################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Cac8I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 457 CGCG CG^CG AocI 489 CCTCAGG CC^T[ACGT]AGG Aor51HI 428 AGCGCT AGC^GCT BalI 437 TGGCCA TGG^CCA BamHI 494 GGATCC G^GATCC BanI 479 GGCGCC G^G[CT][GA]CC BbeI 479 GGCGCC GGCGC^C BbvCI 402 CCTCAGC CCTCAGC(-5/-2) BlpI 956 GCTCAGC GC^T[ACGT]AGC BpmI 301 CTGGAG CTGGAG(16/14) Bpu1102I 956 GCTCAGC GC^T[ACGT]AGC BsaAI 433 TACGTG [CT]AC^GT[GA] BspMI 1074 ACCTGC ACCTGC(4/8) BsrDI 185 GCAATG GCAATG(2/0) BstEII 654 GGTCACC G^GT[ACGT]ACC BstPI 654 GGTCACC G^GT[ACGT]ACC BstUI 457 CGCG CG^CG Bsu36I 489 CCTCAGG CC^T[ACGT]AGG CelII 956 GCTCAGC GC^T[ACGT]AGC DraIII 1108 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 271 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 428 AGCGCT AGC^GCT Eco81I 489 CCTCAGG CC^T[ACGT]AGG EcoO65I 654 GGTCACC G^GT[ACGT]ACC HgaI 231 GACGC GACGC(5/10) HindIII 1224 AAGCTT A^AGCTT KasI 479 GGCGCC G^GCGCC MaeII 434 ACGT A^CGT MaeIII 655 GTCAC ^GT[ACGT]AC MluNI 437 TGGCCA TGG^CCA MscI 437 TGGCCA TGG^CCA MseI 1235 TTAA T^TAA MvnI 457 CGCG CG^CG NarI 479 GGCGCC GG^CGCC NcoI 552 CCATGG C^CATGG PflMI 1107 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 863 GACTC GAGTC(4/5) PvuII 959 CAGCTG CAG^CTG SacI 954 GAGCTC GAGCT^C SapI 525 GCTCTTC GCTCTTC(1/4) SfoI 479 GGCGCC GGC^GCC SmlI 745 CTTGAG C^T[CT][GA]AG SspI 769 AATATT AAT^ATT Tru9I 1235 TTAA T^TAA Tsp45I 655 GTCAC ^GT[GC]AC Van91I 1107 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 231 GACGCC G[GA]^CG[CT]C 479 GGCGCC Asp700I 13 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1086 GAACATCTTC BbsI 228 GAAGAC GAAGAC(2/6) 940 GTCTTC BcnI 476 CCCGG CC^[GC]GG 483 CCGGG BfaI 52 CTAG C^TAG 1193 CTAG BpuAI 228 GAAGAC GAAGAC(2/6) 940 GTCTTC BsaHI 231 GACGCC G[GA]^CG[CT]C 479 GGCGCC BsaI 379 GAGACC GGTCTC(1/5) 264 GGTCTC DsaI 347 CCGTGG C^C[GA][CT]GG 552 CCATGG EaeI 437 TGGCCA [CT]^GGCC[GA] 602 CGGCCA EcoT14I 288 CCTTGG C^C[AT][AT]GG 552 CCATGG HaeII 428 AGCGCT [GA]GCGC^[CT] 479 GGCGCC HapII 477 CCGG C^CGG 483 CCGG Hin1I 231 GACGCC G[GA]^CG[CT]C 479 GGCGCC HincII 796 GTCAAC GT[CT]^[GA]AC 964 GTCAAC HindII 796 GTCAAC GT[CT]^[GA]AC 964 GTCAAC HpaII 477 CCGG C^CGG 483 CCGG MaeI 52 CTAG C^TAG 1193 CTAG MspA1I 959 CAGCTG C[AC]G^C[GT]G 1081 CAGCGG MspI 477 CCGG C^CGG 483 CCGG NciI 476 CCCGG CC^[GC]GG 483 CCGGG PpuMI 583 GGGTCCC [GA]G^G[AT]CC[CT] 1134 AGGTCCC StyI 288 CCTTGG C^C[AT][AT]GG 552 CCATGG TfiI 207 GATTC G^A[AT]TC 895 GAATC TspRI 696 CAGTG CA[GC]TG[ACGT][ACGT]^ 1042 CAGTG XcmI 413 CCACCGTCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 578 CCAATGGGTCCCTGG XmnI 13 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1086 GAACATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI ApaI AspHI AvaII BanII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp509I XhoII