(Circular) (Six-base) MAPSORT of: pFN21AE9674.seq Check: 4080 from: 1 to: 7567 pFN21AE9674 7567 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3954 5377 278 Size: 53 83 186 936 2418 1423 2468 Fragments arranged by size: 2468 2418 1423 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4910 1937 Size: 2973 4594 Acc65I G'GTAC_C Cuts at: 4888 4888 Size: 7567 AclI AA'CG_TT Cuts at: 1563 5694 6067 1563 Size: 4131 373 3063 Fragments arranged by size: 4131 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2824 3303 3923 4143 4722 5630 7014 7282 Size: 1192 479 620 220 579 908 1384 268 Cuts at: 7282 1632 Size: 1917 Fragments arranged by size: 1917 1384 1192 908 620 579 479 268 220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7370 AflIII A'CryG_T Cuts at: 1203 6466 7443 1203 Size: 5263 977 1327 Fragments arranged by size: 5263 1327 977 AgeI A'CCGG_T Cuts at: 6398 6398 Size: 7567 AhdI GACnn_n'nnGTC Cuts at: 4803 6296 4803 Size: 1493 6074 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4107 1184 Size: 2923 4644 AlwNI CAG_nnn'CTG Cuts at: 2908 5310 6882 2908 Size: 2402 1572 3593 Fragments arranged by size: 3593 2402 1572 ApaLI G'TGCA_C Cuts at: 4457 5624 6780 4457 Size: 1167 1156 5244 Fragments arranged by size: 5244 1167 1156 ApoI r'AATT_y Cuts at: 1493 2170 4829 4876 5102 5254 7363 1493 Size: 677 2659 47 226 152 2109 1697 Fragments arranged by size: 2659 2109 1697 677 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 6121 160 Size: 872 5089 1606 Fragments arranged by size: 5089 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7567 AvaI C'yCGr_G Cuts at: 1870 1943 2116 4892 1870 Size: 73 173 2776 4545 Fragments arranged by size: 4545 2776 173 73 BamHI G'GATC_C Cuts at: 4897 5264 4897 Size: 367 7200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 3457 3936 4888 6343 Size: 325 217 627 333 1337 479 952 1455 Cuts at: 6343 618 Size: 1842 Fragments arranged by size: 1842 1455 1337 952 627 479 333 325 217 BanII G_rGCy'C Cuts at: 1391 3521 3774 4701 4886 1391 Size: 2130 253 927 185 4072 Fragments arranged by size: 4072 2130 927 253 185 BbeI G_GCGC'C Cuts at: 3940 3940 Size: 7567 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2671 3242 3401 928 Size: 971 772 571 159 5094 Fragments arranged by size: 5094 971 772 571 159 BbvCI CC'TCA_GC Cuts at: 4446 4446 Size: 7567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 5758 5792 1903 Size: 34 1580 34 2207 34 3678 Fragments arranged by size: 3678 2207 1580 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5459 6669 5459 Size: 1210 6357 BclI T'GATC_A Cuts at: 1548 3573 1548 Size: 2025 5542 BfrBI ATG'CAT Cuts at: 3009 3009 Size: 7567 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6178 136 Size: 107 122 71 631 605 4506 1525 Fragments arranged by size: 4506 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4647 7562 4647 Size: 2915 4652 BlpI GC'TnA_GC Cuts at: 4290 4410 4984 4290 Size: 120 574 6873 Fragments arranged by size: 6873 574 120 Bme1580I G_kGCm'C Cuts at: 1790 2123 2863 3746 4461 4514 5628 6784 1790 Size: 333 740 883 715 53 1114 1156 2573 Fragments arranged by size: 2573 1156 1114 883 740 715 333 53 BmgBI CAC'GTC Cuts at: 1318 2832 1318 Size: 1514 6053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 3808 4852 Size: 416 53 83 399 24 2138 253 1044 Cuts at: 4852 5298 6256 442 Size: 446 958 1753 Fragments arranged by size: 2138 1753 1044 958 446 416 399 253 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2956 2956 Size: 7567 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 2654 3004 3351 3935 6227 1239 Size: 1140 275 350 347 584 2292 2579 Fragments arranged by size: 2579 2292 1140 584 350 347 275 Bpu10I CC'TnA_GC Cuts at: 2448 4446 2448 Size: 1998 5569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2440 3215 3926 4259 5692 6557 6855 2440 Size: 775 711 333 1433 865 298 3152 Fragments arranged by size: 3152 1433 865 775 711 333 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4577 6230 882 Size: 617 3078 1653 2219 Fragments arranged by size: 3078 2219 1653 617 BsaAI yAC'GTr Cuts at: 493 1204 2345 4252 5358 493 Size: 711 1141 1907 1106 2702 Fragments arranged by size: 2702 1907 1141 1106 711 BsaBI GATnn'nnATC Cuts at: 7561 7561 Size: 7567 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 3951 5374 5756 Size: 53 83 186 936 2404 14 1423 382 Cuts at: 5756 6391 275 Size: 635 1451 Fragments arranged by size: 2404 1451 1423 936 635 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 3429 6000 6398 6672 6819 7230 3429 Size: 2571 398 274 147 411 3766 Fragments arranged by size: 3766 2571 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4289 4319 1178 Size: 30 165 30 2886 30 4426 Fragments arranged by size: 4426 2886 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2364 4375 1186 Size: 853 325 2011 4378 Fragments arranged by size: 4378 2011 853 325 BseYI C'CCAG_C Cuts at: 2801 3960 6770 7266 2801 Size: 1159 2810 496 3102 Fragments arranged by size: 3102 2810 1159 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4274 1163 Size: 573 2538 4456 Fragments arranged by size: 4456 2538 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 5017 5778 6806 1540 Size: 275 186 742 2274 761 1028 2301 Fragments arranged by size: 2301 2274 1028 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 3141 3774 4461 4701 5628 5713 6784 3141 Size: 633 687 240 927 85 1071 3924 Fragments arranged by size: 3924 1071 927 687 633 240 85 BsmI GAATG_Cn' Cuts at: 4488 5078 5171 4488 Size: 590 93 6884 Fragments arranged by size: 6884 590 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4863 4863 Size: 7567 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2863 3141 3521 3746 3774 4461 Size: 399 333 740 278 380 225 28 687 Cuts at: 4461 4514 4701 4886 5628 5713 6784 1391 Size: 53 187 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 740 687 399 380 333 278 225 187 185 85 53 28 BspEI T'CCGG_A Cuts at: 7230 7230 Size: 7567 BspHI T'CATG_A Cuts at: 2263 5456 7186 2263 Size: 3193 1730 2644 Fragments arranged by size: 3193 2644 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2907 4922 844 Size: 2063 2015 3489 Fragments arranged by size: 3489 2063 2015 BsrBI CCG'CTC Cuts at: 1394 1823 3445 4294 5454 1394 Size: 429 1622 849 1160 3507 Fragments arranged by size: 3507 1622 1160 849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2778 6062 6236 65 Size: 1183 879 651 3284 174 1396 Fragments arranged by size: 3284 1396 1183 879 651 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2963 3703 6211 6398 1700 Size: 110 1153 740 2508 187 2869 Fragments arranged by size: 2869 2508 1153 740 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7567 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7567 BssSI C'ACGA_G Cuts at: 3299 4499 5627 6639 3299 Size: 1200 1128 1012 4227 Fragments arranged by size: 4227 1200 1128 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7567 BstBI TT'CG_AA Cuts at: 6371 6371 Size: 7567 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7567 BstXI CCAn_nnnn'nTGG Cuts at: 4483 4483 Size: 7567 BstYI r'GATC_y Cuts at: 1972 4647 4897 5264 5649 5666 7118 7204 7216 Size: 2675 250 367 385 17 1452 86 12 Cuts at: 7216 7562 1972 Size: 346 1977 Fragments arranged by size: 2675 1977 1452 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2203 2889 4482 513 Size: 552 1138 686 1593 3598 Fragments arranged by size: 3598 1593 1138 686 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3019 3400 3772 487 Size: 683 56 1793 381 372 4282 Fragments arranged by size: 4282 1793 683 381 372 56 BtsI GCAGTG_nn' Cuts at: 2790 2956 5083 5877 5897 2790 Size: 166 2127 794 20 4460 Fragments arranged by size: 4460 2127 794 166 20 ClaI AT'CG_AT Cuts at: 1554 4269 1554 Size: 2715 4852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7532 DraI TTT'AAA Cuts at: 3076 3634 4871 3076 Size: 558 1237 5772 Fragments arranged by size: 5772 1237 558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6574 809 Size: 507 5258 1802 Fragments arranged by size: 5258 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3440 4341 Size: 54 1425 50 275 18 675 935 901 Cuts at: 4341 5014 5903 7532 8 Size: 673 889 1629 43 Fragments arranged by size: 1629 1425 935 901 889 675 673 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5014 1537 Size: 275 3202 4090 Fragments arranged by size: 4090 3202 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 4109 4171 5497 1353 Size: 687 2069 62 1326 3423 Fragments arranged by size: 3423 2069 1326 687 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6154 6528 6674 387 Size: 1064 138 4565 374 146 1280 Fragments arranged by size: 4565 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3772 4699 3772 Size: 927 6640 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2654 2824 3004 3303 3351 3923 Size: 393 747 275 170 180 299 48 572 Cuts at: 3923 3935 4143 4722 5630 6227 7014 7282 1239 Size: 12 208 579 908 597 787 268 1524 Fragments arranged by size: 1524 908 787 747 597 579 572 393 299 275 268 208 180 170 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 3914 4625 3914 Size: 711 6856 EcoO109I rG'GnC_Cy Cuts at: 2401 2685 3024 3058 4300 4630 2401 Size: 284 339 34 1242 330 5338 Fragments arranged by size: 5338 1242 339 330 284 34 EcoRI G'AATT_C Cuts at: 4876 5254 4876 Size: 378 7189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2202 3139 4592 2202 Size: 937 1453 5177 Fragments arranged by size: 5177 1453 937 FseI GG_CCGG'CC Cuts at: 2967 2967 Size: 7567 FspI TGC'GCA Cuts at: 6073 6073 Size: 7567 HaeII r_GCGC'y Cuts at: 1678 3940 6714 1678 Size: 2262 2774 2531 Fragments arranged by size: 2774 2531 2262 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 2997 3029 3087 Size: 32 135 32 949 32 444 32 58 Cuts at: 3087 3119 3318 3350 4775 4789 4807 4821 6208 Size: 32 199 32 1425 14 18 14 1387 Cuts at: 6208 6240 6282 6314 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 1387 949 444 199 135 58 42 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2896 4911 5157 669 Size: 988 281 958 2015 246 3079 Fragments arranged by size: 3079 2015 988 958 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7567 HpaI GTT'AAC Cuts at: 5157 5157 Size: 7567 KasI G'GCGC_C Cuts at: 3936 3936 Size: 7567 KpnI G_GTAC'C Cuts at: 4892 4892 Size: 7567 MfeI C'AATT_G Cuts at: 5166 5166 Size: 7567 MluI A'CGCG_T Cuts at: 7443 7443 Size: 7567 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3588 3912 3972 4305 4342 6681 6865 1305 Size: 2283 324 60 333 37 2339 184 2007 Fragments arranged by size: 2339 2283 2007 333 324 184 60 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 2507 10 Size: 54 1425 343 675 5070 Fragments arranged by size: 5070 1425 675 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 5525 5884 6043 518 Size: 648 1617 2742 359 159 2042 Fragments arranged by size: 2742 2042 1617 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3184 3566 3687 3964 4121 4289 4361 4517 Size: 1578 382 121 277 157 168 72 156 Cuts at: 4517 4983 5660 6808 7053 1606 Size: 466 677 1148 245 2120 Fragments arranged by size: 2120 1578 1148 677 466 382 277 245 168 157 156 121 72 NaeI GCC'GGC Cuts at: 2965 2965 Size: 7567 NarI GG'CG_CC Cuts at: 3937 3937 Size: 7567 NcoI C'CATG_G Cuts at: 513 1065 2203 513 Size: 552 1138 5877 Fragments arranged by size: 5877 1138 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7567 NgoMIV G'CCGG_C Cuts at: 2963 2963 Size: 7567 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7567 NotI GC'GGCC_GC Cuts at: 5014 5014 Size: 7567 NsiI A_TGCA'T Cuts at: 3011 3011 Size: 7567 NspI r_CATG'y Cuts at: 1132 1229 3013 4337 4925 6470 1132 Size: 97 1784 1324 588 1545 2229 Fragments arranged by size: 2229 1784 1545 1324 588 97 PasI CC'CwG_GG Cuts at: 1348 2559 4427 1348 Size: 1211 1868 4488 Fragments arranged by size: 4488 1868 1211 PciI A'CATG_T Cuts at: 6466 6466 Size: 7567 PflMI CCAn_nnn'nTGG Cuts at: 1108 2832 3117 4210 4768 1108 Size: 1724 285 1093 558 3907 Fragments arranged by size: 3907 1724 1093 558 285 PfoI T'CCnGG_A Cuts at: 4215 4701 4215 Size: 486 7081 PmeI GTTT'AAAC Cuts at: 4871 4871 Size: 7567 PmlI CAC'GTG Cuts at: 4252 4252 Size: 7567 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2453 2485 5636 5668 7149 7181 2453 Size: 32 3151 32 1481 32 2839 Fragments arranged by size: 3151 2839 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 2685 3058 4300 4630 2401 Size: 284 373 1242 330 5338 Fragments arranged by size: 5338 1242 373 330 284 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7567 PsiI TTA'TAA Cuts at: 5137 5137 Size: 7567 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2603 2635 2603 Size: 32 7535 PstI C_TGCA'G Cuts at: 830 2904 4045 4919 830 Size: 2074 1141 874 3478 Fragments arranged by size: 3478 2074 1141 874 PvuI CG_AT'CG Cuts at: 2001 2743 2001 Size: 742 6825 PvuII CAG'CTG Cuts at: 3184 3566 3964 4121 4289 4517 3184 Size: 382 398 157 168 228 6234 Fragments arranged by size: 6234 398 382 228 168 157 SacI G_AGCT'C Cuts at: 3774 4701 3774 Size: 927 6640 SalI G'TCGA_C Cuts at: 1936 4909 1936 Size: 2973 4594 SbfI CC_TGCA'GG Cuts at: 4919 4919 Size: 7567 ScaI AGT'ACT Cuts at: 5815 7527 5815 Size: 1712 5855 SexAI A'CCwGG_T Cuts at: 4395 4395 Size: 7567 SfcI C'TryA_G Cuts at: 826 1045 2854 2900 4041 4915 6050 6731 6922 Size: 219 1809 46 1141 874 1135 681 191 Cuts at: 6922 826 Size: 1471 Fragments arranged by size: 1809 1471 1141 1135 874 681 219 191 46 SfoI GGC'GCC Cuts at: 3938 3938 Size: 7567 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7567 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7567 SmaI CCC'GGG Cuts at: 1872 4894 1872 Size: 3022 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 3230 3941 4238 5671 6572 Size: 197 926 476 811 711 297 1433 901 Cuts at: 6572 6834 820 Size: 262 1553 Fragments arranged by size: 1553 1433 926 901 811 711 476 297 262 197 SnaBI TAC'GTA Cuts at: 493 5358 493 Size: 4865 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7567 SphI G_CATG'C Cuts at: 1132 3013 4337 4925 1132 Size: 1881 1324 588 3774 Fragments arranged by size: 3774 1881 1324 588 SspI AAT'ATT Cuts at: 5 52 5491 5 Size: 47 5439 2081 Fragments arranged by size: 5439 2081 47 StuI AGG'CCT Cuts at: 1517 3491 1517 Size: 1974 5593 StyI C'CwwG_G Cuts at: 513 1065 2177 2203 2379 3860 4047 4062 5006 Size: 552 1112 26 176 1481 187 15 944 Cuts at: 5006 513 Size: 3074 Fragments arranged by size: 3074 1481 1112 944 552 187 176 26 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5594 5611 5764 5949 6394 325 Size: 1255 4014 17 153 185 445 1498 Fragments arranged by size: 4014 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5813 7525 96 Size: 274 80 33 51 1443 3836 1712 138 Fragments arranged by size: 3836 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4289 4321 7527 7559 4289 Size: 32 3206 32 4297 Fragments arranged by size: 4297 3206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7567 XbaI T'CTAG_A Cuts at: 4903 4903 Size: 7567 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2756 4030 2756 Size: 1274 6293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7567 XmaI C'CCGG_G Cuts at: 1870 4892 1870 Size: 3022 4545 XmnI GAAnn'nnTTC Cuts at: 4735 4756 5696 4735 Size: 21 940 6606 Fragments arranged by size: 6606 940 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3952 5375 276 Size: 53 83 186 936 2418 1423 2468 Fragments arranged by size: 2468 2418 1423 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI NruI PacI PspOMI PspXI RsrII SacII SanDI SapI SfiI SrfI SwaI