################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI MamI MluI MroI NarI NdeI NheI NotI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 241 GACGCC G[GA]^CG[CT]C AflII 1662 CTTAAG C^TTAAG Asp700I 381 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1272 GGATCC G^GATCC BanI 258 GGCACC G^G[CT][GA]CC BanII 88 GAGCCC G[GA]GC[CT]^C BciVI 1454 GGATAC GTATCC(6/5) BfrI 1662 CTTAAG C^TTAAG BlpI 1681 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1681 GCTCAGC GC^T[ACGT]AGC BsaAI 565 CACGTA [CT]AC^GT[GA] BsaHI 241 GACGCC G[GA]^CG[CT]C BsmFI 514 GGGAC GGGAC(10/14) BspMI 1308 ACCTGC ACCTGC(4/8) BsrDI 1325 GCAATG GCAATG(2/0) CelII 1681 GCTCAGC GC^T[ACGT]AGC DraI 1600 TTTAAA TTT^AAA DraII 247 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 821 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 883 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1615 GAAGAG CTCTTC(1/4) EcoO109I 247 GGGGCCT [GA]G^G[ACGT]CC[CT] Hin1I 241 GACGCC G[GA]^CG[CT]C HincII 1545 GTCAAC GT[CT]^[GA]AC HindII 1545 GTCAAC GT[CT]^[GA]AC Ksp632I 1615 GAAGAG CTCTTC(1/4) MfeI 1484 CAATTG C^AATTG MunI 1484 CAATTG C^AATTG NcoI 1276 CCATGG C^CATGG NruI 225 TCGCGA TCG^CGA PaeR7I 1370 CTCGAG C^TCGAG PflFI 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 1358 CAGCTG CAG^CTG SmaI 347 CCCGGG CCC^GGG SpeI 1102 ACTAGT A^CTAGT Tth111I 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1758 CCAGAAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1370 CTCGAG C^TCGAG XmaCI 347 CCCGGG C^CCGGG XmaI 347 CCCGGG C^CCGGG XmnI 381 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 12 GTAC GT^AC 57 GTAC BbsI 31 GTCTTC GAAGAC(2/6) 497 GTCTTC BpuAI 31 GTCTTC GAAGAC(2/6) 497 GTCTTC BseRI 997 GAGGAG GAGGAG(10/8) 1267 GAGGAG BstAPI 337 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1077 GCAGCGTATGC BstYI 1272 GGATCC [GA]^GATC[CT] 1407 AGATCC CfoI 416 GCGC GCG^C 1433 GCGC DpnII 1273 GATC ^GATC 1408 GATC EagI 154 CGGCCG C^GGCCG 164 CGGCCG EclXI 154 CGGCCG C^GGCCG 164 CGGCCG Eco52I 154 CGGCCG C^GGCCG 164 CGGCCG EcoRI 593 GAATTC G^AATTC 1290 GAATTC HhaI 416 GCGC GCG^C 1433 GCGC HinP1I 416 GCGC G^CGC 1433 GCGC KspI 121 CCGCGG CCGC^GG 151 CCGCGG MaeII 68 ACGT A^CGT 566 ACGT MboI 1273 GATC ^GATC 1408 GATC MflI 1272 GGATCC [GA]^GATC[CT] 1407 AGATCC NdeII 1273 GATC ^GATC 1408 GATC RsaI 12 GTAC GT^AC 57 GTAC SacII 121 CCGCGG CCGC^GG 151 CCGCGG Sau3AI 1273 GATC ^GATC 1408 GATC SfaNI 970 GATGC GCATC(5/9) 1121 GCATC SmlI 1370 CTCGAG C^T[CT][GA]AG 1662 CTTAAG XhoII 1272 GGATCC [GA]^GATC[CT] 1407 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AspHI AvaI AvaII BalI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr10I Cfr13I DdeI DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI Sau96I ScrFI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I