(Circular) (Six-base) MAPSORT of: pFN21AB5364.seq Check: 6692 from: 1 to: 5890 pFN21AB5364 5890 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2278 2278 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3111 3233 1937 Size: 1174 122 4594 Fragments arranged by size: 4594 1174 122 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 2680 4017 4390 1563 Size: 1117 1337 373 3063 Fragments arranged by size: 3063 1337 1117 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 2264 3953 5337 5605 1632 Size: 471 161 1689 1384 268 1917 Fragments arranged by size: 1917 1689 1384 471 268 161 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 3633 5205 3633 Size: 1572 4318 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2236 2566 2806 3199 3425 3577 5686 1493 Size: 743 330 240 393 226 152 2109 1697 Fragments arranged by size: 2109 1697 743 393 330 240 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2517 2550 2517 Size: 33 5857 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 3211 4666 618 Size: 325 217 627 425 999 1455 1842 Fragments arranged by size: 1842 1455 999 627 425 325 217 BanII G_rGCy'C Cuts at: 1391 3209 1391 Size: 1818 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 2590 928 Size: 971 558 133 4228 Fragments arranged by size: 4228 971 558 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4081 4115 1903 Size: 34 50 34 2060 34 3678 Fragments arranged by size: 3678 2060 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 4501 136 Size: 107 122 71 631 605 647 2182 1525 Fragments arranged by size: 2182 1525 647 631 605 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 2931 3307 2931 Size: 376 5514 Bme1580I G_kGCm'C Cuts at: 1790 3951 5107 1790 Size: 2161 1156 2573 Fragments arranged by size: 2573 2161 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4550 1239 Size: 3311 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4015 4880 5178 4015 Size: 865 298 4727 Fragments arranged by size: 4727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4553 882 Size: 617 3054 2219 Fragments arranged by size: 3054 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2098 2128 1178 Size: 30 165 30 695 30 4940 Fragments arranged by size: 4940 695 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5890 BseYI C'CCAG_C Cuts at: 2836 5093 5589 2836 Size: 2257 496 3137 Fragments arranged by size: 3137 2257 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 1163 Size: 573 424 4893 Fragments arranged by size: 4893 573 424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3340 4101 5129 1540 Size: 275 186 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3951 4036 5107 3951 Size: 85 1071 4734 Fragments arranged by size: 4734 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3209 3951 4036 5107 1391 Size: 399 1419 742 85 1071 2174 Fragments arranged by size: 2174 1419 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2278 3165 3245 844 Size: 1434 887 80 3489 Fragments arranged by size: 3489 1434 887 80 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4534 4721 1700 Size: 110 2724 187 2869 Fragments arranged by size: 2869 2724 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 2889 3950 4962 2889 Size: 1061 1012 3817 Fragments arranged by size: 3817 1061 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 3147 4694 3147 Size: 1547 4343 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2830 2830 Size: 5890 BstYI r'GATC_y Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885 Size: 1248 367 385 17 1452 86 12 346 Cuts at: 5885 1972 Size: 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3112 3112 Size: 5890 BtgI C'CryG_G Cuts at: 513 1065 2081 2147 513 Size: 552 1016 66 4256 Fragments arranged by size: 4256 1016 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2661 4897 809 Size: 507 1345 2236 1802 Fragments arranged by size: 2236 1802 1345 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855 Size: 54 1425 50 275 18 1507 889 1629 Cuts at: 5855 8 Size: 43 Fragments arranged by size: 1629 1507 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 3820 1353 Size: 984 1483 3423 Fragments arranged by size: 3423 1483 984 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2264 3953 4550 5337 5605 1239 Size: 393 471 161 1689 597 787 268 1524 Fragments arranged by size: 1689 1524 787 597 471 393 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2118 3180 2118 Size: 1062 4828 EcoRI G'AATT_C Cuts at: 2236 3199 3577 2236 Size: 963 378 4549 Fragments arranged by size: 4549 963 378 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3062 3094 4531 4563 4605 Size: 32 135 32 1490 32 1437 32 42 Cuts at: 4605 4637 1373 Size: 32 2626 Fragments arranged by size: 2626 1490 1437 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 3081 3234 3480 669 Size: 988 281 699 444 153 246 3079 Fragments arranged by size: 3079 988 699 444 281 246 153 HindIII A'AGCT_T Cuts at: 748 2611 748 Size: 1863 4027 HpaI GTT'AAC Cuts at: 2637 3480 2637 Size: 843 5047 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2954 5004 5188 1305 Size: 1649 2050 184 2007 Fragments arranged by size: 2050 2007 1649 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4068 Fragments arranged by size: 4068 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3848 4207 4366 518 Size: 648 2682 359 159 2042 Fragments arranged by size: 2682 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2366 3306 3983 5131 5376 1606 Size: 449 311 940 677 1148 245 2120 Fragments arranged by size: 2120 1148 940 677 449 311 245 NcoI C'CATG_G Cuts at: 513 1065 2147 513 Size: 552 1082 4256 Fragments arranged by size: 4256 1082 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2635 3248 4793 1132 Size: 97 1406 613 1545 2229 Fragments arranged by size: 2229 1545 1406 613 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5890 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2127 2159 3959 3991 5472 5504 2127 Size: 32 1800 32 1481 32 2513 Fragments arranged by size: 2513 1800 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PstI C_TGCA'G Cuts at: 830 2099 3242 830 Size: 1269 1143 3478 Fragments arranged by size: 3478 1269 1143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2055 2055 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2095 3238 4373 5054 5245 826 Size: 219 1050 1143 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 1050 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3994 4895 5157 820 Size: 197 926 2051 901 262 1553 Fragments arranged by size: 2051 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 2407 3814 5 Size: 47 2355 1407 2081 Fragments arranged by size: 2355 2081 1407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2147 3329 513 Size: 552 1082 1182 3074 Fragments arranged by size: 3074 1182 1082 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2496 3917 3934 4087 4272 4717 325 Size: 1255 916 1421 17 153 185 445 1498 Fragments arranged by size: 1498 1421 1255 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2432 4136 5848 Size: 274 80 33 51 1443 455 1704 1712 Cuts at: 5848 96 Size: 138 Fragments arranged by size: 1712 1704 1443 455 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 2713 3226 2713 Size: 513 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 2721 4019 2721 Size: 1298 4592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI