Restriction map for
pFN21AB5364
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BmyI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstEII
BstPI BstYI Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HphI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1105 GTATAC GT^[AC][GT]AC
AclI 674 AACGTT AA^CGTT
Asp700I 712 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 118 GGACC G^G[AT]CC
BanI 207 GGCACC G^G[CT][GA]CC
BcnI 4 CCGGG CC^[GC]GG
BglI 308 GCCTACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 925 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 925 GCTAAGC GC^T[ACGT]AGC
BsgI 134 GTGCAG GTGCAG(16/14)
BsmAI 887 GAGAC GTCTC(1/5)
BsmFI 1157 GGGAC GGGAC(10/14)
BsrI 858 CCAGT ACTGG(1/-1)
BssSI 884 CACGAG CACGAG(-5/-1)
Bst1107I 1105 GTATAC GTA^TAC
BstBI 1141 TTCGAA TT^CGAA
BstXI 818 CCAAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1105 GTATAC GTA^TAC
CelII 925 GCTAAGC GC^T[ACGT]AGC
DpnII 994 GATC ^GATC
DrdI 650 GACTGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 337 GAAGAG CTCTTC(1/4)
EcoRI 231 GAATTC G^AATTC
EcoT14I 142 CCATGG C^C[AT][AT]GG
FauI 1155 GCGGG CCCGC(4/6)
HgaI 17 GCGTC GACGC(5/10)
HindIII 606 AAGCTT A^AGCTT
HpaI 630 GTTAAC GTT^AAC
Ksp632I 337 GAAGAG CTCTTC(1/4)
MboI 994 GATC ^GATC
NciI 4 CCGGG CC^[GC]GG
NcoI 142 CCATGG C^CATGG
NdeII 994 GATC ^GATC
NspI 626 GCATGT [GA]CATG^[CT]
NspV 1141 TTCGAA TT^CGAA
Psp1406I 674 AACGTT AA^CGTT
PstI 90 CTGCAG CTGCA^G
PvuII 48 CAGCTG CAG^CTG
Sau3AI 994 GATC ^GATC
SfcI 90 CTGCAG C^T[GA][CT]AG
SfuI 1141 TTCGAA TT^CGAA
SspI 400 AATATT AAT^ATT
StyI 142 CCATGG C^C[AT][AT]GG
XbaI 708 TCTAGA T^CTAGA
XmnI 712 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 60 CGCG CG^CG
69 CGCG
BbsI 445 GAAGAC GAAGAC(2/6)
592 GTCTTC
BbvI 63 GCAGC GCAGC(8/12)
37 GCTGC
BfaI 709 CTAG C^TAG
758 CTAG
BpuAI 445 GAAGAC GAAGAC(2/6)
592 GTCTTC
BspMI 1151 ACCTGC ACCTGC(4/8)
282 GCAGGT
BstUI 60 CGCG CG^CG
69 CGCG
CfoI 61 GCGC GCG^C
70 GCGC
DsaI 76 CCGTGG C^C[GA][CT]GG
142 CCATGG
EcoNI 109 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1171 CCTTGTATAGG
HapII 4 CCGG C^CGG
9 CCGG
HhaI 61 GCGC GCG^C
70 GCGC
HinP1I 61 GCGC G^CGC
70 GCGC
HincII 630 GTTAAC GT[CT]^[GA]AC
1074 GTCAAC
HindII 630 GTTAAC GT[CT]^[GA]AC
1074 GTCAAC
HpaII 4 CCGG C^CGG
9 CCGG
MaeI 709 CTAG C^TAG
758 CTAG
MaeII 124 ACGT A^CGT
675 ACGT
MaeIII 364 GTTAC ^GT[ACGT]AC
1126 GTTAC
MseI 553 TTAA T^TAA
631 TTAA
MspA1I 48 CAGCTG C[AC]G^C[GT]G
359 CCGCTG
MspI 4 CCGG C^CGG
9 CCGG
MvnI 60 CGCG CG^CG
69 CGCG
TaqI 920 TCGA T^CGA
1142 TCGA
Tru9I 553 TTAA T^TAA
631 TTAA
TseI 37 GCTGC G^C[AT]GC
63 GCAGC
TspRI 557 CAGTG CA[GC]TG[ACGT][ACGT]^
665 CACTG
TthHB8I 920 TCGA T^CGA
1142 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MboII MnlI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TfiI Tsp509I