(Circular) (Six-base) MAPSORT of: pFN21AB9950.seq Check: 8619 from: 1 to: 6730 pFN21AB9950 6730 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2358 2358 Size: 6730 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4540 278 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4073 1937 Size: 2136 4594 Acc65I G'GTAC_C Cuts at: 4051 4051 Size: 6730 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2537 2880 3893 4793 6177 6445 1632 Size: 905 343 1013 900 1384 268 1917 Fragments arranged by size: 1917 1384 1013 905 900 343 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6533 AflIII A'CryG_T Cuts at: 1203 5629 6606 1203 Size: 4426 977 1327 Fragments arranged by size: 4426 1327 977 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 3211 5459 3211 Size: 2248 4482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6730 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3081 1184 Size: 1897 4833 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 3709 3741 2375 Size: 32 1302 32 5364 Fragments arranged by size: 5364 1302 32 32 AlwNI CAG_nnn'CTG Cuts at: 2537 3948 4473 6045 2537 Size: 1411 525 1572 3222 Fragments arranged by size: 3222 1572 1411 525 ApaLI G'TGCA_C Cuts at: 4787 5943 4787 Size: 1156 5574 ApoI r'AATT_y Cuts at: 1493 2837 2971 3371 3425 4039 4265 4417 6526 Size: 1344 134 400 54 614 226 152 2109 Cuts at: 6526 1493 Size: 1697 Fragments arranged by size: 2109 1697 1344 614 400 226 152 134 54 AseI AT'TA_AT Cuts at: 160 1032 5284 160 Size: 872 4252 1606 Fragments arranged by size: 4252 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 2207 2526 4055 1870 Size: 73 264 319 1529 4545 Fragments arranged by size: 4545 1529 319 264 73 BamHI G'GATC_C Cuts at: 2162 3801 4060 4427 2162 Size: 1639 259 367 4465 Fragments arranged by size: 4465 1639 367 259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4051 5506 618 Size: 325 217 627 2264 1455 1842 Fragments arranged by size: 2264 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3356 4049 1391 Size: 1965 693 4072 Fragments arranged by size: 4072 1965 693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2724 3987 928 Size: 971 825 1263 3671 Fragments arranged by size: 3671 1263 971 825 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4921 4955 1903 Size: 34 2984 34 3678 Fragments arranged by size: 3678 2984 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 4622 5832 2002 Size: 2620 1210 2900 Fragments arranged by size: 2900 2620 1210 BclI T'GATC_A Cuts at: 1548 3156 3342 1548 Size: 1608 186 4936 Fragments arranged by size: 4936 1608 186 BfrBI ATG'CAT Cuts at: 2282 2282 Size: 6730 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5341 136 Size: 107 122 71 631 605 3669 1525 Fragments arranged by size: 3669 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3643 6725 3643 Size: 3082 3648 BlpI GC'TnA_GC Cuts at: 4147 4147 Size: 6730 Bme1580I G_kGCm'C Cuts at: 1790 4791 5947 1790 Size: 3001 1156 2573 Fragments arranged by size: 3001 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6730 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4461 5419 442 Size: 416 53 83 399 24 3044 958 1753 Fragments arranged by size: 3044 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2893 3729 5390 1239 Size: 1654 836 1661 2579 Fragments arranged by size: 2579 1661 1654 836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4855 5720 6018 4855 Size: 865 298 5567 Fragments arranged by size: 5567 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3883 5393 882 Size: 617 2384 1510 2219 Fragments arranged by size: 2384 2219 1510 617 BsaAI yAC'GTr Cuts at: 493 1204 3055 4521 493 Size: 711 1851 1466 2702 Fragments arranged by size: 2702 1851 1466 711 BsaBI GATnn'nnATC Cuts at: 2917 6724 2917 Size: 3807 2923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2219 4537 4919 5554 Size: 53 83 186 936 686 2318 382 635 Cuts at: 5554 275 Size: 1451 Fragments arranged by size: 2318 1451 936 686 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5163 5561 5835 5982 6393 5163 Size: 398 274 147 411 5500 Fragments arranged by size: 5500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2627 1178 Size: 30 165 30 1194 30 5281 Fragments arranged by size: 5281 1194 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 3523 1186 Size: 1039 1298 4393 Fragments arranged by size: 4393 1298 1039 BseYI C'CCAG_C Cuts at: 2616 2682 3271 3301 3537 5933 6429 2616 Size: 66 589 30 236 2396 496 2917 Fragments arranged by size: 2917 2396 589 496 236 66 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4180 4941 5969 1540 Size: 275 186 2179 761 1028 2301 Fragments arranged by size: 2301 2179 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4791 4876 5947 4791 Size: 85 1071 5574 Fragments arranged by size: 5574 1071 85 BsmI GAATG_Cn' Cuts at: 4241 4334 4241 Size: 93 6637 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3356 4049 4791 4876 5947 1391 Size: 399 1566 693 742 85 1071 2174 Fragments arranged by size: 2174 1566 1071 742 693 399 85 BspEI T'CCGG_A Cuts at: 6393 6393 Size: 6730 BspHI T'CATG_A Cuts at: 4619 6349 4619 Size: 1730 5000 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 4085 844 Size: 1514 1727 3489 Fragments arranged by size: 3489 1727 1514 BsrBI CCG'CTC Cuts at: 1394 1823 4617 1394 Size: 429 2794 3507 Fragments arranged by size: 3507 2794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3490 5225 5399 65 Size: 1183 2242 1735 174 1396 Fragments arranged by size: 2242 1735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3900 5374 5561 1700 Size: 110 2090 1474 187 2869 Fragments arranged by size: 2869 2090 1474 187 110 BsrGI T'GTAC_A Cuts at: 96 2991 3939 96 Size: 2895 948 2887 Fragments arranged by size: 2895 2887 948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6730 BssSI C'ACGA_G Cuts at: 3953 4790 5802 3953 Size: 837 1012 4881 Fragments arranged by size: 4881 1012 837 BstAPI GCAn_nnn'nTGC Cuts at: 794 3489 794 Size: 2695 4035 BstBI TT'CG_AA Cuts at: 5534 5534 Size: 6730 BstEII G'GTnAC_C Cuts at: 1184 2339 1184 Size: 1155 5575 BstXI CCAn_nnnn'nTGG Cuts at: 3735 3735 Size: 6730 BstYI r'GATC_y Cuts at: 1972 2162 3643 3801 4060 4427 4812 4829 6281 Size: 190 1481 158 259 367 385 17 1452 Cuts at: 6281 6367 6379 6725 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1481 1452 385 367 346 259 190 158 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3506 3506 Size: 6730 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5239 Fragments arranged by size: 5239 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5991 Fragments arranged by size: 5991 683 56 BtsI GCAGTG_nn' Cuts at: 2773 4246 5040 5060 2773 Size: 1473 794 20 4443 Fragments arranged by size: 4443 1473 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6730 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6695 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DraIII CAC_nnn'GTG Cuts at: 3178 3178 Size: 6730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3701 5737 809 Size: 507 2385 2036 1802 Fragments arranged by size: 2385 2036 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3280 4177 5066 Size: 54 1425 50 275 18 1450 897 889 Cuts at: 5066 6695 8 Size: 1629 43 Fragments arranged by size: 1629 1450 1425 897 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4177 1537 Size: 275 2365 4090 Fragments arranged by size: 4090 2365 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2472 2859 3243 4660 1353 Size: 933 186 387 384 1417 3423 Fragments arranged by size: 3423 1417 933 387 384 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3158 5317 5691 5837 387 Size: 1064 138 1569 2159 374 146 1280 Fragments arranged by size: 2159 1569 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2880 2893 3729 3893 4793 5390 Size: 393 905 343 13 836 164 900 597 Cuts at: 5390 6177 6445 1239 Size: 787 268 1524 Fragments arranged by size: 1524 905 900 836 787 597 393 343 268 164 13 EcoO109I rG'GnC_Cy Cuts at: 3199 3199 Size: 6730 EcoRI G'AATT_C Cuts at: 2837 3371 4039 4417 2837 Size: 534 668 378 5150 Fragments arranged by size: 5150 668 534 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2051 2108 2282 2051 Size: 57 174 6499 Fragments arranged by size: 6499 174 57 FspI TGC'GCA Cuts at: 5236 5236 Size: 6730 HaeII r_GCGC'y Cuts at: 1678 5877 1678 Size: 4199 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2287 2319 2421 Size: 32 135 32 428 32 255 32 102 Cuts at: 2421 2453 2543 2575 2579 2611 2645 2677 3197 Size: 32 90 32 4 32 34 32 520 Cuts at: 3197 3229 5371 5403 5445 5477 1373 Size: 32 2142 32 42 32 2626 Fragments arranged by size: 2626 2142 520 428 255 135 102 90 42 34 32 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3183 4074 4320 669 Size: 988 281 1245 891 246 3079 Fragments arranged by size: 3079 1245 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 3119 748 Size: 2371 4359 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KpnI G_GTAC'C Cuts at: 4055 4055 Size: 6730 MfeI C'AATT_G Cuts at: 4329 4329 Size: 6730 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3694 5844 6028 1305 Size: 2389 2150 184 2007 Fragments arranged by size: 2389 2150 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3282 10 Size: 54 1425 343 1450 3458 Fragments arranged by size: 3458 1450 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3063 4688 5047 5206 518 Size: 648 1897 1625 359 159 2042 Fragments arranged by size: 2042 1897 1625 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3301 3945 4146 4823 5971 6216 1606 Size: 1695 644 201 677 1148 245 2120 Fragments arranged by size: 2120 1695 1148 677 644 245 201 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5239 Fragments arranged by size: 5239 939 552 NdeI CA'TA_TG Cuts at: 387 2449 387 Size: 2062 4668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 4177 4177 Size: 6730 NsiI A_TGCA'T Cuts at: 2284 2284 Size: 6730 NspI r_CATG'y Cuts at: 1132 1229 2701 4031 4088 5633 1132 Size: 97 1472 1330 57 1545 2229 Fragments arranged by size: 2229 1545 1472 1330 97 57 PasI CC'CwG_GG Cuts at: 1348 3217 3883 1348 Size: 1869 666 4195 Fragments arranged by size: 4195 1869 666 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 5485 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PmlI CAC'GTG Cuts at: 3055 3055 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2022 2054 2916 2948 4799 4831 6312 6344 2022 Size: 32 862 32 1851 32 1481 32 2408 Fragments arranged by size: 2408 1851 1481 862 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3199 3199 Size: 6730 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6730 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3065 3097 3065 Size: 32 6698 PstI C_TGCA'G Cuts at: 830 2470 4082 830 Size: 1640 1612 3478 Fragments arranged by size: 3478 1640 1612 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 3301 3945 3301 Size: 644 6086 SalI G'TCGA_C Cuts at: 1936 4072 1936 Size: 2136 4594 SbfI CC_TGCA'GG Cuts at: 4082 4082 Size: 6730 ScaI AGT'ACT Cuts at: 4978 6690 4978 Size: 1712 5018 SfcI C'TryA_G Cuts at: 826 1045 2223 2466 4078 5213 5894 6085 826 Size: 219 1178 243 1612 1135 681 191 1471 Fragments arranged by size: 1612 1471 1178 1135 681 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 4057 1872 Size: 2185 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 4834 5735 5997 820 Size: 197 926 264 2627 901 262 1553 Fragments arranged by size: 2627 1553 926 901 264 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 4088 1132 Size: 2956 3774 SspI AAT'ATT Cuts at: 5 52 4654 5 Size: 47 4602 2081 Fragments arranged by size: 4602 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6730 StyI C'CwwG_G Cuts at: 513 1065 2004 2711 4169 513 Size: 552 939 707 1458 3074 Fragments arranged by size: 3074 1458 939 707 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4757 4774 4927 5112 5557 325 Size: 1255 3177 17 153 185 445 1498 Fragments arranged by size: 3177 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2991 3939 4976 Size: 274 80 33 51 1443 1014 948 1037 Cuts at: 4976 6688 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1037 1014 948 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6690 6722 6690 Size: 32 6698 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6730 XbaI T'CTAG_A Cuts at: 2023 4066 2023 Size: 2043 4687 XhoI C'TCGA_G Cuts at: 1943 2207 1943 Size: 264 6466 XmaI C'CCGG_G Cuts at: 1870 4055 1870 Size: 2185 4545 XmnI GAAnn'nnTTC Cuts at: 2031 2034 3375 4008 4859 2031 Size: 3 1341 633 851 3902 Fragments arranged by size: 3902 1341 851 633 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4538 276 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI