################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BciVI BfrI BglI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 1945 GACGTC GACGT^C AccII 2449 CGCG CG^CG AfaI 759 GTAC GT^AC AhdI 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2452 GTGCAC G^TGCAC AlwNI 898 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 2452 GTGCAC G^TGCAC AspEI 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 111 CTCGGG C^[CT]CG[GA]G BalI 500 TGGCCA TGG^CCA BbeI 1931 GGCGCC GGCGC^C BbrPI 2245 CACGTG CAC^GTG BbvCI 2440 GCTGAGG CCTCAGC(-5/-2) BcgI 1523 GCATCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1568 TGATCA T^GATCA BglII 2642 AGATCT A^GATCT BsaI 2578 GAGACC GGTCTC(1/5) BsaWI 1424 ACCGGA [AT]^CCGG[AT] BsgI 2248 GTGCAG GTGCAG(16/14) BsiEI 735 CGATCG CG[GA][CT]^CG BsmBI 2852 CGTCTC CGTCTC(1/5) BsmI 2483 GCATTC GAATGC(1/-1) BsoBI 111 CTCGGG C^[CT]CG[GA]G BspDI 2263 ATCGAT AT^CGAT BspHI 258 TCATGA T^CATGA BspMI 893 ACCTGC ACCTGC(4/8) BstUI 2449 CGCG CG^CG BstXI 2471 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2642 AGATCT [GA]^GATC[CT] ClaI 2263 ATCGAT AT^CGAT Eam1105I 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1002 ATGCAT ATGCA^T FbaI 1568 TGATCA T^GATCA FseI 957 GGCCGGCC GGCCGG^CC HaeII 1931 GGCGCC [GA]GCGC^[CT] HgaI 2215 GACGC GACGC(5/10) HincII 889 GTCAAC GT[CT]^[GA]AC HindII 889 GTCAAC GT[CT]^[GA]AC KasI 1931 GGCGCC G^GCGCC MflI 2642 AGATCT [GA]^GATC[CT] MluNI 500 TGGCCA TGG^CCA MscI 500 TGGCCA TGG^CCA MslI 774 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 2449 CGCG CG^CG NaeI 958 GCCGGC GCC^GGC NarI 1931 GGCGCC GG^CGCC NcoI 198 CCATGG C^CATGG NgoMIV 958 GCCGGC G^CCGGC NsiI 1002 ATGCAT ATGCA^T PmaCI 2245 CACGTG CAC^GTG PmlI 2245 CACGTG CAC^GTG PvuI 735 CGATCG CGAT^CG RcaI 258 TCATGA T^CATGA RsaI 759 GTAC GT^AC SexAI 2390 ACCTGGT A^CC[AT]GGT SfoI 1931 GGCGCC GGC^GCC StuI 1484 AGGCCT AGG^CCT XhoII 2642 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 165 AAATTT [GA]^AATT[CT] 2824 GAATTT AcyI 1931 GGCGCC G[GA]^CG[CT]C 1945 GACGTC ApoI 165 AAATTT [GA]^AATT[CT] 2824 GAATTT Asp700I 2726 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2747 GAAGTAGTTC BcnI 1754 CCCGG CC^[GC]GG 2567 CCGGG BfaI 107 CTAG C^TAG 626 CTAG BlpI 2284 GCTGAGC GC^T[ACGT]AGC 2404 GCTGAGC Bpu1102I 2284 GCTGAGC GC^T[ACGT]AGC 2404 GCTGAGC BsaAI 338 CACGTA [CT]AC^GT[GA] 2245 CACGTG BsaHI 1931 GGCGCC G[GA]^CG[CT]C 1945 GACGTC BsrBI 1438 CCGCTC CCGCTC(-3/-3) 2287 GAGCGG BsrDI 123 CATTGC GCAATG(2/0) 774 CATTGC BsrFI 958 GCCGGC [GA]^CCGG[CT] 1698 GCCGGT BssSI 1294 CTCGTG CACGAG(-5/-1) 2494 CTCGTG CelII 2284 GCTGAGC GC^T[ACGT]AGC 2404 GCTGAGC Cfr10I 958 GCCGGC [GA]^CCGG[CT] 1698 GCCGGT DraI 1069 TTTAAA TTT^AAA 1627 TTTAAA EcoNI 1905 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2616 CCTCTACAAGG Hin1I 1931 GGCGCC G[GA]^CG[CT]C 1945 GACGTC MaeI 107 CTAG C^TAG 626 CTAG NciI 1754 CCCGG CC^[GC]GG 2567 CCGGG NspI 1004 GCATGC [GA]CATG^[CT] 2328 GCATGC PstI 895 CTGCAG CTGCA^G 2036 CTGCAG SacI 1765 GAGCTC GAGCT^C 2692 GAGCTC SphI 1004 GCATGC GCATG^C 2328 GCATGC Tsp45I 930 GTCAC ^GT[GC]AC 1537 GTGAC XcmI 744 CCAGGTGTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2018 CCAGAGCTGCCCTGG XmnI 2726 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2747 GAAGTAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BanI BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I