(Circular) (Six-base) MAPSORT of: pFN21AE9530.seq Check: 6976 from: 1 to: 5950 pFN21AE9530 5950 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3088 3088 Size: 5950 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2009 2242 2860 4013 5397 5665 1632 Size: 377 233 618 1153 1384 268 1917 Fragments arranged by size: 1917 1384 1153 618 377 268 233 AfeI AGC'GCT Cuts at: 2435 2435 Size: 5950 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaI G_GGCC'C Cuts at: 2352 2494 2851 2352 Size: 142 357 5451 Fragments arranged by size: 5451 357 142 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 2499 3280 3647 2499 Size: 781 367 4802 Fragments arranged by size: 4802 781 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2484 3271 4726 618 Size: 325 217 627 697 787 1455 1842 Fragments arranged by size: 1842 1455 787 697 627 325 217 BanII G_rGCy'C Cuts at: 1391 2352 2494 2851 2963 3269 1391 Size: 961 142 357 112 306 4072 Fragments arranged by size: 4072 961 357 306 142 112 BbeI G_GCGC'C Cuts at: 2488 2488 Size: 5950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2240 2938 928 Size: 971 341 698 3940 Fragments arranged by size: 3940 971 698 341 BbvCI CC'TCA_GC Cuts at: 2408 2408 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2962 3367 2962 Size: 405 5545 Bme1580I G_kGCm'C Cuts at: 1790 2352 2494 2851 4011 5167 1790 Size: 562 142 357 1160 1156 2573 Fragments arranged by size: 2573 1160 1156 562 357 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 4610 1239 Size: 1088 2283 2579 Fragments arranged by size: 2579 2283 1088 Bpu10I CC'TnA_GC Cuts at: 2408 2957 2408 Size: 549 5401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2771 4075 4940 5238 2771 Size: 1304 865 298 3483 Fragments arranged by size: 3483 1304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2275 2378 4613 882 Size: 617 776 103 2235 2219 Fragments arranged by size: 2235 2219 776 617 103 BsaAI yAC'GTr Cuts at: 493 1204 2440 3741 493 Size: 711 1236 1301 2702 Fragments arranged by size: 2702 1301 1236 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2237 2485 3757 4139 Size: 53 83 186 936 704 248 1272 382 Cuts at: 4139 4774 275 Size: 635 1451 Fragments arranged by size: 1451 1272 936 704 635 382 248 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2672 2903 1186 Size: 922 564 231 4233 Fragments arranged by size: 4233 922 564 231 BseYI C'CCAG_C Cuts at: 3057 3084 5153 5649 3057 Size: 27 2069 496 3358 Fragments arranged by size: 3358 2069 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2480 2532 2963 4011 4096 5167 2480 Size: 52 431 1048 85 1071 3263 Fragments arranged by size: 3263 1071 1048 431 85 52 BsmI GAATG_Cn' Cuts at: 2035 2304 2773 3014 3113 3461 3554 2035 Size: 269 469 241 99 348 93 4431 Fragments arranged by size: 4431 469 348 269 241 99 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2480 2494 2532 2851 2963 3269 Size: 399 562 128 14 38 319 112 306 Cuts at: 3269 4011 4096 5167 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 399 319 306 128 112 85 38 14 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3088 3305 844 Size: 2244 217 3489 Fragments arranged by size: 3489 2244 217 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2197 4445 4619 65 Size: 1183 949 2248 174 1396 Fragments arranged by size: 2248 1396 1183 949 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 2659 1184 Size: 1475 4475 BstYI r'GATC_y Cuts at: 1972 2040 2062 2499 2637 3280 3647 4032 4049 Size: 68 22 437 138 643 367 385 17 Cuts at: 4049 5501 5587 5599 5945 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 643 437 385 367 346 138 86 68 22 17 12 Bsu36I CC'TnA_GG Cuts at: 2495 2495 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2352 2557 513 Size: 552 1287 205 3906 Fragments arranged by size: 3906 1287 552 205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2154 2189 529 Size: 35 1590 35 4290 Fragments arranged by size: 4290 1590 35 35 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DraIII CAC_nnn'GTG Cuts at: 3118 3118 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2282 4957 809 Size: 507 966 2675 1802 Fragments arranged by size: 2675 1802 966 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2607 3397 Size: 54 1425 50 275 18 612 165 790 Cuts at: 3397 4286 5915 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 790 612 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 2537 2876 3037 3880 1353 Size: 908 276 339 161 843 3423 Fragments arranged by size: 3423 908 843 339 276 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2961 2961 Size: 5950 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2009 2242 2327 2860 4013 4610 5397 Size: 393 377 233 85 533 1153 597 787 Cuts at: 5397 5665 1239 Size: 268 1524 Fragments arranged by size: 1524 1153 787 597 533 393 377 268 233 85 EcoO109I rG'GnC_Cy Cuts at: 2491 2589 2848 3140 2491 Size: 98 259 292 5301 Fragments arranged by size: 5301 292 259 98 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2437 2488 5097 1678 Size: 759 51 2609 2531 Fragments arranged by size: 2609 2531 759 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373 Size: 32 135 32 3019 32 42 32 2626 Fragments arranged by size: 3019 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2803 2971 3294 3540 669 Size: 988 281 865 168 323 246 3079 Fragments arranged by size: 3079 988 865 323 281 246 168 HindIII A'AGCT_T Cuts at: 748 3229 748 Size: 2481 3469 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KasI G'GCGC_C Cuts at: 2484 2484 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 10 Size: 54 1425 343 612 3516 Fragments arranged by size: 3516 1425 612 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3908 4267 4426 518 Size: 648 2742 359 159 2042 Fragments arranged by size: 2742 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2966 3088 3366 4043 5191 5436 1606 Size: 1360 122 278 677 1148 245 2120 Fragments arranged by size: 2120 1360 1148 677 278 245 122 NarI GG'CG_CC Cuts at: 2485 2485 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 2557 513 Size: 552 1492 3906 Fragments arranged by size: 3906 1492 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 3308 4853 1132 Size: 97 2079 1545 2229 Fragments arranged by size: 2229 2079 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 1108 Size: 2010 3940 PfoI T'CCnGG_A Cuts at: 2036 2036 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2589 3140 2589 Size: 551 5399 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2348 2490 2847 2348 Size: 142 357 5451 Fragments arranged by size: 5451 357 142 PstI C_TGCA'G Cuts at: 830 2220 2475 2792 3302 830 Size: 1390 255 317 510 3478 Fragments arranged by size: 3478 1390 510 317 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2966 2966 Size: 5950 SacI G_AGCT'C Cuts at: 2963 2963 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SanDI GG'GwC_CC Cuts at: 2589 2589 Size: 5950 SapI GCTCTTCn'nnn_ Cuts at: 2537 2537 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2216 2471 2788 3298 4433 5114 5305 Size: 219 1171 255 317 510 1135 681 191 Cuts at: 5305 826 Size: 1471 Fragments arranged by size: 1471 1171 1135 681 510 317 255 219 191 SfoI GGC'GCC Cuts at: 2486 2486 Size: 5950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 4054 4955 5217 820 Size: 197 926 807 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 807 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 2776 3874 5 Size: 47 2724 1098 2081 Fragments arranged by size: 2724 2081 1098 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2293 2557 3389 513 Size: 552 1228 264 832 3074 Fragments arranged by size: 3074 1228 832 552 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2880 3143 3977 3994 4147 4332 4777 Size: 1255 1300 263 834 17 153 185 445 Cuts at: 4777 325 Size: 1498 Fragments arranged by size: 1498 1300 1255 834 445 263 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2425 2590 2011 Size: 414 165 5371 Fragments arranged by size: 5371 414 165 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2022 3095 4079 2022 Size: 1073 984 3893 Fragments arranged by size: 3893 1073 984 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI