(Linear) (Six-base) MAPSORT of: KIEE9530.seq Check: 4577 from: 1 to: 1245 KIEE9530.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1083 1245 Size: 1083 162 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 237 855 1245 Size: 4 233 618 390 Fragments arranged by size: 618 390 233 4 AfeI AGC'GCT Cuts at: 0 430 1245 Size: 430 815 ApaI G_GGCC'C Cuts at: 0 347 489 846 1245 Size: 347 142 357 399 Fragments arranged by size: 399 357 347 142 BamHI G'GATC_C Cuts at: 0 494 1245 Size: 494 751 BanI G'GyrC_C Cuts at: 0 479 1245 Size: 479 766 BanII G_rGCy'C Cuts at: 0 347 489 846 958 1245 Size: 347 142 357 112 287 Fragments arranged by size: 357 347 287 142 112 BbeI G_GCGC'C Cuts at: 0 483 1245 Size: 483 762 BbsI GAAGACnn'nnnn_ Cuts at: 0 235 933 1245 Size: 235 698 312 Fragments arranged by size: 698 312 235 BbvCI CC'TCA_GC Cuts at: 0 403 1245 Size: 403 842 BlpI GC'TnA_GC Cuts at: 0 957 1245 Size: 957 288 Bme1580I G_kGCm'C Cuts at: 0 347 489 846 1245 Size: 347 142 357 399 Fragments arranged by size: 399 357 347 142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1245 Size: 322 923 Bpu10I CC'TnA_GC Cuts at: 0 403 952 1245 Size: 403 549 293 Fragments arranged by size: 549 403 293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 1245 Size: 766 479 BsaI GGTCTCn'nnnn_ Cuts at: 0 270 373 1245 Size: 270 103 872 Fragments arranged by size: 872 270 103 BsaAI yAC'GTr Cuts at: 0 435 1245 Size: 435 810 BsaHI Gr'CG_yC Cuts at: 0 232 480 1245 Size: 232 248 765 Fragments arranged by size: 765 248 232 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 667 898 1245 Size: 103 564 231 347 Fragments arranged by size: 564 347 231 103 BseYI C'CCAG_C Cuts at: 0 1052 1079 1245 Size: 1052 27 166 Fragments arranged by size: 1052 166 27 BsiHKAI G_wGCw'C Cuts at: 0 475 527 958 1245 Size: 475 52 431 287 Fragments arranged by size: 475 431 287 52 BsmI GAATG_Cn' Cuts at: 0 30 299 768 1009 1108 1245 Size: 30 269 469 241 99 137 Fragments arranged by size: 469 269 241 137 99 30 Bsp1286I G_dGCh'C Cuts at: 0 347 475 489 527 846 958 1245 Size: 347 128 14 38 319 112 287 Fragments arranged by size: 347 319 287 128 112 38 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1083 1245 Size: 1083 162 BsrDI GCAATG_nn' Cuts at: 0 192 1245 Size: 192 1053 BstEII G'GTnAC_C Cuts at: 0 654 1245 Size: 654 591 BstYI r'GATC_y Cuts at: 0 35 57 494 632 1245 Size: 35 22 437 138 613 Fragments arranged by size: 613 437 138 35 22 Bsu36I CC'TnA_GG Cuts at: 0 490 1245 Size: 490 755 BtgI C'CryG_G Cuts at: 0 347 552 1245 Size: 347 205 693 Fragments arranged by size: 693 347 205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 149 184 1245 Size: 149 35 1061 Fragments arranged by size: 1061 149 35 DraIII CAC_nnn'GTG Cuts at: 0 1113 1245 Size: 1113 132 DrdI GACnn_nn'nnGTC Cuts at: 0 277 1245 Size: 277 968 EaeI y'GGCC_r Cuts at: 0 437 602 1245 Size: 437 165 643 Fragments arranged by size: 643 437 165 EarI CTCTTCn'nnn_ Cuts at: 0 256 532 871 1032 1245 Size: 256 276 339 161 213 Fragments arranged by size: 339 276 256 213 161 EcoICRI GAG'CTC Cuts at: 0 956 1245 Size: 956 289 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 237 322 855 1245 Size: 4 233 85 533 390 Fragments arranged by size: 533 390 233 85 4 EcoO109I rG'GnC_Cy Cuts at: 0 486 584 843 1135 1245 Size: 486 98 259 292 110 Fragments arranged by size: 486 292 259 110 98 HaeII r_GCGC'y Cuts at: 0 432 483 1245 Size: 432 51 762 Fragments arranged by size: 762 432 51 HincII GTy'rAC Cuts at: 0 798 966 1245 Size: 798 168 279 Fragments arranged by size: 798 279 168 HindIII A'AGCT_T Cuts at: 0 1224 1245 Size: 1224 21 KasI G'GCGC_C Cuts at: 0 479 1245 Size: 479 766 MscI TGG'CCA Cuts at: 0 439 1245 Size: 439 806 MspA1I CmG'CkG Cuts at: 0 961 1083 1245 Size: 961 122 162 Fragments arranged by size: 961 162 122 NarI GG'CG_CC Cuts at: 0 480 1245 Size: 480 765 NcoI C'CATG_G Cuts at: 0 552 1245 Size: 552 693 PflMI CCAn_nnn'nTGG Cuts at: 0 1113 1245 Size: 1113 132 PfoI T'CCnGG_A Cuts at: 0 31 1245 Size: 31 1214 PpuMI rG'GwC_Cy Cuts at: 0 584 1135 1245 Size: 584 551 110 Fragments arranged by size: 584 551 110 PspOMI G'GGCC_C Cuts at: 0 343 485 842 1245 Size: 343 142 357 403 Fragments arranged by size: 403 357 343 142 PstI C_TGCA'G Cuts at: 0 215 470 787 1245 Size: 215 255 317 458 Fragments arranged by size: 458 317 255 215 PvuII CAG'CTG Cuts at: 0 961 1245 Size: 961 284 SacI G_AGCT'C Cuts at: 0 958 1245 Size: 958 287 SanDI GG'GwC_CC Cuts at: 0 584 1245 Size: 584 661 SapI GCTCTTCn'nnn_ Cuts at: 0 532 1245 Size: 532 713 SfcI C'TryA_G Cuts at: 0 211 466 783 1245 Size: 211 255 317 462 Fragments arranged by size: 462 317 255 211 SfoI GGC'GCC Cuts at: 0 481 1245 Size: 481 764 SmlI C'TyrA_G Cuts at: 0 745 1245 Size: 745 500 SspI AAT'ATT Cuts at: 0 771 1245 Size: 771 474 StyI C'CwwG_G Cuts at: 0 288 552 1245 Size: 288 264 693 Fragments arranged by size: 693 288 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 875 1138 1245 Size: 875 263 107 Fragments arranged by size: 875 263 107 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 420 585 1245 Size: 420 165 660 Fragments arranged by size: 660 420 165 XmnI GAAnn'nnTTC Cuts at: 0 17 1090 1245 Size: 17 1073 155 Fragments arranged by size: 1073 155 17 Enzymes that do cut: AarI AcuI AfeI ApaI BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstEII BstYI Bsu36I BtgI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HincII HindIII KasI MscI MspA1I NarI NcoI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI SfoI SmlI SspI StyI TaqII XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HpaI KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI