(Circular) (Six-base) MAPSORT of: pFN21AE6312.seq Check: 3463 from: 1 to: 5647 pFN21AE6312 5647 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2381 2381 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2387 2815 3710 5094 5362 1632 Size: 608 147 428 895 1384 268 1917 Fragments arranged by size: 1917 1384 895 608 428 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2673 1166 Size: 1507 4140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2786 2818 2786 Size: 32 5615 AlwNI CAG_nnn'CTG Cuts at: 2128 3390 4962 2128 Size: 1262 1572 2813 Fragments arranged by size: 2813 1572 1262 ApaI G_GGCC'C Cuts at: 2876 2876 Size: 5647 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2520 2571 2972 1870 Size: 73 235 342 51 401 4545 Fragments arranged by size: 4545 401 342 235 73 51 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2393 2402 2968 4423 618 Size: 325 217 627 606 9 566 1455 1842 Fragments arranged by size: 1842 1455 627 606 566 325 217 9 BanII G_rGCy'C Cuts at: 1391 2876 2966 1391 Size: 1485 90 4072 Fragments arranged by size: 4072 1485 90 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2225 3539 4749 2225 Size: 1314 1210 3123 Fragments arranged by size: 3123 1314 1210 BclI T'GATC_A Cuts at: 1548 2685 1548 Size: 1137 4510 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2238 2429 Size: 107 122 71 631 605 337 229 191 Cuts at: 2429 4258 136 Size: 1829 1525 Fragments arranged by size: 1829 1525 631 605 337 229 191 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2876 3708 4864 1790 Size: 1086 832 1156 2573 Fragments arranged by size: 2573 1156 1086 832 BmgBI CAC'GTC Cuts at: 1318 2797 1318 Size: 1479 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2259 2343 3378 Size: 416 53 83 399 24 842 84 1035 Cuts at: 3378 4336 442 Size: 958 1753 Fragments arranged by size: 1753 1035 958 842 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4307 1239 Size: 3068 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 2513 5641 2513 Size: 3128 2519 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2145 4080 4478 4752 4899 5310 2145 Size: 1935 398 274 147 411 2482 Fragments arranged by size: 2482 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 1178 Size: 30 165 30 694 30 4698 Fragments arranged by size: 4698 694 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2595 4850 5346 2595 Size: 2255 496 2896 Fragments arranged by size: 2896 2255 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5074 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3708 3793 4864 3708 Size: 85 1071 4491 Fragments arranged by size: 4491 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 BsmBI CGTCTCn'nnnn_ Cuts at: 2936 2936 Size: 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2876 2966 3708 3793 4864 1391 Size: 399 1086 90 742 85 1071 2174 Fragments arranged by size: 2174 1086 1071 742 399 90 85 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 3002 844 Size: 1537 621 3489 Fragments arranged by size: 3489 1537 621 BsrBI CCG'CTC Cuts at: 1394 1823 2160 3534 1394 Size: 429 337 1374 3507 Fragments arranged by size: 3507 1374 429 337 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 4142 4316 65 Size: 1183 1479 1415 174 1396 Fragments arranged by size: 1479 1415 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2457 4291 4478 1700 Size: 110 220 427 1834 187 2869 Fragments arranged by size: 2869 1834 427 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 2512 4451 2512 Size: 1939 3708 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2722 2480 Size: 242 5405 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2234 2234 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 2004 2479 2565 513 Size: 552 939 475 86 3595 Fragments arranged by size: 3595 939 552 475 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2703 3163 3957 3977 2703 Size: 460 794 20 4373 Fragments arranged by size: 4373 794 460 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DraIII CAC_nnn'GTG Cuts at: 2544 2544 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2455 3094 Size: 54 1425 50 275 18 264 361 639 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 639 361 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 2939 3577 1353 Size: 1167 419 638 3423 Fragments arranged by size: 3423 1167 638 419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2387 2815 3710 4307 5094 5362 Size: 393 608 147 428 895 597 787 268 Cuts at: 5362 1239 Size: 1524 Fragments arranged by size: 1524 895 787 608 597 428 393 268 147 EcoNI CCTnn'n_nnAGG Cuts at: 2840 2840 Size: 5647 EcoO109I rG'GnC_Cy Cuts at: 2089 2873 2089 Size: 784 4863 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2450 2644 2950 2450 Size: 194 306 5147 Fragments arranged by size: 5147 306 194 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2329 2479 4794 1678 Size: 651 150 2315 2531 Fragments arranged by size: 2531 2315 651 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2677 2709 4288 4320 4362 Size: 32 135 32 1105 32 1579 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 1579 1105 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2673 2720 2756 3605 3964 4123 518 Size: 648 1507 47 36 849 359 159 2042 Fragments arranged by size: 2042 1507 849 648 359 159 47 36 MspA1I CmG'CkG Cuts at: 1606 2468 2691 3063 3740 4888 5133 1606 Size: 862 223 372 677 1148 245 2120 Fragments arranged by size: 2120 1148 862 677 372 245 223 NaeI GCC'GGC Cuts at: 2032 2459 2032 Size: 427 5220 NcoI C'CATG_G Cuts at: 513 1065 2004 2565 513 Size: 552 939 561 3595 Fragments arranged by size: 3595 939 561 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NgoMIV G'CCGG_C Cuts at: 2030 2457 2030 Size: 427 5220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2755 3005 4550 1132 Size: 97 1526 250 1545 2229 Fragments arranged by size: 2229 1545 1526 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 1108 Size: 1020 4627 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2872 2872 Size: 5647 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2906 2938 2906 Size: 32 5615 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2468 2468 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SexAI A'CCwGG_T Cuts at: 2133 2133 Size: 5647 SfcI C'TryA_G Cuts at: 826 1045 2109 2995 4130 4811 5002 826 Size: 219 1064 886 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 886 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2522 2974 1872 Size: 650 452 4545 Fragments arranged by size: 4545 650 452 SmlI C'TyrA_G Cuts at: 820 1017 1943 2178 3751 4652 4914 820 Size: 197 926 235 1573 901 262 1553 Fragments arranged by size: 1573 1553 926 901 262 235 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2232 2921 1517 Size: 715 689 4243 Fragments arranged by size: 4243 715 689 StyI C'CwwG_G Cuts at: 513 1065 2004 2299 2565 3086 513 Size: 552 939 295 266 521 3074 Fragments arranged by size: 3074 939 552 521 295 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3674 3691 3844 4029 4474 325 Size: 1255 2094 17 153 185 445 1498 Fragments arranged by size: 2094 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3893 5605 Size: 274 80 33 51 1443 207 1709 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5607 5639 5607 Size: 32 5615 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 2178 1943 Size: 235 5412 XmaI C'CCGG_G Cuts at: 1870 2520 2972 1870 Size: 650 452 4545 Fragments arranged by size: 4545 650 452 XmnI GAAnn'nnTTC Cuts at: 2846 3776 2846 Size: 930 4717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI