2829 2930 830 Size: 1900 90 9 101 3478 Fragments arranged by size: 3478 1900 101 90 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2731 2731 Size: 5578 SacII CC_GC'GG Cuts at: 2111 2111 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SexAI A'CCwGG_T Cuts at: 2639 2639 Size: 5578 SfcI C'TryA_G Cuts at: 826 1045 2726 2816 2825 2926 4061 4742 4933 Size: 219 1681 90 9 101 1135 681 191 Cuts at: 4933 826 Size: 1471 Fragments arranged by size: 1681 1471 1135 681 219 191 101 90 9 SfoI GGC'GCC Cuts at: 2013 2125 2164 2013 Size: 112 39 5427 Fragments arranged by size: 5427 112 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2149 2296 2528 2905 1872 Size: 277 147 232 377 4545 Fragments arranged by size: 4545 377 277 232 147 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 4583 4845 820 Size: 197 926 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2004 2362 2850 3017 513 Size: 552 939 358 488 167 3074 Fragments arranged by size: 3074 939 552 488 358 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3824 5536 96 Size: 274 80 33 51 1443 1847 1712 138 Fragments arranged by size: 1847 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2147 2294 2526 2903 1870 Size: 277 147 232 377 4545 Fragments arranged by size: 4545 377 277 232 147 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI