(Linear) (Six-base) MAPSORT of: KIEE9674.seq Check: 9639 from: 1 to: 2862 KIEE9674.orf With 182 enzymes: SgfI * December 27, 2011 16:43 .. AatII G_ACGT'C Cuts at: 0 1949 2862 Size: 1949 913 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 819 1298 1918 2138 2717 2862 Size: 819 479 620 220 579 145 Fragments arranged by size: 819 620 579 479 220 145 AhdI GACnn_n'nnGTC Cuts at: 0 2798 2862 Size: 2798 64 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2102 2862 Size: 2102 760 AlwNI CAG_nnn'CTG Cuts at: 0 903 2862 Size: 903 1959 ApaLI G'TGCA_C Cuts at: 0 2452 2862 Size: 2452 410 ApoI r'AATT_y Cuts at: 0 165 2824 2862 Size: 165 2659 38 Fragments arranged by size: 2659 165 38 AvaI C'yCGr_G Cuts at: 0 111 2862 Size: 111 2751 BanI G'GyrC_C Cuts at: 0 115 1452 1931 2862 Size: 115 1337 479 931 Fragments arranged by size: 1337 931 479 115 BanII G_rGCy'C Cuts at: 0 1516 1769 2696 2862 Size: 1516 253 927 166 Fragments arranged by size: 1516 927 253 166 BbeI G_GCGC'C Cuts at: 0 1935 2862 Size: 1935 927 BbsI GAAGACnn'nnnn_ Cuts at: 0 666 1237 1396 2862 Size: 666 571 159 1466 Fragments arranged by size: 1466 666 571 159 BbvCI CC'TCA_GC Cuts at: 0 2441 2862 Size: 2441 421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1512 1546 2862 Size: 1512 34 1316 Fragments arranged by size: 1512 1316 34 BclI T'GATC_A Cuts at: 0 1568 2862 Size: 1568 1294 BfrBI ATG'CAT Cuts at: 0 1004 2862 Size: 1004 1858 BglII A'GATC_T Cuts at: 0 2642 2862 Size: 2642 220 BlpI GC'TnA_GC Cuts at: 0 2285 2405 2862 Size: 2285 120 457 Fragments arranged by size: 2285 457 120 Bme1580I G_kGCm'C Cuts at: 0 118 858 1741 2456 2509 2862 Size: 118 740 883 715 53 353 Fragments arranged by size: 883 740 715 353 118 53 BmgBI CAC'GTC Cuts at: 0 827 2862 Size: 827 2035 BmrI ACTGGGnnnn_n' Cuts at: 0 1550 1803 2847 2862 Size: 1550 253 1044 15 Fragments arranged by size: 1550 1044 253 15 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 951 2862 Size: 951 1911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 649 999 1346 1930 2862 Size: 374 275 350 347 584 932 Fragments arranged by size: 932 584 374 350 347 275 Bpu10I CC'TnA_GC Cuts at: 0 443 2441 2862 Size: 443 1998 421 Fragments arranged by size: 1998 443 421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 435 1210 1921 2254 2862 Size: 435 775 711 333 608 Fragments arranged by size: 775 711 608 435 333 BsaI GGTCTCn'nnnn_ Cuts at: 0 2572 2862 Size: 2572 290 BsaAI yAC'GTr Cuts at: 0 340 2247 2862 Size: 340 1907 615 Fragments arranged by size: 1907 615 340 BsaHI Gr'CG_yC Cuts at: 0 1932 1946 2862 Size: 1932 14 916 Fragments arranged by size: 1932 916 14 BsaWI w'CCGG_w Cuts at: 0 1424 2862 Size: 1424 1438 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2284 2314 2862 Size: 2284 30 548 Fragments arranged by size: 2284 548 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 359 2370 2862 Size: 34 325 2011 492 Fragments arranged by size: 2011 492 325 34 BseYI C'CCAG_C Cuts at: 0 796 1955 2862 Size: 796 1159 907 Fragments arranged by size: 1159 907 796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2269 2862 Size: 2269 593 BsiEI CG_ry'CG Cuts at: 0 738 2862 Size: 738 2124 BsiHKAI G_wGCw'C Cuts at: 0 1136 1769 2456 2696 2862 Size: 1136 633 687 240 166 Fragments arranged by size: 1136 687 633 240 166 BsmI GAATG_Cn' Cuts at: 0 2483 2862 Size: 2483 379 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2858 2862 Size: 2858 4 Bsp1286I G_dGCh'C Cuts at: 0 118 858 1136 1516 1741 1769 2456 2509 Size: 118 740 278 380 225 28 687 53 Cuts at: 2509 2696 2862 Size: 187 166 Fragments arranged by size: 740 687 380 278 225 187 166 118 53 28 BspHI T'CATG_A Cuts at: 0 258 2862 Size: 258 2604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 902 2862 Size: 902 1960 BsrBI CCG'CTC Cuts at: 0 1440 2289 2862 Size: 1440 849 573 Fragments arranged by size: 1440 849 573 BsrDI GCAATG_nn' Cuts at: 0 122 773 2862 Size: 122 651 2089 Fragments arranged by size: 2089 651 122 BsrFI r'CCGG_y Cuts at: 0 958 1698 2862 Size: 958 740 1164 Fragments arranged by size: 1164 958 740 BssSI C'ACGA_G Cuts at: 0 1294 2494 2862 Size: 1294 1200 368 Fragments arranged by size: 1294 1200 368 BstXI CCAn_nnnn'nTGG Cuts at: 0 2478 2862 Size: 2478 384 BstYI r'GATC_y Cuts at: 0 2642 2862 Size: 2642 220 BtgI C'CryG_G Cuts at: 0 198 884 2477 2862 Size: 198 686 1593 385 Fragments arranged by size: 1593 686 385 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1014 1395 1767 2862 Size: 1014 381 372 1095 Fragments arranged by size: 1095 1014 381 372 BtsI GCAGTG_nn' Cuts at: 0 785 951 2862 Size: 785 166 1911 Fragments arranged by size: 1911 785 166 ClaI AT'CG_AT Cuts at: 0 2264 2862 Size: 2264 598 DraI TTT'AAA Cuts at: 0 1071 1629 2862 Size: 1071 558 1233 Fragments arranged by size: 1233 1071 558 EaeI y'GGCC_r Cuts at: 0 500 1435 2336 2862 Size: 500 935 901 526 Fragments arranged by size: 935 901 526 500 EarI CTCTTCn'nnn_ Cuts at: 0 35 2104 2166 2862 Size: 35 2069 62 696 Fragments arranged by size: 2069 696 62 35 EcoICRI GAG'CTC Cuts at: 0 1767 2694 2862 Size: 1767 927 168 Fragments arranged by size: 1767 927 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 649 819 999 1298 1346 1918 1930 Size: 374 275 170 180 299 48 572 12 Cuts at: 1930 2138 2717 2862 Size: 208 579 145 Fragments arranged by size: 579 572 374 299 275 208 180 170 145 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1909 2620 2862 Size: 1909 711 242 Fragments arranged by size: 1909 711 242 EcoO109I rG'GnC_Cy Cuts at: 0 396 680 1019 1053 2295 2625 2862 Size: 396 284 339 34 1242 330 237 Fragments arranged by size: 1242 396 339 330 284 237 34 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 197 1134 2587 2862 Size: 197 937 1453 275 Fragments arranged by size: 1453 937 275 197 FseI GG_CCGG'CC Cuts at: 0 962 2862 Size: 962 1900 HaeII r_GCGC'y Cuts at: 0 1935 2862 Size: 1935 927 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 516 548 992 1024 1082 1114 1313 1345 Size: 516 32 444 32 58 32 199 32 Cuts at: 1345 2770 2784 2802 2816 2862 Size: 1425 14 18 14 46 Fragments arranged by size: 1425 516 444 199 58 46 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 0 891 2862 Size: 891 1971 KasI G'GCGC_C Cuts at: 0 1931 2862 Size: 1931 931 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1583 1907 1967 2300 2337 2862 Size: 1583 324 60 333 37 525 Fragments arranged by size: 1583 525 333 324 60 37 MscI TGG'CCA Cuts at: 0 502 2862 Size: 502 2360 MslI CAynn'nnrTG Cuts at: 0 778 2862 Size: 778 2084 MspA1I CmG'CkG Cuts at: 0 1179 1561 1682 1959 2116 2284 2356 2512 Size: 1179 382 121 277 157 168 72 156 Cuts at: 2512 2862 Size: 350 Fragments arranged by size: 1179 382 350 277 168 157 156 121 72 NaeI GCC'GGC Cuts at: 0 960 2862 Size: 960 1902 NarI GG'CG_CC Cuts at: 0 1932 2862 Size: 1932 930 NcoI C'CATG_G Cuts at: 0 198 2862 Size: 198 2664 NgoMIV G'CCGG_C Cuts at: 0 958 2862 Size: 958 1904 NsiI A_TGCA'T Cuts at: 0 1006 2862 Size: 1006 1856 NspI r_CATG'y Cuts at: 0 1008 2332 2862 Size: 1008 1324 530 Fragments arranged by size: 1324 1008 530 PasI CC'CwG_GG Cuts at: 0 554 2422 2862 Size: 554 1868 440 Fragments arranged by size: 1868 554 440 PflMI CCAn_nnn'nTGG Cuts at: 0 827 1112 2205 2763 2862 Size: 827 285 1093 558 99 Fragments arranged by size: 1093 827 558 285 99 PfoI T'CCnGG_A Cuts at: 0 2210 2696 2862 Size: 2210 486 166 Fragments arranged by size: 2210 486 166 PmlI CAC'GTG Cuts at: 0 2247 2862 Size: 2247 615 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 2862 Size: 448 32 2382 Fragments arranged by size: 2382 448 32 PpuMI rG'GwC_Cy Cuts at: 0 396 680 1053 2295 2625 2862 Size: 396 284 373 1242 330 237 Fragments arranged by size: 1242 396 373 330 284 237 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 598 630 2862 Size: 598 32 2232 Fragments arranged by size: 2232 598 32 PstI C_TGCA'G Cuts at: 0 899 2040 2862 Size: 899 1141 822 Fragments arranged by size: 1141 899 822 PvuI CG_AT'CG Cuts at: 0 738 2862 Size: 738 2124 PvuII CAG'CTG Cuts at: 0 1179 1561 1959 2116 2284 2512 2862 Size: 1179 382 398 157 168 228 350 Fragments arranged by size: 1179 398 382 350 228 168 157 SacI G_AGCT'C Cuts at: 0 1769 2696 2862 Size: 1769 927 166 Fragments arranged by size: 1769 927 166 SexAI A'CCwGG_T Cuts at: 0 2390 2862 Size: 2390 472 SfcI C'TryA_G Cuts at: 0 849 895 2036 2862 Size: 849 46 1141 826 Fragments arranged by size: 1141 849 826 46 SfoI GGC'GCC Cuts at: 0 1933 2862 Size: 1933 929 SmlI C'TyrA_G Cuts at: 0 414 1225 1936 2233 2862 Size: 414 811 711 297 629 Fragments arranged by size: 811 711 629 414 297 SphI G_CATG'C Cuts at: 0 1008 2332 2862 Size: 1008 1324 530 Fragments arranged by size: 1324 1008 530 StuI AGG'CCT Cuts at: 0 1486 2862 Size: 1486 1376 StyI C'CwwG_G Cuts at: 0 172 198 374 1855 2042 2057 2862 Size: 172 26 176 1481 187 15 805 Fragments arranged by size: 1481 805 187 176 172 26 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2284 2316 2862 Size: 2284 32 546 Fragments arranged by size: 2284 546 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 751 2025 2862 Size: 751 1274 837 Fragments arranged by size: 1274 837 751 XmnI GAAnn'nnTTC Cuts at: 0 2730 2751 2862 Size: 2730 21 111 Fragments arranged by size: 2730 111 21 ZraI GAC'GTC Cuts at: 0 1947 2862 Size: 1947 915 Enzymes that do cut: AatII AcuI AhdI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PsrI PstI PvuI PvuII SacI SexAI SfcI SfoI SmlI SphI StuI StyI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BciVI BglI BmtI BsaBI BsiWI BspEI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraIII DrdI EagI EciI EcoRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI PacI PciI PmeI PshAI PsiI PspOMI PspXI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI