################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflIII 274 ACACGT A^C[GA][CT]GT AluI 686 AGCT AG^CT AlwI 435 GGATC GGATC(4/5) AlwNI 596 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 219 CCTCAGG CC^T[ACGT]AGG AseI 148 ATTAAT AT^TAAT AsnI 148 ATTAAT AT^TAAT AvaI 710 CCCGGG C^[CT]CG[GA]G AvaII 394 GGACC G^G[AT]CC BbrPI 275 CACGTG CAC^GTG BbvI 211 GCAGC GCAGC(8/12) BsaAI 275 CACGTG [CT]AC^GT[GA] BsmAI 517 GAGAC GTCTC(1/5) BsoBI 710 CCCGGG C^[CT]CG[GA]G Bsu36I 219 CCTCAGG CC^T[ACGT]AGG Cac8I 484 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 394 GGACC G^G[ACGT]CC DrdI 183 GACATCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 219 CCTCAGG CC^T[ACGT]AGG EcoT14I 360 CCATGG C^C[AT][AT]GG EcoT22I 584 ATGCAT ATGCA^T Fnu4HI 211 GCAGC GC^[ACGT]GC HapII 711 CCGG C^CGG HgaI 757 GACGC GACGC(5/10) HindIII 685 AAGCTT A^AGCTT HpaI 715 GTTAAC GTT^AAC HpaII 711 CCGG C^CGG HphI 364 GGTGA GGTGA(8/7) ItaI 211 GCAGC GC^[ACGT]GC MaeII 276 ACGT A^CGT MslI 174 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 711 CCGG C^CGG MwoI 326 GCAAAAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 360 CCATGG C^CATGG NsiI 584 ATGCAT ATGCA^T PmaCI 275 CACGTG CAC^GTG PmlI 275 CACGTG CAC^GTG PshBI 148 ATTAAT AT^TAAT Sau96I 394 GGACC G^G[ACGT]CC SexAI 600 ACCTGGT A^CC[AT]GGT SmaI 710 CCCGGG CCC^GGG StuI 482 AGGCCT AGG^CCT StyI 360 CCATGG C^C[AT][AT]GG TaqI 206 TCGA T^CGA TseI 211 GCAGC G^C[AT]GC TthHB8I 206 TCGA T^CGA XcmI 35 CCATTAAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 710 CCCGGG C^CCGGG XmaI 710 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 197 GTAC GT^AC 494 GTAC BcnI 710 CCCGG CC^[GC]GG 711 CCGGG BsiYI 124 CCTGAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 720 CCGAAAGGTGG BslI 124 CCTGAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 720 CCGAAAGGTGG BstF5I 645 CATCC GGATG(2/0) 630 GGATG DsaI 360 CCATGG C^C[GA][CT]GG 489 CCGTGG FokI 645 CATCC GGATG(9/13) 630 GGATG HaeIII 483 GGCC GG^CC 570 GGCC HincII 400 GTTGAC GT[CT]^[GA]AC 715 GTTAAC HindII 400 GTTGAC GT[CT]^[GA]AC 715 GTTAAC NciI 710 CCCGG CC^[GC]GG 711 CCGGG NlaIV 48 GGAACC GG[ACGT]^[ACGT]CC 634 GGCTCC PleI 82 GACTC GAGTC(4/5) 742 GAGTC RsaI 197 GTAC GT^AC 494 GTAC SfaNI 208 GATGC GCATC(5/9) 297 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AcsI ApoI BsaJI BsrI BssKI BstNI DdeI DpnII EcoRII HinfI MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI